+Open data
-Basic information
Entry | Database: PDB / ID: 1csn | ||||||
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Title | BINARY COMPLEX OF CASEIN KINASE-1 WITH MGATP | ||||||
Components | CASEIN KINASE-1 | ||||||
Keywords | PHOSPHOTRANSFERASE | ||||||
Function / homology | Function and homology information fungal-type vacuole / regulation of endocytosis / endocytosis / protein tyrosine kinase activity / non-specific serine/threonine protein kinase / protein serine kinase activity / protein serine/threonine kinase activity / magnesium ion binding / signal transduction / ATP binding ...fungal-type vacuole / regulation of endocytosis / endocytosis / protein tyrosine kinase activity / non-specific serine/threonine protein kinase / protein serine kinase activity / protein serine/threonine kinase activity / magnesium ion binding / signal transduction / ATP binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Schizosaccharomyces pombe (fission yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2 Å | ||||||
Authors | Xu, R.-M. / Cheng, X. | ||||||
Citation | Journal: EMBO J. / Year: 1995 Title: Crystal structure of casein kinase-1, a phosphate-directed protein kinase. Authors: Xu, R.M. / Carmel, G. / Sweet, R.M. / Kuret, J. / Cheng, X. #1: Journal: J.Biol.Chem. / Year: 1994 Title: Expression, Purification, Crystallization, and Preliminary X-Ray Analysis of Casein Kinase-1 from Schizosaccharomyces Pombe Authors: Carmel, G. / Leichus, B. / Cheng, X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1csn.cif.gz | 74.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1csn.ent.gz | 55.1 KB | Display | PDB format |
PDBx/mmJSON format | 1csn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1csn_validation.pdf.gz | 466.4 KB | Display | wwPDB validaton report |
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Full document | 1csn_full_validation.pdf.gz | 470 KB | Display | |
Data in XML | 1csn_validation.xml.gz | 7.9 KB | Display | |
Data in CIF | 1csn_validation.cif.gz | 11.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cs/1csn ftp://data.pdbj.org/pub/pdb/validation_reports/cs/1csn | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 34377.238 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Schizosaccharomyces pombe (fission yeast) References: UniProt: P40233, Transferases; Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor | ||||
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#2: Chemical | ChemComp-MG / | ||||
#3: Chemical | #4: Chemical | ChemComp-ATP / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.15 Å3/Da / Density % sol: 60.95 % Description: THE WAVELENGTH OF 1.0 WAS USED FOR THE NATIVE DATA SETS AND 1.0012 FOR THE HG DERIVATIVE DATA SETS. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 16 ℃ / pH: 7 / Method: vapor diffusion, hanging drop / Details: Carmel, G., (1994) J.Biol.Chem., 269, 7304. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X12C / Wavelength: 1.0, 1.0012 | |||||||||
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Detector | Detector: AREA DETECTOR | |||||||||
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Num. obs: 24245 / Redundancy: 6.7 % / Rmerge(I) obs: 0.0804 | |||||||||
Reflection | *PLUS Highest resolution: 2 Å / Lowest resolution: 20 Å / % possible obs: 93.9 % / Num. measured all: 161782 / Rmerge(I) obs: 0.0804 | |||||||||
Reflection shell | *PLUS Highest resolution: 2 Å / Lowest resolution: 2.09 Å / % possible obs: 44.2 % |
-Processing
Software |
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Refinement | Resolution: 2→11.3 Å /
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Refinement step | Cycle: LAST / Resolution: 2→11.3 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |