+Open data
-Basic information
Entry | Database: PDB / ID: 1bbu | ||||||
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Title | LYSYL-TRNA SYNTHETASE (LYSS) COMPLEXED WITH LYSINE | ||||||
Components | PROTEIN (LYSYL-TRNA SYNTHETASE) | ||||||
Keywords | LIGASE / AMINOACYL-TRNA SYNTHETASE / PROTEIN BIOSYNTHESIS | ||||||
Function / homology | Function and homology information lysine-tRNA ligase / lysine-tRNA ligase activity / lysyl-tRNA aminoacylation / tRNA aminoacylation for protein translation / ligase activity / tRNA binding / magnesium ion binding / protein homodimerization activity / ATP binding / membrane / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.7 Å | ||||||
Authors | Onesti, S. / Desogus, G. / Brevet, A. / Chen, J. / Plateau, P. / Blanquet, S. / Brick, P. | ||||||
Citation | Journal: Biochemistry / Year: 2000 Title: Structural studies of lysyl-tRNA synthetase: conformational changes induced by substrate binding. Authors: Onesti, S. / Desogus, G. / Brevet, A. / Chen, J. / Plateau, P. / Blanquet, S. / Brick, P. #1: Journal: Structure / Year: 1995 Title: Crystal Structure of the Lysyl-tRNA Synthetase (Lysu) from Escherichia Coli Authors: Onesti, S. / Miller, A.D. / Brick, P. #2: Journal: J.Biol.Chem. / Year: 1995 Title: Comparison of the Enzymatic Properties of Two Escherichia Coli Lysyl-tRNA Synthetase Species Authors: Brevet, A. / Chen, J. / Leveque, F. / Blanquet, S. / Plateau, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1bbu.cif.gz | 110.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1bbu.ent.gz | 84.6 KB | Display | PDB format |
PDBx/mmJSON format | 1bbu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bb/1bbu ftp://data.pdbj.org/pub/pdb/validation_reports/bb/1bbu | HTTPS FTP |
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-Related structure data
Related structure data | 1bbwC 1lylS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 57546.066 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Cellular location: CYTOPLASMICCytoplasm / Gene: LYSS / Plasmid: PXLYSKS1 / Production host: Escherichia coli (E. coli) / Strain (production host): PAL2103UKTR / References: UniProt: P0A8N3, lysine-tRNA ligase |
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#2: Chemical | ChemComp-LYS / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.86 Å3/Da / Density % sol: 68 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: pH 7.5 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 68 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 8 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX9.6 / Wavelength: 0.87 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jun 1, 1999 / Details: MIRROR |
Radiation | Monochromator: SILICON CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.87 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→20 Å / Num. obs: 25113 / % possible obs: 99.2 % / Redundancy: 3.5 % / Rmerge(I) obs: 0.071 / Rsym value: 7.1 / Net I/σ(I): 6 |
Reflection shell | Resolution: 2.7→2.84 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.222 / Mean I/σ(I) obs: 3.2 / Rsym value: 22.2 / % possible all: 99.1 |
Reflection | *PLUS Num. measured all: 87419 |
Reflection shell | *PLUS % possible obs: 99.1 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1LYL Resolution: 2.7→20 Å / Rfactor Rfree error: 0.008 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Details: BULK SOLVENT CORRENTION APPLIED
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Displacement parameters | Biso mean: 55 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati d res low obs: 20 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.7→2.82 Å / Rfactor Rfree error: 0.04 / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.851 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.7 Å / Lowest resolution: 20 Å / σ(F): 0 / % reflection Rfree: 5 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 55 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 2.7 Å / Rfactor Rfree: 0.48 / % reflection Rfree: 5 % / Rfactor Rwork: 0.43 |