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- PDB-1ab3: RIBOSOMAL PROTEIN S15 FROM THERMUS THERMOPHILUS, NMR, 26 STRUCTURES -

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Basic information

Entry
Database: PDB / ID: 1ab3
TitleRIBOSOMAL PROTEIN S15 FROM THERMUS THERMOPHILUS, NMR, 26 STRUCTURES
ComponentsRIBOSOMAL RNA BINDING PROTEIN S15
KeywordsRIBOSOMAL PROTEIN / RNA-BINDING PROTEIN / RRNA-BINDING PROTEIN
Function / homology
Function and homology information


ribosome / rRNA binding / structural constituent of ribosome / translation / ribonucleoprotein complex / cytoplasm
Similarity search - Function
S15/NS1, RNA-binding / Ribosomal protein S15, bacterial-type / Ribosomal protein S15 signature. / Ribosomal protein S15 / Ribosomal_S15 / Ribosomal protein S15 / Helix Hairpins / S15/NS1, RNA-binding / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Small ribosomal subunit protein uS15
Similarity search - Component
Biological speciesThermus thermophilus (bacteria)
MethodSOLUTION NMR / simulated annealing
AuthorsBerglund, H. / Rak, A. / Serganov, A. / Garber, M. / Hard, T.
CitationJournal: Nat.Struct.Biol. / Year: 1997
Title: Solution structure of the ribosomal RNA binding protein S15 from Thermus thermophilus.
Authors: Berglund, H. / Rak, A. / Serganov, A. / Garber, M. / Hard, T.
History
DepositionFeb 3, 1997Processing site: BNL
Revision 1.0Apr 1, 1997Provider: repository / Type: Initial release
Revision 1.1Mar 3, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 16, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: RIBOSOMAL RNA BINDING PROTEIN S15


Theoretical massNumber of molelcules
Total (without water)10,4471
Polymers10,4471
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)26 / 50LOWEST ENERGY
Representative

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Components

#1: Protein RIBOSOMAL RNA BINDING PROTEIN S15


Mass: 10447.213 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Thermus thermophilus (bacteria) / Cell line: BL21 / Plasmid: PET11C / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P80378

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experimentType: 3D HETERONUCLEAR EXPERIMENTS
NMR detailsText: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N LABELLED S15. IONIC_STRENGTH: 200 MM LICL, 20 MM KH2PO4 PRESSURE: 1 ATM SOLVENT SYSTEM: 90/10 % H2O/D2O

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Sample preparation

Sample conditionspH: 5.0 / Temperature: 303 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

NMR spectrometerType: Varian UNITY / Manufacturer: Varian / Model: UNITY / Field strength: 500 MHz

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Processing

Software
NameClassification
X-PLORmodel building
X-PLORrefinement
X-PLORphasing
NMR software
NameDeveloperClassification
X-PLORBRUNGERrefinement
X-PLORstructure solution
RefinementMethod: simulated annealing / Software ordinal: 1 / Details: DETAILS IN THE JRNL CITATION ABOVE
NMR ensembleConformer selection criteria: LOWEST ENERGY / Conformers calculated total number: 50 / Conformers submitted total number: 26

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