+Open data
-Basic information
Entry | Database: PDB / ID: 1b98 | ||||||
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Title | NEUROTROPHIN 4 (HOMODIMER) | ||||||
Components | PROTEIN (NEUROTROPHIN-4) | ||||||
Keywords | HORMONE/GROWTH FACTOR / TARGET-DERIVED SURVIVAL FACTOR / NEUROTROPHIN 4 / NEUROTROPHIN 5 / HORMONE-GROWTH FACTOR COMPLEX | ||||||
Function / homology | Function and homology information taste bud development / sensory organ boundary specification / ganglion mother cell fate determination / NTF4 activates NTRK2 (TRKB) signaling / Activated NTRK2 signals through PLCG1 / nerve growth factor receptor binding / mechanoreceptor differentiation / nerve growth factor signaling pathway / nerve development / innervation ...taste bud development / sensory organ boundary specification / ganglion mother cell fate determination / NTF4 activates NTRK2 (TRKB) signaling / Activated NTRK2 signals through PLCG1 / nerve growth factor receptor binding / mechanoreceptor differentiation / nerve growth factor signaling pathway / nerve development / innervation / peripheral nervous system development / Activated NTRK2 signals through PI3K / regulation of neuron differentiation / Activated NTRK2 signals through RAS / epidermis development / Activated NTRK2 signals through FRS2 and FRS3 / long-term memory / cell surface receptor protein tyrosine kinase signaling pathway / adult locomotory behavior / neuron projection morphogenesis / growth factor activity / modulation of chemical synaptic transmission / memory / synaptic vesicle / positive regulation of peptidyl-serine phosphorylation / negative regulation of neuron apoptotic process / axon / dendrite / extracellular space / extracellular region Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.75 Å | ||||||
Authors | Robinson, R.C. / Radziejewski, C. / Stuart, D.I. / Jones, E.Y. / Choe, S. | ||||||
Citation | Journal: Protein Sci. / Year: 1999 Title: The structures of the neurotrophin 4 homodimer and the brain-derived neurotrophic factor/neurotrophin 4 heterodimer reveal a common Trk-binding site. Authors: Robinson, R.C. / Radziejewski, C. / Spraggon, G. / Greenwald, J. / Kostura, M.R. / Burtnick, L.D. / Stuart, D.I. / Choe, S. / Jones, E.Y. #1: Journal: Biochemistry / Year: 1995 Title: Structure of the Brain-Derived Neurotrophic Factor/Neurotrophin 3 Heterodimer Authors: Robinson, R.C. / Radziejewski, C. / Stuart, D.I. / Jones, E.Y. #2: Journal: Biochemistry / Year: 1993 Title: Heterodimers of the Neurotrophic Factors: Formation, Isolation, and Differential Stability Authors: Radziejewski, C. / Robinson, R.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1b98.cif.gz | 55 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1b98.ent.gz | 39.4 KB | Display | PDB format |
PDBx/mmJSON format | 1b98.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1b98_validation.pdf.gz | 376 KB | Display | wwPDB validaton report |
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Full document | 1b98_full_validation.pdf.gz | 391.9 KB | Display | |
Data in XML | 1b98_validation.xml.gz | 7.9 KB | Display | |
Data in CIF | 1b98_validation.cif.gz | 11.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b9/1b98 ftp://data.pdbj.org/pub/pdb/validation_reports/b9/1b98 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 13944.647 Da / Num. of mol.: 2 / Fragment: precursor residues 81-210 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: SUPPLIED BY REGENERON PHARMACUTICALS / Production host: Escherichia coli (E. coli) / References: UniProt: P34130 #2: Chemical | ChemComp-CL / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38.3 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.5 / Details: PEG 8000, PIPES, pH 6.50 | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 15 ℃ / pH: 8 / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL1-5 / Wavelength: 0.97 |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 2.75→20 Å / Num. obs: 7858 / % possible obs: 93 % / Redundancy: 2.5 % / Rmerge(I) obs: 0.031 |
Reflection | *PLUS Highest resolution: 2.35 Å |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: NT4 Resolution: 2.75→20 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 46.3 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.75→20 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.35 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |