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Yorodumi- PDB-1ab3: RIBOSOMAL PROTEIN S15 FROM THERMUS THERMOPHILUS, NMR, 26 STRUCTURES -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ab3 | ||||||
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Title | RIBOSOMAL PROTEIN S15 FROM THERMUS THERMOPHILUS, NMR, 26 STRUCTURES | ||||||
Components | RIBOSOMAL RNA BINDING PROTEIN S15 | ||||||
Keywords | RIBOSOMAL PROTEIN / RNA-BINDING PROTEIN / RRNA-BINDING PROTEIN | ||||||
Function / homology | Function and homology information ribosome / rRNA binding / structural constituent of ribosome / translation / ribonucleoprotein complex / cytoplasm Similarity search - Function | ||||||
Biological species | Thermus thermophilus (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Berglund, H. / Rak, A. / Serganov, A. / Garber, M. / Hard, T. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1997 Title: Solution structure of the ribosomal RNA binding protein S15 from Thermus thermophilus. Authors: Berglund, H. / Rak, A. / Serganov, A. / Garber, M. / Hard, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ab3.cif.gz | 756.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ab3.ent.gz | 660.9 KB | Display | PDB format |
PDBx/mmJSON format | 1ab3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ab/1ab3 ftp://data.pdbj.org/pub/pdb/validation_reports/ab/1ab3 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10447.213 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus (bacteria) / Cell line: BL21 / Plasmid: PET11C / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P80378 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 3D HETERONUCLEAR EXPERIMENTS |
NMR details | Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N LABELLED S15. IONIC_STRENGTH: 200 MM LICL, 20 MM KH2PO4 PRESSURE: 1 ATM SOLVENT SYSTEM: 90/10 % H2O/D2O |
-Sample preparation
Sample conditions | pH: 5.0 / Temperature: 303 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITY / Manufacturer: Varian / Model: UNITY / Field strength: 500 MHz |
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-Processing
Software |
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NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 / Details: DETAILS IN THE JRNL CITATION ABOVE | |||||||||
NMR ensemble | Conformer selection criteria: LOWEST ENERGY / Conformers calculated total number: 50 / Conformers submitted total number: 26 |