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Yorodumi- PDB-1dj2: STRUCTURES OF ADENYLOSUCCINATE SYNTHETASE FROM TRITICUM AESTIVUM ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1dj2 | ||||||
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Title | STRUCTURES OF ADENYLOSUCCINATE SYNTHETASE FROM TRITICUM AESTIVUM AND ARABIDOPSIS THALIANA | ||||||
Components | ADENYLOSUCCINATE SYNTHETASEAdenylosuccinate synthase | ||||||
Keywords | LIGASE / GDP | ||||||
Function / homology | Function and homology information adenylosuccinate synthase / adenylosuccinate synthase activity / IMP metabolic process / 'de novo' AMP biosynthetic process / apoplast / plastid / chloroplast stroma / response to cadmium ion / chloroplast / GTP binding ...adenylosuccinate synthase / adenylosuccinate synthase activity / IMP metabolic process / 'de novo' AMP biosynthetic process / apoplast / plastid / chloroplast stroma / response to cadmium ion / chloroplast / GTP binding / magnesium ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.9 Å | ||||||
Authors | Prade, L. / Cowan-Jacob, S.W. / Chemla, P. / Potter, S. / Ward, E. / Fonne-Pfister, R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2000 Title: Structures of adenylosuccinate synthetase from Triticum aestivum and Arabidopsis thaliana. Authors: Prade, L. / Cowan-Jacob, S.W. / Chemla, P. / Potter, S. / Ward, E. / Fonne-Pfister, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1dj2.cif.gz | 169.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1dj2.ent.gz | 136.2 KB | Display | PDB format |
PDBx/mmJSON format | 1dj2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dj/1dj2 ftp://data.pdbj.org/pub/pdb/validation_reports/dj/1dj2 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 47886.250 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Cellular location (production host): CYTOSOL / Production host: Escherichia coli (E. coli) / References: UniProt: Q96529, adenylosuccinate synthase #2: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.19 Å3/Da / Density % sol: 70.63 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 4.5 Details: PEG 4000, Lithium sulfate, citrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7 | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 113 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: BM1A / Wavelength: 1 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Feb 28, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→45 Å / Num. all: 33374 / Num. obs: 33374 / % possible obs: 93.8 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 3.3 % / Biso Wilson estimate: 14.7 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 11.4 |
Reflection shell | Resolution: 2.9→3 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.433 / % possible all: 87.9 |
Reflection | *PLUS |
-Processing
Software |
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Refinement | Resolution: 2.9→45 Å / σ(F): 2 / σ(I): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.9→45 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.851 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.9 Å / Lowest resolution: 45 Å / σ(F): 2 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |