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- PDB-1bbx: NON-SPECIFIC PROTEIN-DNA INTERACTIONS IN THE SSO7D-DNA COMPLEX, N... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1bbx | ||||||
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Title | NON-SPECIFIC PROTEIN-DNA INTERACTIONS IN THE SSO7D-DNA COMPLEX, NMR, 1 STRUCTURE | ||||||
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![]() | DNA BINDING PROTEIN/DNA / PROTEIN-DNA INTERACTION / NONSPECIFIC PROTEIN-DNA INTERACTION / COMPLEX (DNA-BINDING PROTEIN-DNA) / DNA BINDING PROTEIN-DNA COMPLEX | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Agback, P. / Baumann, H. / Knapp, S. / Ladenstein, R. / Hard, T. | ||||||
![]() | ![]() Title: Architecture of Nonspecific Protein-DNA Interactions in the Sso7D-DNA Complex Authors: Agback, P. / Baumann, H. / Knapp, S. / Ladenstein, R. / Hard, T. #1: ![]() Title: DNA-Binding Surface of the Sso7D Protein from Sulfolobus Solfataricus Authors: Baumann, H. / Knapp, S. / Karshikoff, A. / Ladenstein, R. / Hard, T. #2: ![]() Title: Solution Structure and DNA-Binding Properties of a Thermostable Protein from the Archaeon Sulfolobus Solfataricus Authors: Baumann, H. / Knapp, S. / Lundback, T. / Ladenstein, R. / Hard, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 70.4 KB | Display | ![]() |
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PDB format | ![]() | 50.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 252.2 KB | Display | ![]() |
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Full document | ![]() | 251.9 KB | Display | |
Data in XML | ![]() | 7.4 KB | Display | |
Data in CIF | ![]() | 10.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: DNA chain | Mass: 3663.392 Da / Num. of mol.: 2 / Source method: obtained synthetically #2: Protein | Mass: 7163.403 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELLED SSO7D AND NON-LABELLED DNA |
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Sample preparation
Details | Contents: H2O:D2O 9:1 |
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Sample conditions | Ionic strength: 60 mM / pH: 5.0 / Pressure: 1 atm / Temperature: 323 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITY / Manufacturer: Varian / Model: UNITY / Field strength: 500 MHz |
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Processing
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NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST RESTRAINT ENERGY / Conformers calculated total number: 50 / Conformers submitted total number: 1 |