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Open data
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Basic information
Entry | Database: PDB / ID: 1a7b | ||||||
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Title | ENGINEERING A MISFOLDED FORM OF CD2 | ||||||
![]() | CD2 | ||||||
![]() | DOMAIN SWAPPING / CD2 / OLIGOMERIZATION / PROTEIN FOLDING / PROTEIN EVOLUTION | ||||||
Function / homology | ![]() natural killer cell mediated cytotoxicity / natural killer cell activation / heterotypic cell-cell adhesion / positive regulation of interleukin-8 production / cytoplasmic side of plasma membrane / receptor tyrosine kinase binding / cell-cell adhesion / : / positive regulation of tumor necrosis factor production / positive regulation of type II interferon production ...natural killer cell mediated cytotoxicity / natural killer cell activation / heterotypic cell-cell adhesion / positive regulation of interleukin-8 production / cytoplasmic side of plasma membrane / receptor tyrosine kinase binding / cell-cell adhesion / : / positive regulation of tumor necrosis factor production / positive regulation of type II interferon production / cell-cell junction / external side of plasma membrane / signaling receptor binding / protein kinase binding / Golgi apparatus / cell surface / protein-containing complex / extracellular region / nucleoplasm / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Murray, A.J. / Head, J.G. / Barker, J.J. / Brady, R.L. | ||||||
![]() | ![]() Title: Engineering an intertwined form of CD2 for stability and assembly. Authors: Murray, A.J. / Head, J.G. / Barker, J.J. / Brady, R.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 81.4 KB | Display | ![]() |
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PDB format | ![]() | 63.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 441.4 KB | Display | ![]() |
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Full document | ![]() | 451.9 KB | Display | |
Data in XML | ![]() | 15.6 KB | Display | |
Data in CIF | ![]() | 19 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1a64C ![]() 1a6pC ![]() 1hngS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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2 | ![]()
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3 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.7508, -0.4638, 0.4704), Vector: |
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Components
#1: Protein | Mass: 10898.259 Da / Num. of mol.: 4 / Fragment: DOMAIN 1 / Mutation: DEL(M46, K47) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 31.8 % | |||||||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: 2M AMMONIUM SULFATE 2% PEG 400, 0.1M HEPES, PH 7.5 | |||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 287 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Aug 1, 1997 / Details: MIRRORS |
Radiation | Monochromator: MIRRORS / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.488 Å / Relative weight: 1 |
Reflection | Resolution: 2→15 Å / Num. obs: 10589 / % possible obs: 99.1 % / Observed criterion σ(I): 0 / Redundancy: 2.89 % / Biso Wilson estimate: 48.86 Å2 / Rmerge(I) obs: 0.059 / Rsym value: 0.059 / Net I/σ(I): 16.1 |
Reflection shell | Resolution: 2.08→2.15 Å / Rmerge(I) obs: 0.295 / Mean I/σ(I) obs: 2.57 / Rsym value: 0.295 / % possible all: 99 |
Reflection shell | *PLUS % possible obs: 99 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1HNG Resolution: 3.1→15 Å / Rfactor Rfree error: 0.0035 / Data cutoff high absF: 3.1 / Data cutoff low absF: 10 / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 28.61 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→15 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: CONSTRAINTS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 3.1→3.24 Å / Rfactor Rfree error: 0.045 / Total num. of bins used: 8
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Xplor file |
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