+Open data
-Basic information
Entry | Database: PDB / ID: 2l5m | ||||||
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Title | Solution structure of GF-17 in complex with micelles | ||||||
Components | GF-17 | ||||||
Keywords | ANTIMICROBIAL PROTEIN / Antimicrobial peptide / LL-37-derived | ||||||
Biological species | artificial gene (others) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Wang, G. | ||||||
Citation | Journal: Antimicrob.Agents Chemother. / Year: 2012 Title: Decoding the Functional Roles of Cationic Side Chains of the Major Antimicrobial Region of Human Cathelicidin LL-37. Authors: Wang, G. / Epand, R.F. / Mishra, B. / Lushnikova, T. / Thomas, V.C. / Bayles, K.W. / Epand, R.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2l5m.cif.gz | 108.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2l5m.ent.gz | 85 KB | Display | PDB format |
PDBx/mmJSON format | 2l5m.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l5/2l5m ftp://data.pdbj.org/pub/pdb/validation_reports/l5/2l5m | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2105.575 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) artificial gene (others) |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 2 mM GF-17-1, 80 mM [U-99% 2H] SDS-2, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||
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Sample |
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Sample conditions | Ionic strength: 0.08 / pH: 5.4 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: closest to the average | |||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |