+Open data
-Basic information
Entry | Database: PDB / ID: 1c3h | ||||||
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Title | ACRP30 CALCIUM COMPLEX | ||||||
Components | 30 KD ADIPOCYTE COMPLEMENT-RELATED PROTEIN PRECURSOR | ||||||
Keywords | SIGNALING PROTEIN / ACRP30 C1Q TNF TRIMER ALL-BETA CALCIUM-BOUND | ||||||
Function / homology | Function and homology information AMPK inhibits chREBP transcriptional activation activity / negative regulation of intracellular protein transport / positive regulation of lipid transporter activity / positive regulation of metanephric podocyte development / positive regulation of renal albumin absorption / negative regulation of metanephric mesenchymal cell migration / response to linoleic acid / positive regulation of glycogen (starch) synthase activity / negative regulation of platelet-derived growth factor receptor-alpha signaling pathway / positive regulation of myeloid cell apoptotic process ...AMPK inhibits chREBP transcriptional activation activity / negative regulation of intracellular protein transport / positive regulation of lipid transporter activity / positive regulation of metanephric podocyte development / positive regulation of renal albumin absorption / negative regulation of metanephric mesenchymal cell migration / response to linoleic acid / positive regulation of glycogen (starch) synthase activity / negative regulation of platelet-derived growth factor receptor-alpha signaling pathway / positive regulation of myeloid cell apoptotic process / negative regulation of macrophage differentiation / detection of oxidative stress / positive regulation of signal transduction / negative regulation of hormone secretion / low-density lipoprotein particle clearance / negative regulation of protein autophosphorylation / negative regulation of granulocyte differentiation / response to sucrose / sialic acid binding / negative regulation of heterotypic cell-cell adhesion / negative regulation of vascular associated smooth muscle cell migration / collagen trimer / negative regulation of DNA biosynthetic process / negative regulation of platelet-derived growth factor receptor signaling pathway / positive regulation of monocyte chemotactic protein-1 production / negative regulation of phagocytosis / negative regulation of synaptic transmission / positive regulation of cAMP-dependent protein kinase activity / negative regulation of cold-induced thermogenesis / negative regulation of fat cell differentiation / fatty acid beta-oxidation / positive regulation of fatty acid metabolic process / positive regulation of protein kinase A signaling / fatty acid oxidation / negative regulation of vascular associated smooth muscle cell proliferation / negative regulation of macrophage derived foam cell differentiation / negative regulation of tumor necrosis factor production / regulation of glucose metabolic process / positive regulation of cholesterol efflux / negative regulation of tumor necrosis factor-mediated signaling pathway / response to tumor necrosis factor / negative regulation of gluconeogenesis / response to glucose / brown fat cell differentiation / negative regulation of canonical NF-kappaB signal transduction / cellular response to cAMP / response to glucocorticoid / cellular response to epinephrine stimulus / negative regulation of blood pressure / protein serine/threonine kinase activator activity / response to nutrient / negative regulation of cell migration / response to activity / negative regulation of MAP kinase activity / negative regulation of receptor binding / cell periphery / positive regulation of interleukin-8 production / protein localization to plasma membrane / positive regulation of glucose import / response to bacterium / negative regulation of ERK1 and ERK2 cascade / hormone activity / negative regulation of inflammatory response / circadian rhythm / cellular response to insulin stimulus / glucose metabolic process / positive regulation of peptidyl-tyrosine phosphorylation / cellular response to xenobiotic stimulus / glucose homeostasis / positive regulation of cold-induced thermogenesis / gene expression / response to ethanol / positive regulation of canonical NF-kappaB signal transduction / response to hypoxia / positive regulation of protein phosphorylation / signaling receptor binding / negative regulation of DNA-templated transcription / perinuclear region of cytoplasm / cell surface / endoplasmic reticulum / protein homodimerization activity / protein-containing complex / extracellular space / identical protein binding Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.1 Å | ||||||
Authors | Shapiro, L. / Boggon, T. / Scherer, P. | ||||||
Citation | Journal: To Be Published Title: ACRP30 calcium complex Authors: Boggon, T. / Scherer, P. / Shapiro, L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1c3h.cif.gz | 184.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1c3h.ent.gz | 146.8 KB | Display | PDB format |
PDBx/mmJSON format | 1c3h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/c3/1c3h ftp://data.pdbj.org/pub/pdb/validation_reports/c3/1c3h | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 15860.657 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Production host: Escherichia coli (E. coli) / References: UniProt: Q60994 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.53 Å3/Da / Density % sol: 51.36 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 0.1M BIS-TRIS, 20MM CACL2, 2% PEG 4K, -- CRYO: 15% ETHYLENE GLYCOL, 19% SUCROSE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 4CK, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X4A / Wavelength: 1.77114 |
Detector | Type: RIGAKU RAXIS / Detector: IMAGE PLATE / Date: Jan 1, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.77114 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→20 Å / Num. all: 51096 / Num. obs: 51096 / % possible obs: 93.9 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 3 % / Biso Wilson estimate: 25.7 Å2 / Rmerge(I) obs: 0.058 / Net I/σ(I): 24.87 |
Reflection shell | Resolution: 2.1→2.23 Å / Redundancy: 2 % / Rmerge(I) obs: 0.115 / % possible all: 89.4 |
-Processing
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Refinement | Resolution: 2.1→20 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: ENGH & HUBER
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Refinement step | Cycle: LAST / Resolution: 2.1→20 Å
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Refine LS restraints |
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