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Open data
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Basic information
Entry | Database: PDB / ID: 1ay7 | ||||||
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Title | RIBONUCLEASE SA COMPLEX WITH BARSTAR | ||||||
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![]() | COMPLEX (ENZYME/INHIBITOR) / RIBONUCLEASE / INHIBITOR / STREPTOMYCES AUREOFACIENS / COMPLEX (ENZYME-INHIBITOR) / COMPLEX (ENZYME-INHIBITOR) complex | ||||||
Function / homology | ![]() ribonuclease T1 activity / ribonuclease T1 / RNA endonuclease activity / lyase activity / RNA binding / extracellular region / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sevcik, J. / Urbanikova, L. / Dauter, Z. / Wilson, K.S. | ||||||
![]() | ![]() Title: Recognition of RNase Sa by the inhibitor barstar: structure of the complex at 1.7 A resolution. Authors: Sevcik, J. / Urbanikova, L. / Dauter, Z. / Wilson, K.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 50.4 KB | Display | ![]() |
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PDB format | ![]() | 38.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 423.8 KB | Display | ![]() |
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Full document | ![]() | 431.5 KB | Display | |
Data in XML | ![]() | 12.3 KB | Display | |
Data in CIF | ![]() | 17.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1brsS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 10582.492 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein | Mass: 10221.543 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: BARNASE INHIBITOR / Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.35 Å3/Da / Density % sol: 69 % | |||||||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7.0 | |||||||||||||||||||||||||
Crystal | *PLUS | |||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.2 / Method: vapor diffusion | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Nov 1, 1996 / Details: MIRRORS |
Radiation | Monochromator: SI(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.95 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→25 Å / Num. obs: 27413 / % possible obs: 100 % / Observed criterion σ(I): -3 / Redundancy: 4.7 % / Biso Wilson estimate: 20 Å2 / Rmerge(I) obs: 0.046 / Rsym value: 0.046 / Net I/σ(I): 27.3 |
Reflection shell | Resolution: 1.7→1.72 Å / Redundancy: 4.2 % / Rmerge(I) obs: 0.308 / Mean I/σ(I) obs: 3.7 / Rsym value: 0.308 / % possible all: 99.8 |
Reflection | *PLUS Num. all: 27413 / Num. obs: 22844 / Num. measured all: 118438 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1BRS Resolution: 1.7→25 Å / Cross valid method: FREE R / σ(F): 0 Details: ESTIMATED COORDINATE ERROR. ESD FROM CRUICKSHANK (A) : 0.078 ESD FROM SIGMAA (A) : 0.061
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Displacement parameters | Biso mean: 24.8 Å2
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Refine analyze | Luzzati sigma a obs: 0.06 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→25 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor Rfree: 0.19 / Rfactor Rwork: 0.16 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |