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Open data
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Basic information
Entry | Database: PDB / ID: 1bel | ||||||
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Title | HYDROLASE PHOSPHORIC DIESTER, RNA | ||||||
![]() | RIBONUCLEASE A | ||||||
![]() | HYDROLASE / HYDROLASE (PHOSPHORIC DIESTER / RNA) / NUCLEASE / ENDONUCLEASE / GLYCOPROTEIN | ||||||
Function / homology | ![]() pancreatic ribonuclease / ribonuclease A activity / RNA nuclease activity / nucleic acid binding / lyase activity / defense response to Gram-positive bacterium / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Dung, M.H. / Bell, J.A. | ||||||
![]() | ![]() Title: Structure of crystal form IX of bovine pancreatic ribonuclease A. Authors: Dung, M.H. / Bell, J.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 34.7 KB | Display | ![]() |
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PDB format | ![]() | 25.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 428.1 KB | Display | ![]() |
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Full document | ![]() | 428.9 KB | Display | |
Data in XML | ![]() | 8 KB | Display | |
Data in CIF | ![]() | 10.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3rn3S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 13708.326 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: PH 4.5 / Source: (natural) ![]() ![]() | ||||
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#2: Chemical | #3: Chemical | ChemComp-MOH / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.95 Å3/Da / Density % sol: 30 % | |||||||||||||||
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Crystal grow | pH: 4.5 / Details: 60% METHANOL, pH 4.50 | |||||||||||||||
Crystal | *PLUS | |||||||||||||||
Crystal grow | *PLUS Method: batch method | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 292 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Aug 28, 1992 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→10 Å / Num. obs: 12318 / % possible obs: 82 % / Observed criterion σ(I): 2 / Redundancy: 6 % / Biso Wilson estimate: 16.1 Å2 / Rmerge(I) obs: 0.083 / Net I/σ(I): 6.36 |
Reflection shell | Resolution: 1.6→1.8 Å / Mean I/σ(I) obs: 4.22 / % possible all: 61 |
Reflection | *PLUS Highest resolution: 1.6 Å / Num. measured all: 76694 |
Reflection shell | *PLUS Highest resolution: 1.6 Å / Lowest resolution: 1.8 Å |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3RN3 Resolution: 1.6→10 Å / σ(F): 0 / Stereochemistry target values: TNT PROTGEO / Details: FINAL B VALUES CALCULATED BY GRADIENT METHOD
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Solvent computation | Solvent model: BABINET / Bsol: 117 Å2 / ksol: 0.57 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.6→10 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor all: 0.15 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 1.6 Å / Lowest resolution: 1.68 Å / Rfactor obs: 0.233 |