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Open data
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Basic information
Entry | Database: PDB / ID: 1bht | ||||||
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Title | NK1 FRAGMENT OF HUMAN HEPATOCYTE GROWTH FACTOR | ||||||
![]() | HEPATOCYTE GROWTH FACTOR![]() | ||||||
![]() | HEPARIN-BINDING DOMAIN / ![]() ![]() | ||||||
Function / homology | ![]() positive regulation of neuron projection regeneration / regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling / regulation of p38MAPK cascade / Drug-mediated inhibition of MET activation / MET activates STAT3 / skeletal muscle cell proliferation / negative regulation of hydrogen peroxide-mediated programmed cell death / positive regulation of myelination / MET interacts with TNS proteins / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ultsch, M.H. / Lokker, N.A. / Godowski, P.J. / De Vos, A.M. | ||||||
![]() | ![]() Title: Crystal structure of the NK1 fragment of human hepatocyte growth factor at 2.0 A resolution. Authors: Ultsch, M. / Lokker, N.A. / Godowski, P.J. / de Vos, A.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 86.5 KB | Display | ![]() |
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PDB format | ![]() | 70 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 265 KB | Display | ![]() |
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Full document | ![]() | 274.8 KB | Display | |
Data in XML | ![]() | 10.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | ![]() Mass: 20309.295 Da / Num. of mol.: 2 Fragment: NK1 FRAGMENT, HEPARIN BINDING DOMAIN PLUS C-MET BINDING DOMAIN Source method: isolated from a genetically manipulated source Details: HEPES BUFFER MOLECULE BOUND IN KRINGLE BINDING POCKET Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() #2: Chemical | ![]() #3: Chemical | ![]() #4: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.82 Å3/Da / Density % sol: 56 % | |||||||||||||||||||||||||
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Crystal grow![]() | pH: 7.5 Details: 40% PEG 3400, 0.1M HEPES PH 7.5, 0.3M AMMONIUM SULFATE. PROTEIN CONCENTRATION OF 3MG/ML | |||||||||||||||||||||||||
Crystal | *PLUS | |||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, sitting drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 287 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: FUJI / Detector: IMAGE PLATE / Date: Jan 1, 1994 / Details: COLLIMATOR |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Highest resolution: 2 Å / Num. obs: 1807 / % possible obs: 94 % / Observed criterion σ(I): 0 / Redundancy: 5.2 % / Rmerge(I) obs: 0.066 / Rsym value: 0.056 / Net I/σ(I): 11.7 |
Reflection shell | Resolution: 2→2.09 Å / Redundancy: 3 % / Rmerge(I) obs: 0.17 / Rsym value: 0.26 / % possible all: 87 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Displacement parameters | Biso mean: 37.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→10 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: NONRESTRAINED | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2→2.09 Å / Total num. of bins used: 8
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Xplor file |
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