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Yorodumi- PDB-1ash: THE STRUCTURE OF ASCARIS HEMOGLOBIN DOMAIN I AT 2.2 ANGSTROMS RES... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ash | ||||||
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Title | THE STRUCTURE OF ASCARIS HEMOGLOBIN DOMAIN I AT 2.2 ANGSTROMS RESOLUTION: MOLECULAR FEATURES OF OXYGEN AVIDITY | ||||||
Components | HEMOGLOBIN (OXY) | ||||||
Keywords | OXYGEN STORAGE | ||||||
Function / homology | Function and homology information oxygen carrier activity / oxygen binding / heme binding / extracellular space / metal ion binding Similarity search - Function | ||||||
Biological species | Ascaris suum (pig roundworm) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.15 Å | ||||||
Authors | Yang, J. / Mathews, F.S. / Kloek, A.P. / Goldberg, D.E. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1995 Title: The structure of Ascaris hemoglobin domain I at 2.2 A resolution: molecular features of oxygen avidity. Authors: Yang, J. / Kloek, A.P. / Goldberg, D.E. / Mathews, F.S. #1: Journal: J.Biol.Chem. / Year: 1993 Title: Expression, Characterization and Crystallization of Oxygen-Avid Ascaris Hemoglobin Domains Authors: Kloek, A.P. / Yang, J. / Mathews, F.S. / Goldberg, D.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ash.cif.gz | 47.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ash.ent.gz | 32.7 KB | Display | PDB format |
PDBx/mmJSON format | 1ash.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/as/1ash ftp://data.pdbj.org/pub/pdb/validation_reports/as/1ash | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: LYS 146 - HIS 147 OMEGA = 0.02 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION |
-Components
#1: Protein | Mass: 17970.203 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Ascaris suum (pig roundworm) / Plasmid: PET-8C / References: UniProt: P28316 |
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#2: Chemical | ChemComp-HEM / |
#3: Chemical | ChemComp-OXY / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.26 Å3/Da / Density % sol: 45.6 % | ||||||||||||||||||||||||
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Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Num. obs: 9127 / % possible obs: 98.7 % / Observed criterion σ(I): 1.5 |
Reflection | *PLUS Rmerge(I) obs: 0.076 |
-Processing
Software |
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Refinement | Resolution: 2.15→10 Å / σ(F): 3
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Refine analyze | Luzzati coordinate error obs: 0.24 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.15→10 Å
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Refine LS restraints |
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Refine LS restraints | *PLUS
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