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Yorodumi- PDB-1dvp: CRYSTAL STRUCTURE OF THE VHS AND FYVE TANDEM DOMAINS OF HRS, A PR... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1dvp | ||||||
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Title | CRYSTAL STRUCTURE OF THE VHS AND FYVE TANDEM DOMAINS OF HRS, A PROTEIN INVOLVED IN MEMBRANE TRAFFICKING AND SIGNAL TRANSDUCTION | ||||||
Components | HEPATOCYTE GROWTH FACTOR-REGULATED TYROSINE KINASE SUBSTRATE | ||||||
Keywords | TRANSFERASE / HRS / VHS / FYVE / Zinc finger / superhelix | ||||||
Function / homology | Function and homology information negative regulation of torso signaling pathway / Lysosome Vesicle Biogenesis / RHOBTB3 ATPase cycle / regulation of epithelial cell migration, open tracheal system / Endosomal Sorting Complex Required For Transport (ESCRT) / EGFR downregulation / Cargo recognition for clathrin-mediated endocytosis / positive regulation of Toll signaling pathway / Clathrin-mediated endocytosis / Ub-specific processing proteases ...negative regulation of torso signaling pathway / Lysosome Vesicle Biogenesis / RHOBTB3 ATPase cycle / regulation of epithelial cell migration, open tracheal system / Endosomal Sorting Complex Required For Transport (ESCRT) / EGFR downregulation / Cargo recognition for clathrin-mediated endocytosis / positive regulation of Toll signaling pathway / Clathrin-mediated endocytosis / Ub-specific processing proteases / positive regulation of Wnt protein secretion / positive regulation of border follicle cell migration / exosomal secretion / endosome transport via multivesicular body sorting pathway / early endosome to late endosome transport / endocytic recycling / endosomal transport / neuron remodeling / negative regulation of smoothened signaling pathway / ubiquitin binding / intracellular protein transport / receptor internalization / endocytosis / cell cortex / regulation of cell cycle / endosome / perinuclear region of cytoplasm / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2 Å | ||||||
Authors | Mao, Y. / Nickitenko, A. / Duan, X. / Lloyd, T.E. / Wu, M.N. / Bellen, H. / Quiocho, F.A. | ||||||
Citation | Journal: Cell(Cambridge,Mass.) / Year: 2000 Title: Crystal structure of the VHS and FYVE tandem domains of Hrs, a protein involved in membrane trafficking and signal transduction. Authors: Mao, Y. / Nickitenko, A. / Duan, X. / Lloyd, T.E. / Wu, M.N. / Bellen, H. / Quiocho, F.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1dvp.cif.gz | 60.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1dvp.ent.gz | 44 KB | Display | PDB format |
PDBx/mmJSON format | 1dvp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dv/1dvp ftp://data.pdbj.org/pub/pdb/validation_reports/dv/1dvp | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 25115.869 Da / Num. of mol.: 1 / Fragment: N-TERMINAL VHS AND FYVE TANDEM DOMAINS Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Plasmid: PTYB1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q960X8 | ||||
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#2: Chemical | #3: Chemical | ChemComp-CIT / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.37 Å3/Da / Density % sol: 63.5 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: PEG10000, Sodium Citrate, Hepes, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 5.5 / Method: vapor diffusion | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X4A / Wavelength: 0.9717 |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jul 8, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9717 Å / Relative weight: 1 |
Reflection | Resolution: 2→100 Å / Num. all: 22957 / Num. obs: 22397 / % possible obs: 99.8 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 3.9 % / Biso Wilson estimate: 15.1 Å2 / Rmerge(I) obs: 0.069 / Net I/σ(I): 15 |
Reflection shell | Resolution: 2→2.07 Å / Redundancy: 4 % / Rmerge(I) obs: 0.29 / Num. unique all: 2261 / % possible all: 100 |
Reflection | *PLUS Num. obs: 22957 / Num. measured all: 89859 |
Reflection shell | *PLUS % possible obs: 100 % |
-Processing
Software |
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Refinement | Resolution: 2→50 Å / σ(F): 2 / σ(I): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2→50 Å
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Refine LS restraints |
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