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- PDB-1blk: NMR ENSEMBLE OF BLK SH2 DOMAIN USING CHEMICAL SHIFT REFINEMENT, 2... -

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Basic information

Entry
Database: PDB / ID: 1blk
TitleNMR ENSEMBLE OF BLK SH2 DOMAIN USING CHEMICAL SHIFT REFINEMENT, 20 STRUCTURES
ComponentsP55 BLK PROTEIN TYROSINE KINASE
KeywordsPHOSPHORYLATION / SIGNAL TRANSDUCTION / TYROSINE KINASE / TRANSFERASE / PHOSPHOTRANSFERASE
Function / homology
Function and homology information


cell surface receptor protein tyrosine kinase signaling pathway / B cell receptor signaling pathway / non-specific protein-tyrosine kinase / non-membrane spanning protein tyrosine kinase activity / positive regulation of insulin secretion / peptidyl-tyrosine phosphorylation / protein tyrosine kinase activity / cell differentiation / innate immune response / signaling receptor binding ...cell surface receptor protein tyrosine kinase signaling pathway / B cell receptor signaling pathway / non-specific protein-tyrosine kinase / non-membrane spanning protein tyrosine kinase activity / positive regulation of insulin secretion / peptidyl-tyrosine phosphorylation / protein tyrosine kinase activity / cell differentiation / innate immune response / signaling receptor binding / ATP binding / plasma membrane / cytosol
Similarity search - Function
Tyrosine-protein kinase Blk, SH2 domain / SH2 domain / SHC Adaptor Protein / : / SH3 domain / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / Src homology 3 domains ...Tyrosine-protein kinase Blk, SH2 domain / SH2 domain / SHC Adaptor Protein / : / SH3 domain / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / Src homology 3 domains / SH2 domain superfamily / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Tyrosine-protein kinase Blk
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodSOLUTION NMR
AuthorsMetzler, W.J. / Leiting, B. / Pryor, K. / Mueller, L. / Farmer II, B.T.
CitationJournal: Biochemistry / Year: 1996
Title: The three-dimensional solution structure of the SH2 domain from p55blk kinase.
Authors: Metzler, W.J. / Leiting, B. / Pryor, K. / Mueller, L. / Farmer 2nd., B.T.
History
DepositionMar 26, 1996Processing site: BNL
Revision 1.0Mar 12, 1997Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 16, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site / _struct_ref_seq_dif.details
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
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Assembly

Deposited unit
A: P55 BLK PROTEIN TYROSINE KINASE


Theoretical massNumber of molelcules
Total (without water)12,7511
Polymers12,7511
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / -
Representative

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Components

#1: Protein P55 BLK PROTEIN TYROSINE KINASE


Mass: 12750.652 Da / Num. of mol.: 1 / Fragment: SH2 DOMAIN, SRC HOMOLOGY 2
Source method: isolated from a genetically manipulated source
Details: PEPTIDE FREE / Source: (gene. exp.) Mus musculus (house mouse) / Gene: P55 BLK KINASE (RESIDUES 107 - / Plasmid: PGEX-2T / Species (production host): Escherichia coli / Gene (production host): P55 BLK KINASE (RESIDUES 107 - 218) / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P16277, EC: 2.7.1.112

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Crystal grow
*PLUS
Method: other / Details: NMR

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Processing

Software
NameClassification
X-PLORmodel building
X-PLORrefinement
X-PLORphasing
NMR softwareName: X-PLOR / Developer: BRUNGER / Classification: refinement
NMR ensembleConformers submitted total number: 20

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