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Open data
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Basic information
Entry | Database: PDB / ID: 1a0e | |||||||||
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Title | XYLOSE ISOMERASE FROM THERMOTOGA NEAPOLITANA | |||||||||
![]() | XYLOSE ISOMERASE | |||||||||
![]() | KETOLISOMERASE / XYLOSE METABOLISM / GLUCOSE-FRUCTOSE INTERCONVERSION / HYDRIDE TRANSFER / ALPHA-BETA BARREL / METALLOENZYME / THERMOPHILE | |||||||||
Function / homology | ![]() xylose isomerase / D-xylose metabolic process / xylose isomerase activity / magnesium ion binding / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Gallay, O. / Chopra, R. / Conti, E. / Brick, P. / Blow, D. | |||||||||
![]() | ![]() Title: Crystal Structures of Class II Xylose Isomerases from Two Thermophiles and a Hyperthermophile Authors: Gallay, O. / Chopra, R. / Conti, E. / Brick, P. / Jackson, R. / Hartley, B. / Vieille, C. / Zeikus, J.G. / Blow, D. #1: ![]() Title: Crystallization and Initial X-Ray Analysis of Xylose Isomerase from Thermotoga Neapolitana Authors: Chayen, N.E. / Conti, E. / Vieille, C. / Zeikus, J.G. #2: ![]() Title: Xyla Cloning and Sequencing and Biochemical Characterization of Xylose Isomerase from Thermotoga Neapolitana Authors: Vieille, C. / Hess, J.M. / Kelly, R.M. / Zeikus, J.G. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 188.9 KB | Display | ![]() |
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PDB format | ![]() | 150 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 439 KB | Display | ![]() |
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Full document | ![]() | 449.7 KB | Display | |
Data in XML | ![]() | 34.7 KB | Display | |
Data in CIF | ![]() | 49 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.99999, 0.00298, 0.0021), Vector: |
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Components
#1: Protein | Mass: 50831.598 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-CO / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 50 % |
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Crystal grow | pH: 7 Details: PROTEIN WAS CRYSTALLIZED FROM 14% JEFFAMINE ED 4000, 5 MM MGSO4, 0.5 MM COCL2, 50 MM MOPS, PH 7.0 (FOR DETAILS SEE REFERENCE 1). |
-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() |
Detector | Type: ENRAF-NONIUS FAST / Detector: DIFFRACTOMETER / Date: Jun 1, 1994 / Details: DUAL SLIT, COLLIMATOR |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→12.5 Å / Num. obs: 26137 / % possible obs: 96.3 % / Redundancy: 2.3 % / Biso Wilson estimate: 51.2 Å2 / Rmerge(I) obs: 0.076 / Net I/σ(I): 7.1 |
Reflection shell | Resolution: 2.7→2.88 Å / Redundancy: 1.9 % / Rmerge(I) obs: 0.158 / Mean I/σ(I) obs: 4.3 / % possible all: 93.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: XYLOSE ISOMERASE FROM THERMOANAEROBACTERIUM THERMOSULFURIGENES Resolution: 2.7→12.5 Å / Rfactor Rfree error: 0.006 / Cross valid method: A POSTERIORI Details: DISORDERED SIDE CHAINS WERE NOT INCLUDED IN REFINEMENT.
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Displacement parameters | Biso mean: 39 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→12.5 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.7→2.82 Å / Rfactor Rfree error: 0.026 / Total num. of bins used: 8
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Xplor file |
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