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Open data
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Basic information
Entry | Database: PDB / ID: 1b9n | ||||||
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Title | REGULATOR FROM ESCHERICHIA COLI | ||||||
![]() | PROTEIN (MODE) | ||||||
![]() | TRANSCRIPTION / DNA-BINDING / GENE REGULATION / WINGED HELIX TURN HELIX / MOLYBDATE / OB FOLD | ||||||
Function / homology | ![]() ModE complex / molybdate ion transport / negative regulation of DNA-templated transcription initiation / molybdenum ion binding / cis-regulatory region sequence-specific DNA binding / negative regulation of DNA-templated transcription / positive regulation of DNA-templated transcription / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hall, D.R. / Gourley, D.G. / Hunter, W.N. | ||||||
![]() | ![]() Title: The high-resolution crystal structure of the molybdate-dependent transcriptional regulator (ModE) from Escherichia coli: a novel combination of domain folds. Authors: Hall, D.R. / Gourley, D.G. / Leonard, G.A. / Duke, E.M. / Anderson, L.A. / Boxer, D.H. / Hunter, W.N. #1: Journal: Acta Crystallogr.,Sect.D / Year: 1999 Title: Two crystal forms of ModE, the molybdate-dependent transcriptional regulator from Escherichia coli. Authors: Hall, D.R. / Gourley, D.G. / Duke, E.M. / Leonard, G.A. / Anderson, L.A. / Pau, R.N. / Boxer, D.H. / Hunter, W.N. #2: Journal: Eur.J.Biochem. / Year: 1997 Title: Characterisation of the molybdenum-responsive ModE regulatory protein and its binding to the promoter region of the modABCD (molybdenum transport) operon of Escherichia coli. Authors: Anderson, L.A. / Palmer, T. / Price, N.C. / Bornemann, S. / Boxer, D.H. / Pau, R.N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 114.5 KB | Display | ![]() |
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PDB format | ![]() | 87.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 442 KB | Display | ![]() |
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Full document | ![]() | 459.3 KB | Display | |
Data in XML | ![]() | 25 KB | Display | |
Data in CIF | ![]() | 35.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 28590.416 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-NI / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 59 % Description: A STARTING MODEL WAS DERIVED FROM MAD PHASED MAPS OF A SELENOMETHIONYL DERIVATIVE OF THIS PROTEIN. | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.5 / Details: pH 6.5 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 277 K / pH: 6 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Mar 1, 1998 |
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.09→28.63 Å / Num. obs: 268449 / % possible obs: 99 % / Redundancy: 4 % / Biso Wilson estimate: 31.98 Å2 / Rmerge(I) obs: 0.067 / Net I/σ(I): 15.4 |
Reflection shell | Resolution: 2.09→2.15 Å / Redundancy: 4 % / Rmerge(I) obs: 0.421 / Mean I/σ(I) obs: 2 / % possible all: 99.6 |
Reflection | *PLUS Num. obs: 37763 / Num. measured all: 268449 |
Reflection shell | *PLUS % possible obs: 99.6 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 39.12 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.09→28.63 Å
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Refine LS restraints |
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