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- PDB-1c8a: NMR STRUCTURE OF INTRAMOLECULAR DIMER ANTIFREEZE PROTEIN RD3, 40 ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1c8a | ||||||
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Title | NMR STRUCTURE OF INTRAMOLECULAR DIMER ANTIFREEZE PROTEIN RD3, 40 SA STRUCTURES | ||||||
![]() | PROTEIN (ANTIFREEZE PROTEIN TYPE III) | ||||||
![]() | ANTIFREEZE PROTEIN / ANTIFREEZE / THERMAL HYSTERESIS PROTEIN / ICE BINDING PROTEIN | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / SA | ||||||
![]() | Miura, K. / Tsuda, S. | ||||||
![]() | ![]() Title: NMR analysis of type III antifreeze protein intramolecular dimer. Structural basis for enhanced activity. Authors: Miura, K. / Ohgiya, S. / Hoshino, T. / Nemoto, N. / Suetake, T. / Miura, A. / Spyracopoulos, L. / Kondo, H. / Tsuda, S. #1: ![]() Title: Determination of the Solution Structure of the N-Domain Plus Linker of Antarctic Eel Pout Antifreeze Protein Rd3 Authors: Miura, K. / Ohgiya, S. / Hoshino, T. / Nemoto, N. / Odaira, M. / Nitta, K. / Tsuda, S. #2: ![]() Title: Structural Basis for the Binding of a Globular Antifreeze Protein to Ice Authors: Jia, Z. / Deluca, C.I. / Chao, H. / Davies, P.L. #3: ![]() Title: Refined Solution Structure of Type III Antifreeze Protein: Hydrophobic Groups May be Involved in the Energetics of the Protein-Ice Interaction Authors: Sonnichsen, F.D. / Deluca, C.I. / Davies, P.L. / Sykes, B.D. #4: ![]() Title: Antifreeze Peptide Heterogeneity in an Antarctic Eel Pout Includes an Unusually Large Major Variant Comprised of Two 7 kDa Type III Afps Linked in Tandem Authors: Wang, X. / Devries, A.L. / Cheng, C.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 55.7 KB | Display | ![]() |
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PDB format | ![]() | 41.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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NMR ensembles |
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Components
#1: Protein | Mass: 14490.035 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Tissue: BLOOD PLASMA / Description: SYNTHETIC GENE / Variant: RD3 / Plasmid: PKK223-3UC / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C/15N-LABELED RD3 |
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Sample preparation
Details | Contents: 90% H2O/10% D2O |
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Sample conditions | pH: 6.7 / Pressure: 1 atm / Temperature: 277 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITY / Manufacturer: Varian / Model: UNITY / Field strength: 500 MHz |
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Processing
NMR software |
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Refinement | Method: SA / Software ordinal: 1 | ||||||||||||
NMR ensemble | Conformer selection criteria: AVERAGE / Conformers calculated total number: 40 / Conformers submitted total number: 1 |