IgM B cell receptor complex / B cell receptor complex / CD22 mediated BCR regulation / B cell activation / B cell proliferation / multivesicular body / B cell differentiation / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / B cell receptor signaling pathway / transmembrane signaling receptor activity ...IgM B cell receptor complex / B cell receptor complex / CD22 mediated BCR regulation / B cell activation / B cell proliferation / multivesicular body / B cell differentiation / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / B cell receptor signaling pathway / transmembrane signaling receptor activity / adaptive immune response / Potential therapeutics for SARS / membrane raft / external side of plasma membrane / identical protein binding / plasma membrane Similarity search - Function
SOLUTION NMR / THE SIMULATED ANNEALING PROTOCOL IN THE X-PLOR 3.1 MANUAL WAS USED FOR STRUCTURE CALCULATION. RESTRAINED POWELL MINIMIZATION INCOPORATED THE CHARMM FORCE FIELD USING THE TOP_ALL22, PAR_ALL22 FILES IN THE X-PLOR 3.1 LIBRARY. 107 NOE DERIVED DISTANCE RESTRAINTS WERE USED THROUGH OUT THE STRUCTURE CALCULATION
Mass: 1433.456 Da / Num. of mol.: 1 / Fragment: RESIDUES 178-189 / Source method: obtained synthetically / Details: SEQUENCE FROM HUMAN IG-ALPHA ITAM / References: UniProt: P11912*PLUS
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Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D NOESY
1
2
1
2D TOCSY
1
3
1
2D ROESY
NMR details
Text: THIS STRUCTURE WAS DETERMINED USING STANDARD 2D HOMONUCLEAR TECHNIQUES
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Sample preparation
Details
Contents: 1 MM ITP WITH 0.2 MM KLYN, PH 7.0 PHOSPHATE BUFFER
Sample conditions
pH: 7.0 / Pressure: AMBIENT / Temperature: 278 K
Crystal grow
*PLUS
Method: other / Details: NMR
-
NMR measurement
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Varian UNITY
Varian
UNITY
600
1
Varian UNITY
Varian
UNITY
500
2
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Processing
NMR software
Name
Version
Developer
Classification
X-PLOR
3.1
BRUNGER, A.T.
structuresolution
CHARMM
23.2
BROOKS, B.R. ETAL.
refinement
Refinement
Method: THE SIMULATED ANNEALING PROTOCOL IN THE X-PLOR 3.1 MANUAL WAS USED FOR STRUCTURE CALCULATION. RESTRAINED POWELL MINIMIZATION INCOPORATED THE CHARMM FORCE FIELD USING THE TOP_ALL22, PAR_ALL22 ...Method: THE SIMULATED ANNEALING PROTOCOL IN THE X-PLOR 3.1 MANUAL WAS USED FOR STRUCTURE CALCULATION. RESTRAINED POWELL MINIMIZATION INCOPORATED THE CHARMM FORCE FIELD USING THE TOP_ALL22, PAR_ALL22 FILES IN THE X-PLOR 3.1 LIBRARY. 107 NOE DERIVED DISTANCE RESTRAINTS WERE USED THROUGH OUT THE STRUCTURE CALCULATION Software ordinal: 1
NMR representative
Selection criteria: closest to the average
NMR ensemble
Conformer selection criteria: structures with favorable non-bond energy Conformers calculated total number: 200 / Conformers submitted total number: 1
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