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- PDB-1a93: NMR SOLUTION STRUCTURE OF THE C-MYC-MAX HETERODIMERIC LEUCINE ZIP... -

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Basic information

Entry
Database: PDB / ID: 1a93
TitleNMR SOLUTION STRUCTURE OF THE C-MYC-MAX HETERODIMERIC LEUCINE ZIPPER, NMR, MINIMIZED AVERAGE STRUCTURE
Components
  • MAX PROTEIN
  • MYC PROTO-ONCOGENE PROTEIN
KeywordsLEUCINE ZIPPER / 2D NMR / SOLUTION STRUCTURE / H-BONDS / BURIED SALT BRIDGE / PROTO-ONCOGENE / NUCLEAR PROTEIN
Function / homology
Function and homology information


Transcriptional Regulation by E2F6 / Mad-Max complex / SCF ubiquitin ligase complex binding / positive regulation of metanephric cap mesenchymal cell proliferation / negative regulation of transcription initiation by RNA polymerase II / Myc-Max complex / RNA polymerase II transcription repressor complex / regulation of cell cycle process / regulation of somatic stem cell population maintenance / Binding of TCF/LEF:CTNNB1 to target gene promoters ...Transcriptional Regulation by E2F6 / Mad-Max complex / SCF ubiquitin ligase complex binding / positive regulation of metanephric cap mesenchymal cell proliferation / negative regulation of transcription initiation by RNA polymerase II / Myc-Max complex / RNA polymerase II transcription repressor complex / regulation of cell cycle process / regulation of somatic stem cell population maintenance / Binding of TCF/LEF:CTNNB1 to target gene promoters / RUNX3 regulates WNT signaling / TFAP2 (AP-2) family regulates transcription of cell cycle factors / negative regulation of cell division / negative regulation of monocyte differentiation / DNA methylation-dependent heterochromatin formation / Transcription of E2F targets under negative control by DREAM complex / protein-DNA complex disassembly / transcription regulator activator activity / response to growth factor / negative regulation of stress-activated MAPK cascade / regulation of telomere maintenance / fibroblast apoptotic process / cellular response to peptide hormone stimulus / Regulation of NFE2L2 gene expression / positive regulation of mesenchymal cell proliferation / branching involved in ureteric bud morphogenesis / Signaling by ALK / E-box binding / MLL1 complex / positive regulation of cysteine-type endopeptidase activity involved in apoptotic process / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / chromosome organization / response to axon injury / Cyclin E associated events during G1/S transition / negative regulation of fibroblast proliferation / core promoter sequence-specific DNA binding / Cyclin A:Cdk2-associated events at S phase entry / positive regulation of telomerase activity / ERK1 and ERK2 cascade / cellular response to starvation / positive regulation of epithelial cell proliferation / transcription coregulator binding / protein-DNA complex / response to gamma radiation / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / G1/S transition of mitotic cell cycle / MAPK6/MAPK4 signaling / response to insulin / PML body / NOTCH1 Intracellular Domain Regulates Transcription / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / DNA-binding transcription repressor activity, RNA polymerase II-specific / positive regulation of miRNA transcription / Transcriptional regulation of granulopoiesis / RNA polymerase II transcription regulator complex / cellular response to UV / positive regulation of fibroblast proliferation / MAPK cascade / cellular response to xenobiotic stimulus / sequence-specific double-stranded DNA binding / retina development in camera-type eye / cellular response to hypoxia / regulation of gene expression / DNA-binding transcription activator activity, RNA polymerase II-specific / protein-containing complex assembly / neuron apoptotic process / Interleukin-4 and Interleukin-13 signaling / DNA-binding transcription factor binding / Estrogen-dependent gene expression / intracellular iron ion homeostasis / sequence-specific DNA binding / protein dimerization activity / DNA-binding transcription factor activity, RNA polymerase II-specific / Ub-specific processing proteases / chromatin remodeling / response to xenobiotic stimulus / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of gene expression / dendrite / DNA damage response / positive regulation of cell population proliferation / chromatin / protein-containing complex binding / positive regulation of gene expression / nucleolus / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / negative regulation of apoptotic process / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / nucleoplasm / identical protein binding / nucleus
Similarity search - Function
Leucine zipper, Myc / Myc leucine zipper domain / Transcription regulator Myc / Transcription regulator Myc, N-terminal / Myc amino-terminal region / Helix-loop-helix DNA-binding domain / Helix-loop-helix DNA-binding domain superfamily / helix loop helix domain / Myc-type, basic helix-loop-helix (bHLH) domain / Myc-type, basic helix-loop-helix (bHLH) domain profile.
Similarity search - Domain/homology
Myc proto-oncogene protein / Protein max
Similarity search - Component
Biological speciesHomo sapiens (human)
Mus musculus (house mouse)
MethodSOLUTION NMR / simulated annealing
AuthorsLavigne, P. / Crump, M.P. / Gagne, S.M. / Hodges, R.S. / Kay, C.M. / Sykes, B.D.
CitationJournal: J.Mol.Biol. / Year: 1998
Title: Insights into the mechanism of heterodimerization from the 1H-NMR solution structure of the c-Myc-Max heterodimeric leucine zipper.
Authors: Lavigne, P. / Crump, M.P. / Gagne, S.M. / Hodges, R.S. / Kay, C.M. / Sykes, B.D.
History
DepositionApr 15, 1998Processing site: BNL
Revision 1.0Oct 21, 1998Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 16, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_conn / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: MYC PROTO-ONCOGENE PROTEIN
B: MAX PROTEIN


Theoretical massNumber of molelcules
Total (without water)7,6202
Polymers7,6202
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / 40LEAST RESTRAINT VIOLATION ONLY
Representative

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Components

#1: Protein/peptide MYC PROTO-ONCOGENE PROTEIN / COILED COIL / LZ


Mass: 3837.432 Da / Num. of mol.: 1 / Fragment: HETERODIMERIC LEUCINE ZIPPER
Source method: isolated from a genetically manipulated source
Details: BOTH C-MYC (CHAIN A) AND MAX (CHAIN B) HAVE A NON-BIOLOGICAL CGG LINKER AT THE N-TERMINUS OF THE PROTEIN
Source: (gene. exp.) Homo sapiens (human) / References: UniProt: P01106
#2: Protein/peptide MAX PROTEIN / COILED COIL / LZ


Mass: 3782.279 Da / Num. of mol.: 1 / Fragment: HETERODIMERIC LEUCINE ZIPPER
Source method: isolated from a genetically manipulated source
Details: BOTH C-MYC (CHAIN A) AND MAX (CHAIN B) HAVE A NON-BIOLOGICAL CGG LINKER AT THE N-TERMINUS OF THE PROTEIN
Source: (gene. exp.) Mus musculus (house mouse) / References: UniProt: P28574

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
111NOESY
121TOCSY
131DQF-COSY
NMR detailsText: THE STRUCTURE WAS DETERMINED USING 1H 2D NMR SPECTROSCOPY

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Sample preparation

DetailsContents: WATER
Sample conditionsIonic strength: 10mM / pH: 4.8 / Pressure: 1 atm / Temperature: 298 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Varian VXR 500VarianVXR 5005001
Varian UNITY 600VarianUNITY 6006002

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Processing

Software
NameVersionClassification
X-PLOR3.1model building
X-PLOR3.1refinement
X-PLOR3.1phasing
NMR software
NameVersionDeveloperClassification
X-PLOR3.1BRUNGERrefinement
X-PLORstructure solution
RefinementMethod: simulated annealing / Software ordinal: 1
Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE.
NMR ensembleConformer selection criteria: LEAST RESTRAINT VIOLATION ONLY
Conformers calculated total number: 40 / Conformers submitted total number: 1

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