+Open data
-Basic information
Entry | Database: PDB / ID: 1bxu | ||||||
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Title | OXIDIZED PLASTOCYANIN FROM SYNECHOCOCCUS SP. | ||||||
Components | PLASTOCYANIN | ||||||
Keywords | COPPER PROTEIN / ELECTRON TRANSFER | ||||||
Function / homology | Function and homology information plasma membrane-derived thylakoid membrane / electron transfer activity / copper ion binding Similarity search - Function | ||||||
Biological species | Synechococcus elongatus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Inoue, T. / Sugawara, H. / Hamanaka, S. / Tsukui, H. / Suzuki, E. / Kohzuma, T. / Kai, Y. | ||||||
Citation | Journal: Biochemistry / Year: 1999 Title: Crystal structure determinations of oxidized and reduced plastocyanin from the cyanobacterium Synechococcus sp. PCC 7942. Authors: Inoue, T. / Sugawara, H. / Hamanaka, S. / Tsukui, H. / Suzuki, E. / Kohzuma, T. / Kai, Y. #1: Journal: Acta Crystallogr.,Sect.D / Year: 1999 Title: Crystallization and Preliminary X-Ray Analysis of Plastocyanin from Cyanobacterium Synechococcus Sp. Pcc 7942 Authors: Inoue, T. / Sugawara, H. / Hamanaka, S. / Tsukui, H. / Suzuki, E. / Kohzuma, T. / Kai, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1bxu.cif.gz | 27.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1bxu.ent.gz | 19.8 KB | Display | PDB format |
PDBx/mmJSON format | 1bxu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bx/1bxu ftp://data.pdbj.org/pub/pdb/validation_reports/bx/1bxu | HTTPS FTP |
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-Related structure data
Related structure data | 1bxvC 1iuzS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 9861.011 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: REDUCED FORM / Source: (gene. exp.) Synechococcus elongatus (bacteria) / Strain: PCC 7942 / Production host: Escherichia coli (E. coli) / References: UniProt: P55020 |
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#2: Chemical | ChemComp-CU / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 54.5 % | |||||||||||||||
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Crystal grow | pH: 5.3 / Details: pH 5.3 | |||||||||||||||
Crystal grow | *PLUS Method: unknown | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 300 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: BL-6A / Wavelength: 1 |
Detector | Type: WEISSENBERG / Detector: DIFFRACTOMETER / Date: Dec 1, 1997 |
Radiation | Monochromator: SI(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→30 Å / Num. obs: 8058 / % possible obs: 99.1 % / Redundancy: 4.4 % / Rmerge(I) obs: 0.071 |
Reflection shell | Resolution: 1.9→1.97 Å / Rmerge(I) obs: 0.123 / % possible all: 97.3 |
Reflection | *PLUS Num. measured all: 39058 |
Reflection shell | *PLUS % possible obs: 97.3 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1IUZ Resolution: 1.9→10 Å / SU B: 2.46 / SU ML: 0.07 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.13 / ESU R Free: 0.12
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Displacement parameters | Biso mean: 17.7 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→10 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Num. reflection obs: 7192 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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