+Open data
-Basic information
Entry | Database: PDB / ID: 1baw | ||||||
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Title | PLASTOCYANIN FROM PHORMIDIUM LAMINOSUM | ||||||
Components | PLASTOCYANIN | ||||||
Keywords | ELECTRON TRANSFER / CYANOBACTERIA / BLUE COPPER PROTEIN | ||||||
Function / homology | Function and homology information plasma membrane-derived thylakoid membrane / electron transfer activity / copper ion binding Similarity search - Function | ||||||
Biological species | Phormidium laminosum (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | Bond, C.S. / Guss, J.M. / Freeman, H.C. / Wagner, M.J. / Howe, C.J. / Bendall, D.S. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1999 Title: The structure of plastocyanin from the cyanobacterium Phormidium laminosum. Authors: Bond, C.S. / Bendall, D.S. / Freeman, H.C. / Guss, J.M. / Howe, C.J. / Wagner, M.J. / Wilce, M.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1baw.cif.gz | 67.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1baw.ent.gz | 51.8 KB | Display | PDB format |
PDBx/mmJSON format | 1baw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ba/1baw ftp://data.pdbj.org/pub/pdb/validation_reports/ba/1baw | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS oper:
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-Components
#1: Protein | Mass: 11431.800 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Phormidium laminosum (bacteria) / Cell line: BL21 / Gene: PETE / Plasmid: PET11D / Gene (production host): PETE / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) PLYSS / References: UniProt: Q51883 #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 3 |
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-Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 53 % | ||||||||||||||||||||
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Crystal grow | pH: 6 / Details: pH 6.0 | ||||||||||||||||||||
Crystal grow | *PLUS Temperature: 277 K / Method: unknown | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH2R / Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS / Detector: IMAGE PLATE / Date: Jun 1, 1997 / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→30 Å / Num. obs: 9030 / % possible obs: 99.9 % / Redundancy: 9 % / Biso Wilson estimate: 21 Å2 / Rsym value: 0.114 / Net I/σ(I): 10 |
Reflection shell | Resolution: 2.8→2.9 Å / Mean I/σ(I) obs: 3 / Rsym value: 0.344 / % possible all: 100 |
Reflection | *PLUS Lowest resolution: 50 Å / Num. obs: 8981 / Rmerge(I) obs: 0.114 |
Reflection shell | *PLUS Num. unique obs: 815 / Rmerge(I) obs: 0.359 / Mean I/σ(I) obs: 4.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.8→30 Å / Data cutoff high absF: 10000000 / Data cutoff low absF: 0.001 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 27 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→30 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: CONSTRAINTS/RESTRAINTS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.8→2.93 Å / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.8 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.199 / Rfactor Rfree: 0.252 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.36 / Rfactor obs: 0.27 |