#1: ジャーナル: Protein Eng. / 年: 1992 タイトル: A Functional Antibody Mutant with an Insertion in the Framework Region 3 Loop of the Vh Domain: Implications for Antibody Engineering 著者: Simon, T. / Rajewsky, K.
解像度: 1.8→1.88 Å / 冗長度: 3.8 % / Rmerge(I) obs: 0.361 / % possible all: 95.8
-
解析
ソフトウェア
名称
分類
MOSFLM
データ削減
AGROVATA/ROTAVATA
データ削減
MLPHARE
位相決定
PROLSQ
精密化
Agrovata
データスケーリング
ROTAVATA
データスケーリング
精密化
構造決定の手法: 多重同系置換 / 解像度: 1.8→6 Å / σ(F): 0 詳細: THE STRUCTURE HAS THREE MOLECULES PER EQUIVALENT POSITION ARRANGED AS A TRANSLATION ALONG THE Z-AXIS SUCH THAT OVERALL THE DIFFRACTION DATA SHOWS WEAK INTENSITIES FOR HKL, L=2N AND THIS ...詳細: THE STRUCTURE HAS THREE MOLECULES PER EQUIVALENT POSITION ARRANGED AS A TRANSLATION ALONG THE Z-AXIS SUCH THAT OVERALL THE DIFFRACTION DATA SHOWS WEAK INTENSITIES FOR HKL, L=2N AND THIS TRANSLATIONAL NON-CRYSTALLOGRAPHIC SYMMETRY IS ON CLEARLY BROKEN BY THE DIFFERENT CONFORMATION OF THE TAIL O HAPTEN LIGAND ADOPTED IN EACH OF THE THREE INDEPENDENTLY DETERMINED MOLECULES. THERE ARE ALSO TWO CONFORMATIONS FOUND FOR THE HAPTEN BOUND TO CHAINS L AND H.