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Open data
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Basic information
Entry | Database: PDB / ID: 1bsl | ||||||
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Title | STRUCTURE OF ALKANAL MONOOXYGENASE BETA CHAIN | ||||||
![]() | BACTERIAL LUCIFERASE | ||||||
![]() | FLAVOPROTEIN / PHOTOPROTEIN / LUMINESCENCE / OXIDOREDUCTASE / MONOOXYGENASE / FMN | ||||||
Function / homology | ![]() bacterial luciferase / alkanal monooxygenase (FMN-linked) activity / bioluminescence / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Rayment, I. / Holden, H.M. / Thoden, J.B. / Baldwin, T.O. | ||||||
![]() | ![]() Title: Structure of the beta 2 homodimer of bacterial luciferase from Vibrio harveyi: X-ray analysis of a kinetic protein folding trap. Authors: Thoden, J.B. / Holden, H.M. / Fisher, A.J. / Sinclair, J.F. / Wesenberg, G. / Baldwin, T.O. / Rayment, I. #1: ![]() Title: The 1.5-A Resolution Crystal Structure of Bacterial Luciferase in Low Salt Conditions Authors: Fisher, A.J. / Thompson, T.B. / Thoden, J.B. / Baldwin, T.O. / Rayment, I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 160.9 KB | Display | ![]() |
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PDB format | ![]() | 126.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 379.5 KB | Display | ![]() |
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Full document | ![]() | 419.4 KB | Display | |
Data in XML | ![]() | 18.9 KB | Display | |
Data in CIF | ![]() | 33.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 36384.684 Da / Num. of mol.: 2 / Fragment: BETA SUBUNIT Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.73 Å3/Da / Density % sol: 54.98 % | |||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Jun 1, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 55442 / % possible obs: 93.7 % / Redundancy: 2.2 % / Rmerge(I) obs: 0.041 |
Reflection | *PLUS Highest resolution: 1.95 Å / Lowest resolution: 30 Å / Num. measured all: 125700 |
Reflection shell | *PLUS Highest resolution: 1.95 Å / % possible obs: 85.1 % / Num. unique obs: 6873 / Num. measured obs: 9563 / Rmerge(I) obs: 0.18 |
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Processing
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Refinement | Resolution: 1.95→30 Å /
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Refinement step | Cycle: LAST / Resolution: 1.95→30 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor all: 0.188 | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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