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Yorodumi- PDB-1dj3: STRUCTURES OF ADENYLOSUCCINATE SYNTHETASE FROM TRITICUM AESTIVUM ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1dj3 | ||||||
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Title | STRUCTURES OF ADENYLOSUCCINATE SYNTHETASE FROM TRITICUM AESTIVUM AND ARABIDOPSIS THALIANA | ||||||
Components | ADENYLOSUCCINATE SYNTHETASEAdenylosuccinate synthase | ||||||
Keywords | LIGASE / GDP | ||||||
Function / homology | Function and homology information adenylosuccinate synthase / adenylosuccinate synthase activity / IMP metabolic process / 'de novo' AMP biosynthetic process / chloroplast / GTP binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Triticum aestivum (bread wheat) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 3 Å | ||||||
Authors | Prade, L. / Cowan-Jacob, S.W. / Chemla, P. / Potter, S. / Ward, E. / Fonne-Pfister, R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2000 Title: Structures of adenylosuccinate synthetase from Triticum aestivum and Arabidopsis thaliana. Authors: Prade, L. / Cowan-Jacob, S.W. / Chemla, P. / Potter, S. / Ward, E. / Fonne-Pfister, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1dj3.cif.gz | 172.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1dj3.ent.gz | 138.8 KB | Display | PDB format |
PDBx/mmJSON format | 1dj3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dj/1dj3 ftp://data.pdbj.org/pub/pdb/validation_reports/dj/1dj3 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 47793.129 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Triticum aestivum (bread wheat) / Cellular location (production host): CYTOSOL / Production host: Escherichia coli (E. coli) / References: UniProt: O24396, adenylosuccinate synthase #2: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.76 Å3/Da / Density % sol: 55.38 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: PEG 4000, lithium sulfate, citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7 | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: BM1A / Wavelength: 1 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Nov 11, 1997 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3→33 Å / Num. all: 20426 / Num. obs: 20426 / % possible obs: 84.1 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 3.1 % / Biso Wilson estimate: 14.7 Å2 / Rmerge(I) obs: 0.099 / Net I/σ(I): 11.4 |
Reflection shell | Resolution: 3→3.1 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.338 / % possible all: 98.4 |
Reflection | *PLUS |
-Processing
Software |
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Refinement | Resolution: 3→10 Å / σ(F): 2 / σ(I): 2 / Stereochemistry target values: Engh and Huber / Details: NCS restraints were set.
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Refinement step | Cycle: LAST / Resolution: 3→10 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.851 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Highest resolution: 3 Å / Lowest resolution: 10 Å / σ(F): 2 / Rfactor obs: 0.24 / Rfactor Rwork: 0.24 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |