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Yorodumi- PDB-1dsv: STRUCTURE OF THE MMTV NUCLEOCAPSID PROTEIN (C-TERMINAL ZINC FINGER) -
+Open data
-Basic information
Entry | Database: PDB / ID: 1dsv | ||||||
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Title | STRUCTURE OF THE MMTV NUCLEOCAPSID PROTEIN (C-TERMINAL ZINC FINGER) | ||||||
Components | NUCLEIC ACID BINDING PROTEIN P14 | ||||||
Keywords | VIRAL PROTEIN / CCHC type zinc finger / VIRUS/VIRAL PROTEIN | ||||||
Function / homology | Function and homology information viral budding via host ESCRT complex / viral nucleocapsid / structural constituent of virion / nucleotide binding / DNA binding / zinc ion binding Similarity search - Function | ||||||
Biological species | Mouse mammary tumor virus | ||||||
Method | SOLUTION NMR | ||||||
Authors | Klein, D.J. / Johnson, P.E. / Zollars, E.S. / De Guzman, R.N. / Summers, M.F. | ||||||
Citation | Journal: Biochemistry / Year: 2000 Title: The NMR structure of the nucleocapsid protein from the mouse mammary tumor virus reveals unusual folding of the C-terminal zinc knuckle. Authors: Klein, D.J. / Johnson, P.E. / Zollars, E.S. / De Guzman, R.N. / Summers, M.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1dsv.cif.gz | 196.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1dsv.ent.gz | 161.8 KB | Display | PDB format |
PDBx/mmJSON format | 1dsv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1dsv_validation.pdf.gz | 338.4 KB | Display | wwPDB validaton report |
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Full document | 1dsv_full_validation.pdf.gz | 463.7 KB | Display | |
Data in XML | 1dsv_validation.xml.gz | 12.4 KB | Display | |
Data in CIF | 1dsv_validation.cif.gz | 19.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ds/1dsv ftp://data.pdbj.org/pub/pdb/validation_reports/ds/1dsv | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 3522.127 Da / Num. of mol.: 1 / Fragment: C-TERMINAL ZINC FINGER OF NUCLEOCAPSID PROTEIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mouse mammary tumor virus / Genus: Betaretrovirus / Plasmid: PET-3D / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)PLYSE / References: UniProt: P11284 |
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#2: Chemical | ChemComp-ZN / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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-Sample preparation
Details | Contents: 1 mM unlabelled MMTV Nc, 25 mM acetate (d3), pH 7.0, 20 mM NaCl, 0.1 mM ZnCl2, 0.1 mM BME Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: ~50 mM / pH: 7.0 / Pressure: ambient / Temperature: 25 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Software ordinal: 1 Details: data was collected on the intact nucleocapsid protein but the structures of the N- and C-terminal zinc fingers were calculated independently. These two domains do not interact with each other | ||||||||||||||||
NMR representative | Selection criteria: lowest target function | ||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |