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Yorodumi- PDB-1aiw: NMR STRUCTURES OF THE CELLULOSE-BINDING DOMAIN OF THE ENDOGLUCANA... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1aiw | ||||||
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Title | NMR STRUCTURES OF THE CELLULOSE-BINDING DOMAIN OF THE ENDOGLUCANASE Z FROM ERWINIA CHRYSANTHEMI, 23 STRUCTURES | ||||||
Components | ENDOGLUCANASE Z | ||||||
Keywords | CELLULOSE DEGRADATION / ENDOGLUCANASE / CELLULOSE-BINDING DOMAIN / ERWINIA CHRYSANTHEMI | ||||||
Function / homology | Function and homology information cellulase / cellulase activity / cellulose catabolic process / carbohydrate binding / extracellular region Similarity search - Function | ||||||
Biological species | Erwinia chrysanthemi (bacteria) | ||||||
Method | SOLUTION NMR / SIMULATED ANNEALING, RESTRAINED MOLECULAR DYNAMICS | ||||||
Authors | Brun, E. / Moriaud, F. / Gans, P. / Blackledge, M.J. / Barras, F. / Marion, D. | ||||||
Citation | Journal: Biochemistry / Year: 1997 Title: Solution structure of the cellulose-binding domain of the endoglucanase Z secreted by Erwinia chrysanthemi. Authors: Brun, E. / Moriaud, F. / Gans, P. / Blackledge, M.J. / Barras, F. / Marion, D. #1: Journal: Eur.J.Biochem. / Year: 1995 Title: Overproduction, Purification and Characterization of the Cellulose-Binding Domain of the Erwinia Chrysanthemi Secreted Endoglucanase Egz Authors: Brun, E. / Gans, P. / Marion, D. / Barras, F. #2: Journal: Mol.Microbiol. / Year: 1994 Title: Periplasmic Disulphide Bond Formation is Essential for Cellulase Secretion by the Plant Pathogen Erwinia Chrysanthemi Authors: Bortoli-German, I. / Brun, E. / Py, B. / Chippaux, M. / Barras, F. #3: Journal: Protein Eng. / Year: 1991 Title: Cellulase Egz of Erwinia Chrysanthemi: Structural Organization and Importance of His98 and Glu133 Residues for Catalysis Authors: Py, B. / Bortoli-German, I. / Haiech, J. / Chippaux, M. / Barras, F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1aiw.cif.gz | 219.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1aiw.ent.gz | 179.5 KB | Display | PDB format |
PDBx/mmJSON format | 1aiw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ai/1aiw ftp://data.pdbj.org/pub/pdb/validation_reports/ai/1aiw | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6660.123 Da / Num. of mol.: 1 / Fragment: C-TERMINAL CELLULOSE-BINDING DOMAIN / Mutation: T1M, A2G Source method: isolated from a genetically manipulated source Source: (gene. exp.) Erwinia chrysanthemi (bacteria) / Genus: Dickeya / Strain: 3937 / Cell line: BL21 / Cellular location: SECRETEDSecretion / Gene: CELZ / Plasmid: PET22 / Species (production host): Escherichia coli / Cellular location (production host): EXTRACELLULAR / Gene (production host): PMIA2 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P07103, cellulase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | pH: 4.6 / Temperature: 310 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker AMX 600 / Manufacturer: Bruker / Model: AMX 600 / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: SIMULATED ANNEALING, RESTRAINED MOLECULAR DYNAMICS / Software ordinal: 1 | ||||||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATION / Conformers calculated total number: 30 / Conformers submitted total number: 23 |