[English] 日本語
Yorodumi- PDB-1dis: DIHYDROFOLATE REDUCTASE (E.C.1.5.1.3) COMPLEX WITH BRODIMOPRIM-4,... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1dis | ||||||
---|---|---|---|---|---|---|---|
Title | DIHYDROFOLATE REDUCTASE (E.C.1.5.1.3) COMPLEX WITH BRODIMOPRIM-4,6-DICARBOXYLATE | ||||||
Components | DIHYDROFOLATE REDUCTASE | ||||||
Keywords | OXIDOREDUCTASE / INHIBITOR-ENZYME COMPLEX / OXIDO-REDUCTASE | ||||||
Function / homology | Function and homology information response to methotrexate / glycine biosynthetic process / dihydrofolate reductase / dihydrofolate reductase activity / tetrahydrofolate biosynthetic process / one-carbon metabolic process / NADP binding / response to antibiotic Similarity search - Function | ||||||
Biological species | Lactobacillus casei (bacteria) | ||||||
Method | SOLUTION NMR | ||||||
Authors | Morgan, W.D. / Birdsall, B. / Polshakov, V.I. / Sali, D. / Kompis, I. / Feeney, J. | ||||||
Citation | Journal: Biochemistry / Year: 1995 Title: Solution structure of a brodimoprim analogue in its complex with Lactobacillus casei dihydrofolate reductase. Authors: Morgan, W.D. / Birdsall, B. / Polshakov, V.I. / Sali, D. / Kompis, I. / Feeney, J. #1: Journal: Biochemistry / Year: 1994 Title: Solution Structure of Bound Trimethoprim in its Complex with Lactobacillus Casei Dihydrofolate Reductase Authors: Martorell, G. / Gradwell, M.J. / Birdsall, B. / Bauer, C.J. / Frenkiel, T.A. / Cheung, H.T.A. / Polshakov, V.I. / Kuyper, L. / Feeney, J. #2: Journal: J.Med.Chem. / Year: 1984 Title: A 1H NMR Study of the Interactions and Conformations of Rationally Designed Brodimoprim Analogues in Complexes with Lactobacillus Casei Dihydrofolate Reductase Authors: Birdsall, B. / Feeney, J. / Pascual, C. / Roberts, G.C.K. / Kompis, I. / Then, R.L. / Mueller, K. / Kroehn, A. #3: Journal: J.Biol.Chem. / Year: 1982 Title: Crystal Structures of Escherichia Coli and Lactobacillus Casei Dihydrofolate Reductase Refined at 1.7 Angstroms Resolution. I. General Features and Binding of Methotrexate Authors: Bolin, J.T. / Filman, D.J. / Matthews, D.A. / Hamlin, R.C. / Kraut, J. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1dis.cif.gz | 59.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1dis.ent.gz | 48 KB | Display | PDB format |
PDBx/mmJSON format | 1dis.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/di/1dis ftp://data.pdbj.org/pub/pdb/validation_reports/di/1dis | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
Atom site foot note | 1: CIS PROLINE - PRO 53 2: GLY 98 - GLY 99 OMEGA = 7.98 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION | |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 18331.594 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Lactobacillus casei (bacteria) / Strain: METHOTREXATE-RESISTANT / Gene: POTENTIAL / Production host: Escherichia coli (E. coli) / References: UniProt: P00381, dihydrofolate reductase |
---|---|
#2: Chemical | ChemComp-BDM / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
---|
-Sample preparation
Crystal grow | *PLUS Method: other |
---|
-Processing
NMR software | Name: Discover / Classification: refinement |
---|---|
NMR ensemble | Conformers submitted total number: 1 |