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Yorodumi- PDB-1awz: 3D SOLUTION STRUCTURE OF HUMAN ANGIOGENIN DETERMINED BY 1H, 15N N... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1awz | ||||||
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Title | 3D SOLUTION STRUCTURE OF HUMAN ANGIOGENIN DETERMINED BY 1H, 15N NMR SPECTROSCOPY, 30 STRUCTURES | ||||||
Components | ANGIOGENIN | ||||||
Keywords | HYDROLASE / ENDORIBONUCLEASE / ANGIOGENESIS | ||||||
Function / homology | Function and homology information activation of phospholipase A2 activity / angiogenin-PRI complex / diacylglycerol biosynthetic process / tRNA-derived small RNA (tsRNA or tRNA-related fragment, tRF) biogenesis / tRNA decay / cell communication / Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters / Adherens junctions interactions / oocyte maturation / homeostatic process ...activation of phospholipase A2 activity / angiogenin-PRI complex / diacylglycerol biosynthetic process / tRNA-derived small RNA (tsRNA or tRNA-related fragment, tRF) biogenesis / tRNA decay / cell communication / Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters / Adherens junctions interactions / oocyte maturation / homeostatic process / rRNA transcription / basement membrane / RNA nuclease activity / positive regulation of phosphorylation / ovarian follicle development / positive regulation of endothelial cell proliferation / activation of protein kinase B activity / RNA endonuclease activity / actin filament polymerization / response to hormone / positive regulation of protein secretion / negative regulation of smooth muscle cell proliferation / peptide binding / placenta development / antimicrobial humoral immune response mediated by antimicrobial peptide / cell migration / actin cytoskeleton / antibacterial humoral response / heparin binding / chromosome / actin binding / growth cone / cytoplasmic vesicle / angiogenesis / endonuclease activity / negative regulation of translation / response to hypoxia / rRNA binding / defense response to Gram-positive bacterium / copper ion binding / innate immune response / signaling receptor binding / neuronal cell body / nucleolus / protein homodimerization activity / DNA binding / extracellular space / extracellular region / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY, SIMULATED ANNEALING | ||||||
Authors | Lequin, O. / Thuring, H. / Robin, M. / Lallemand, J.-Y. | ||||||
Citation | Journal: Eur.J.Biochem. / Year: 1997 Title: Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy--characterization of histidine protonation states and pKa values. Authors: Lequin, O. / Thuring, H. / Robin, M. / Lallemand, J.Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1awz.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1awz.ent.gz | 964.1 KB | Display | PDB format |
PDBx/mmJSON format | 1awz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1awz_validation.pdf.gz | 354.3 KB | Display | wwPDB validaton report |
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Full document | 1awz_full_validation.pdf.gz | 509.2 KB | Display | |
Data in XML | 1awz_validation.xml.gz | 50.5 KB | Display | |
Data in CIF | 1awz_validation.cif.gz | 90.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/aw/1awz ftp://data.pdbj.org/pub/pdb/validation_reports/aw/1awz | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 14169.036 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: PANCREATIC RIBONUCLEASE SUPERFAMILY / Source: (gene. exp.) Homo sapiens (human) / Tissue: PLASMA / Plasmid: PXL694 / Species (production host): Escherichia coli / Production host: Escherichia coli B (bacteria) / Strain (production host): B References: UniProt: P03950, Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters |
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Has protein modification | Y |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | Ionic strength: 150 mM / pH: 5 / Pressure: 1 atm / Temperature: 313 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker AMX600 / Manufacturer: Bruker / Model: AMX600 / Field strength: 600 MHz |
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-Processing
Software |
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NMR software |
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Refinement | Method: DISTANCE GEOMETRY, SIMULATED ANNEALING / Software ordinal: 1 Details: CHARMM22 PARAMETER FILE RMS DEVIATION FROM EXPERIMENTAL DATA: NOE (A) : 0.0179 DIHEDRAL ANGLES (DEGREES) : 0.99 | ||||||||||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATION / Conformers calculated total number: 30 / Conformers submitted total number: 30 |