+
Open data
-
Basic information
Entry | Database: PDB / ID: 1bk6 | ||||||
---|---|---|---|---|---|---|---|
Title | KARYOPHERIN ALPHA (YEAST) + SV40 T ANTIGEN NLS | ||||||
![]() |
| ||||||
![]() | COMPLEX (PROTEIN TRANSPORT/PEPTIDE) / PROTEIN TRANSPORT / NLS NUCLEAR IMPORT / ARMADILLO REPEAT CONTAINING PROTEIN / COMPLEX (PROTEIN TRANSPORT-PEPTIDE) / COMPLEX (PROTEIN TRANSPORT-PEPTIDE) complex | ||||||
Function / homology | ![]() proteasome localization / import into nucleus / NLS-dependent protein nuclear import complex / protein targeting to membrane / nuclear import signal receptor activity / nuclear localization sequence binding / NLS-bearing protein import into nucleus / protein import into nucleus / disordered domain specific binding / nuclear envelope ...proteasome localization / import into nucleus / NLS-dependent protein nuclear import complex / protein targeting to membrane / nuclear import signal receptor activity / nuclear localization sequence binding / NLS-bearing protein import into nucleus / protein import into nucleus / disordered domain specific binding / nuclear envelope / protein-containing complex binding / perinuclear region of cytoplasm / protein-containing complex / nucleoplasm / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Conti, E. / Uy, M. / Leighton, L. / Blobel, G. / Kuriyan, J. | ||||||
![]() | ![]() Title: Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha. Authors: Conti, E. / Uy, M. / Leighton, L. / Blobel, G. / Kuriyan, J. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 166.4 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 132 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 385.6 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 403.5 KB | Display | |
Data in XML | ![]() | 19.1 KB | Display | |
Data in CIF | ![]() | 28.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 46718.426 Da / Num. of mol.: 2 / Fragment: ARMADILLO DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: W303 / Cell line: BL21 / Cellular location: CYTOPLASMIC/NUCLEAR / Gene: SRP1 / Plasmid: PPROEX-HTB / Species (production host): Escherichia coli / Production host: ![]() ![]() #2: Protein/peptide | Mass: 791.059 Da / Num. of mol.: 2 / Fragment: NLS (NUCLEAR LOCALIZATION SIGNAL) Source method: isolated from a genetically manipulated source Details: NLS PEPTIDE AT THE LARGER (FUNCTIONAL) BINDING SITE #3: Protein/peptide | Mass: 489.608 Da / Num. of mol.: 2 / Fragment: NLS (NUCLEAR LOCALIZATION SIGNAL) Source method: isolated from a genetically manipulated source Details: NLS PEPTIDE AT THE SMALLER BINDING SITE #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 50 % | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | pH: 7 / Details: pH 7.0 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: BRANDEIS / Detector: CCD / Date: Dec 1, 1997 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→29.1 Å / Num. obs: 22717 / % possible obs: 86.9 % / Redundancy: 3.5 % / Rsym value: 0.064 / Net I/σ(I): 17.4 |
Reflection shell | Resolution: 2.8→2.9 Å / Redundancy: 2.4 % / Mean I/σ(I) obs: 9.9 / Rsym value: 0.188 / % possible all: 82.2 |
Reflection | *PLUS Num. measured all: 270860 / Rmerge(I) obs: 0.064 |
Reflection shell | *PLUS % possible obs: 82.2 % / Rmerge(I) obs: 0.188 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: DIFFERENCE FOURIER Starting model: UNLIGANDED KARYOPHERIN ALPHA Resolution: 2.8→29.1 Å / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Solvent model: FLAT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→29.1 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.8→2.85 Å / Total num. of bins used: 18
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: CNS / Version: 0.2 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | *PLUS Rfactor obs: 0.296 |