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- PDB-3aly: Crystal Structure of RNase HI from Sulfolobus tokodaii with C-ter... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3aly | ||||||
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Title | Crystal Structure of RNase HI from Sulfolobus tokodaii with C-terminal deletion | ||||||
![]() | Putative uncharacterized protein ST0753 | ||||||
![]() | HYDROLASE / thermostable RNase HI / C-terminal anchor deletion | ||||||
Function / homology | ![]() ribonuclease H / RNA-DNA hybrid ribonuclease activity / DNA binding / RNA binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Angkawidjaja, C. / Takano, K. / Kanaya, S. | ||||||
![]() | ![]() Title: Stabilization by fusion to the C-terminus of hyperthermophile Sulfolobus tokodaii RNase HI: a possibility of protein stabilization tag Authors: Takano, K. / Okamoto, T. / Okada, J. / Tanaka, S. / Angkawidjaja, C. / Koga, Y. / Kanaya, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 72.4 KB | Display | ![]() |
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PDB format | ![]() | 53.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 433.8 KB | Display | ![]() |
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Full document | ![]() | 440.7 KB | Display | |
Data in XML | ![]() | 15.6 KB | Display | |
Data in CIF | ![]() | 22.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2ehgS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16235.057 Da / Num. of mol.: 2 / Fragment: residues 1-143 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q973Z8, UniProt: F9VN79*PLUS, ribonuclease H #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 45.33 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 5.5 Details: 20% PEG 3000, 0.1M citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Dec 4, 2009 / Details: mirrors |
Radiation | Monochromator: mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.66→50 Å / Num. obs: 32375 / % possible obs: 96.2 % / Observed criterion σ(F): 5 / Observed criterion σ(I): 5 / Redundancy: 3.9 % / Rmerge(I) obs: 0.034 / Rsym value: 0.031 / Net I/σ(I): 37.21 |
Reflection shell | Resolution: 1.66→1.72 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.218 / Mean I/σ(I) obs: 6.14 / Num. unique all: 3175 / Rsym value: 0.193 / % possible all: 94.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2EHG Resolution: 1.66→20.48 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.926 / SU B: 2.076 / SU ML: 0.073 / Cross valid method: THROUGHOUT / ESU R Free: 0.122 Stereochemistry target values: MAXIMUM LIKELIHOOD WITH PHASES Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 25.003 Å2
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Refinement step | Cycle: LAST / Resolution: 1.66→20.48 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.657→1.7 Å / Total num. of bins used: 20
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