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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1bi8 | ||||||
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タイトル | MECHANISM OF G1 CYCLIN DEPENDENT KINASE INHIBITION FROM THE STRUCTURES CDK6-P19INK4D INHIBITOR COMPLEX | ||||||
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![]() | COMPLEX (KINASE/INHIBITOR) / CYCLIN DEPENDENT KINASE / CYCLIN DEPENDENT KINASE INHIBITORY PROTEIN / CDK / INK4 / CELL CYCLE / COMPLEX (KINASE-INHIBITOR) / COMPLEX (KINASE-INHIBITOR) complex | ||||||
機能・相同性 | ![]() cyclin D2-CDK6 complex / cyclin D2-CDK4 complex / cell dedifferentiation / Drug-mediated inhibition of CDK4/CDK6 activity / Evasion of Oncogene Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / Evasion of Oxidative Stress Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / FBXO family protein binding / autophagic cell death / lateral ventricle development / negative regulation of cell cycle G1/S phase transition ...cyclin D2-CDK6 complex / cyclin D2-CDK4 complex / cell dedifferentiation / Drug-mediated inhibition of CDK4/CDK6 activity / Evasion of Oncogene Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / Evasion of Oxidative Stress Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / FBXO family protein binding / autophagic cell death / lateral ventricle development / negative regulation of cell cycle G1/S phase transition / negative regulation of myeloid cell differentiation / type B pancreatic cell development / negative regulation of monocyte differentiation / astrocyte development / dentate gyrus development / negative regulation of phosphorylation / negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage / cyclin-dependent protein serine/threonine kinase inhibitor activity / response to vitamin D / gliogenesis / regulation of cell motility / Regulation of RUNX1 Expression and Activity / regulation of hematopoietic stem cell differentiation / positive regulation of cell-matrix adhesion / generation of neurons / regulation of cyclin-dependent protein serine/threonine kinase activity / DNA synthesis involved in DNA repair / regulation of G1/S transition of mitotic cell cycle / Defective binding of RB1 mutants to E2F1,(E2F2, E2F3) / negative regulation of cellular senescence / negative regulation of cell differentiation / negative regulation of cell cycle / cyclin-dependent protein kinase holoenzyme complex / hematopoietic stem cell differentiation / negative regulation of osteoblast differentiation / cyclin-dependent kinase / cyclin-dependent protein serine/threonine kinase activity / response to UV / response to retinoic acid / Notch signaling pathway / ruffle / regulation of G2/M transition of mitotic cell cycle / cyclin binding / : / sensory perception of sound / response to virus / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / regulation of erythrocyte differentiation / Oncogene Induced Senescence / negative regulation of cell growth / G1/S transition of mitotic cell cycle / Cyclin D associated events in G1 / negative regulation of epithelial cell proliferation / positive regulation of fibroblast proliferation / T cell differentiation in thymus / Senescence-Associated Secretory Phenotype (SASP) / regulation of gene expression / Oxidative Stress Induced Senescence / regulation of cell cycle / cell cycle / negative regulation of cell population proliferation / cell division / protein phosphorylation / protein serine kinase activity / centrosome / positive regulation of gene expression / protein kinase binding / negative regulation of transcription by RNA polymerase II / signal transduction / nucleoplasm / ATP binding / nucleus / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
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手法 | ![]() ![]() ![]() | ||||||
![]() | Russo, A.A. / Tong, L. / Lee, J.O. / Jeffrey, P.D. / Pavletich, N.P. | ||||||
![]() | ![]() タイトル: Structural basis for inhibition of the cyclin-dependent kinase Cdk6 by the tumour suppressor p16INK4a. 著者: Russo, A.A. / Tong, L. / Lee, J.O. / Jeffrey, P.D. / Pavletich, N.P. | ||||||
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 169.6 KB | 表示 | ![]() |
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PDB形式 | ![]() | 135.9 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 392.8 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 439.2 KB | 表示 | |
XML形式データ | ![]() | 23 KB | 表示 | |
CIF形式データ | ![]() | 33.8 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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非結晶学的対称性 (NCS) | NCS oper: (Code: given Matrix: (-0.965395, 0.252602, 0.064847), ベクター: |
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要素
#1: タンパク質 | 分子量: 36987.328 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() 発現宿主: ![]() ![]() 株 (発現宿主): HI5 参照: UniProt: Q00534, 転移酵素; リンを含む基を移すもの; キナーゼ(アルコールにつなげるもの) #2: タンパク質 | 分子量: 17723.166 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 3.16 Å3/Da / 溶媒含有率: 61.07 % | |||||||||||||||||||||||||
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結晶化 | *PLUS 温度: 4 ℃ / pH: 6.5 / 手法: 蒸気拡散法, ハンギングドロップ法 | |||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 110 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: RIGAKU / 検出器: IMAGE PLATE / 日付: 1998年1月1日 / 詳細: MIRRORS |
放射 | モノクロメーター: MIRRORS / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.006 Å / 相対比: 1 |
反射 | 解像度: 2.8→20 Å / Num. obs: 33400 / % possible obs: 96.9 % / Observed criterion σ(I): 0 / 冗長度: 2.65 % / Rsym value: 0.055 / Net I/σ(I): 14.5 |
反射 シェル | 解像度: 2.8→2.9 Å / 冗長度: 2.3 % / Mean I/σ(I) obs: 4.1 / Rsym value: 0.32 / % possible all: 91.3 |
反射 | *PLUS Num. measured all: 88741 / Rmerge(I) obs: 0.055 |
反射 シェル | *PLUS % possible obs: 91.3 % / Rmerge(I) obs: 0.32 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: BNL-22910 解像度: 2.8→10 Å / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 1 / 詳細: REFINED WITH NCS STRICT THROUGHOUT
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原子変位パラメータ |
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精密化ステップ | サイクル: LAST / 解像度: 2.8→10 Å
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拘束条件 |
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Refine LS restraints NCS | NCS model details: STRICT | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ソフトウェア | *PLUS 名称: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS |