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Open data
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Basic information
Entry | Database: PDB / ID: 1dco | ||||||
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Title | DCOH, A BIFUNCTIONAL PROTEIN-BINDING TRANSCRIPTIONAL COACTIVATOR | ||||||
![]() | DCOH | ||||||
![]() | TRANSCRIPTIONAL STIMULATOR / DIMERIZATION COFACTOR / DEHYDRATASE / 4A-CARBINOLAMINE DEHYDRATASE / TRANSREGULATOR OF HOMEODOMAIN PROTEINS | ||||||
Function / homology | ![]() Phenylalanine metabolism / 4a-hydroxytetrahydrobiopterin dehydratase / 4-alpha-hydroxytetrahydrobiopterin dehydratase activity / L-phenylalanine metabolic process / phenylalanine 4-monooxygenase activity / regulation of protein binding / tetrahydrobiopterin biosynthetic process / transcription coactivator activity / positive regulation of DNA-templated transcription / identical protein binding ...Phenylalanine metabolism / 4a-hydroxytetrahydrobiopterin dehydratase / 4-alpha-hydroxytetrahydrobiopterin dehydratase activity / L-phenylalanine metabolic process / phenylalanine 4-monooxygenase activity / regulation of protein binding / tetrahydrobiopterin biosynthetic process / transcription coactivator activity / positive regulation of DNA-templated transcription / identical protein binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Cronk, J.D. / Endrizzi, J.A. / Alber, T. | ||||||
![]() | ![]() Title: High-resolution structures of the bifunctional enzyme and transcriptional coactivator DCoH and its complex with a product analogue. Authors: Cronk, J.D. / Endrizzi, J.A. / Alber, T. #1: ![]() Title: Crystal Structure of DCoH, a Bifunctional, Protein-Binding Transcriptional Coactivator Authors: Endrizzi, J.A. / Cronk, J.D. / Wang, W. / Crabtree, G.R. / Alber, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 169.1 KB | Display | ![]() |
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PDB format | ![]() | 137.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 416.9 KB | Display | ![]() |
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Full document | ![]() | 475.2 KB | Display | |
Data in XML | ![]() | 22.5 KB | Display | |
Data in CIF | ![]() | 35.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 12017.603 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P61459, 4a-hydroxytetrahydrobiopterin dehydratase #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.29 Å3/Da / Density % sol: 65 % |
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Crystal grow | pH: 7.4 / Details: ROOM TEMPERATURE, 1.7M AMSO4, 0.1M HEPES, PH 7.4 / Temp details: room temp |
-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Sep 20, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→20 Å / Num. obs: 44232 / % possible obs: 75.6 % / Observed criterion σ(I): 0 / Redundancy: 7 % / Rmerge(I) obs: 0.072 |
Reflection | *PLUS Num. obs: 43794 / % possible obs: 75 % / Num. measured all: 310522 |
Reflection shell | *PLUS Highest resolution: 2.3 Å / Lowest resolution: 2.45 Å / % possible obs: 33 % / Mean I/σ(I) obs: 2 |
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Processing
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Refinement | Resolution: 2.3→20 Å / σ(F): 0 / Stereochemistry target values: TNT RESTRAINTS Details: CORRELATED B-VALUE REFINEMENT WAS CARRIED OUT WITH TNT. MEAN B VALUE 14.7 ANGSTROMS**2 FINAL RMS COORD. SHIFT 0.01 ANGSTROMS
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Refinement step | Cycle: LAST / Resolution: 2.3→20 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.175 | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 14.7 Å2 | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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