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Yorodumi- PDB-1ddv: CRYSTAL STRUCTURE OF THE HOMER EVH1 DOMAIN WITH BOUND MGLUR PEPTIDE -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ddv | ||||||
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Title | CRYSTAL STRUCTURE OF THE HOMER EVH1 DOMAIN WITH BOUND MGLUR PEPTIDE | ||||||
Components |
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Keywords | SIGNALING PROTEIN / PROTEIN-LIGAND COMPLEX / POLYPROLINE RECOGNITION / BETA TURN | ||||||
Function / homology | Function and homology information : / PLC activating G protein-coupled glutamate receptor activity / : / negative regulation of locomotion / G protein-coupled glutamate receptor binding / Class C/3 (Metabotropic glutamate/pheromone receptors) / structural constituent of postsynapse / : / A2A adenosine receptor binding / : ...: / PLC activating G protein-coupled glutamate receptor activity / : / negative regulation of locomotion / G protein-coupled glutamate receptor binding / Class C/3 (Metabotropic glutamate/pheromone receptors) / structural constituent of postsynapse / : / A2A adenosine receptor binding / : / regulation of translational elongation / phospholipase C-activating G protein-coupled glutamate receptor signaling pathway / G protein-coupled receptor activity involved in regulation of postsynaptic membrane potential / regulation of dendritic spine maintenance / regulation of store-operated calcium entry / trans-synaptic signaling by endocannabinoid, modulating synaptic transmission / Neurexins and neuroligins / positive regulation of long-term neuronal synaptic plasticity / : / desensitization of G protein-coupled receptor signaling pathway / regulation of calcium ion import / G protein-coupled glutamate receptor signaling pathway / protein localization to synapse / neurotransmitter receptor activity involved in regulation of postsynaptic cytosolic calcium ion concentration / astrocyte projection / neuron spine / costamere / protein kinase C-activating G protein-coupled receptor signaling pathway / positive regulation of calcium ion transport / phosphatidylinositol-mediated signaling / glutamate receptor activity / G alpha (q) signalling events / type 5 metabotropic glutamate receptor binding / postsynaptic cytosol / postsynaptic modulation of chemical synaptic transmission / plasma membrane => GO:0005886 / behavioral response to cocaine / : / skeletal muscle contraction / positive regulation of protein tyrosine kinase activity / skeletal muscle fiber development / regulation of synaptic transmission, glutamatergic / protein tyrosine kinase binding / response to cocaine / dendritic shaft / locomotory behavior / response to nicotine / learning / PDZ domain binding / G protein-coupled receptor activity / postsynaptic density membrane / protein tetramerization / synapse organization / regulation of long-term neuronal synaptic plasticity / regulation of protein phosphorylation / Schaffer collateral - CA1 synapse / cognition / Z disc / response to calcium ion / circadian rhythm / cellular response to amyloid-beta / apical part of cell / chemical synaptic transmission / postsynapse / scaffold protein binding / postsynaptic membrane / transmembrane transporter binding / dendritic spine / postsynaptic density / learning or memory / molecular adaptor activity / calmodulin binding / neuron projection / axon / protein phosphorylation / signaling receptor binding / neuronal cell body / dendrite / glutamatergic synapse / protein-containing complex binding / regulation of DNA-templated transcription / membrane / identical protein binding / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.9 Å | ||||||
Authors | Beneken, J. / Tu, J.C. / Xiao, B. / Worley, P.F. / Leahy, D.J. | ||||||
Citation | Journal: Neuron / Year: 2000 Title: Structure of the Homer EVH1 domain-peptide complex reveals a new twist in polyproline recognition. Authors: Beneken, J. / Tu, J.C. / Xiao, B. / Nuriya, M. / Yuan, J.P. / Worley, P.F. / Leahy, D.J. #1: Journal: Neuron / Year: 1998 Title: Homer binds a novel proline-rich motif and links group 1 metabotropic glutamate receptors with IP3 receptors Authors: Tu, J.C. / Xiao, B. / Yuan, J.P. / Lanahan, A.A. / Worley, P.F. / Leoffert, K. / Li, M. / Linden, D.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ddv.cif.gz | 35.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ddv.ent.gz | 24.5 KB | Display | PDB format |
PDBx/mmJSON format | 1ddv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dd/1ddv ftp://data.pdbj.org/pub/pdb/validation_reports/dd/1ddv | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 12688.221 Da / Num. of mol.: 1 / Fragment: HOMER EVH1 RESIDUES 1-111 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Organ: BRAIN / Plasmid: PGEX / Production host: Escherichia coli (E. coli) / References: UniProt: Q9Z214 |
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#2: Protein/peptide | Mass: 644.715 Da / Num. of mol.: 1 / Fragment: MGLUR DERIVED PEPTIDE TPPSPF / Source method: obtained synthetically Details: THIS PEPTIDE WAS CHEMICALLY SYNTHESIZED. THE SEQUENCE OF THIS PEPTIDE NATURALLY OCCURS IN NORWAY RAT (RATTUS NORVEGICUS) References: UniProt: P31424 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.82 Å3/Da / Density % sol: 32.46 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8 Details: PEG 3350, SODIUM ACETATE, TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 93 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Oct 8, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→30 Å / Num. all: 7996 / Num. obs: 7996 / % possible obs: 98.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 7.3 % / Rmerge(I) obs: 0.088 / Net I/σ(I): 28 |
Reflection shell | Resolution: 1.9→1.99 Å / Redundancy: 5.1 % / Rmerge(I) obs: 0.377 / % possible all: 87 |
Reflection | *PLUS |
Reflection shell | *PLUS Mean I/σ(I) obs: 5.1 |
-Processing
Software |
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Refinement | Resolution: 1.9→30 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: ENGH & HUBER
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Refinement step | Cycle: LAST / Resolution: 1.9→30 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.241 / Rfactor Rfree: 0.289 / % reflection Rfree: 10 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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