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- PDB-104d: DNA DUPLEXES FLANKED BY HYBRID DUPLEXES: THE SOLUTION STRUCTURE O... -

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Entry
Database: PDB / ID: 104d
TitleDNA DUPLEXES FLANKED BY HYBRID DUPLEXES: THE SOLUTION STRUCTURE OF CHIMERIC JUNCTIONS IN
ComponentsDNA/RNA (5'-R(*CP*GP*CP*G)-D(P*TP*AP*TP*AP*CP*GP*CP*G)-3')
KeywordsDNA-RNA HYBRID / DNA / RNA / JUNCTION / HYBRID / DUPLEX / DNA-RNA COMPLEX
MethodSOLUTION NMR
AuthorsZhu, L. / Salazar, M. / Reid, B.R.
CitationJournal: Biochemistry / Year: 1995
Title: DNA duplexes flanked by hybrid duplexes: the solution structure of chimeric junctions in [r(cgcg)d(TATACGCG)]2.
Authors: Zhu, L. / Salazar, M. / Reid, B.R.
Validation Report
SummaryFull reportAbout validation report
DateDeposition: Dec 16, 1994 / Release: Mar 31, 1995
RevisionDateData content typeGroupProviderType
1.0Mar 31, 1995Structure modelrepositoryInitial release
1.1Mar 24, 2008Structure modelVersion format compliance
1.2Jul 13, 2011Structure modelVersion format compliance

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Assembly

Deposited unit
A: DNA/RNA (5'-R(*CP*GP*CP*G)-D(P*TP*AP*TP*AP*CP*GP*CP*G)-3')
B: DNA/RNA (5'-R(*CP*GP*CP*G)-D(P*TP*AP*TP*AP*CP*GP*CP*G)-3')


Theoretical massNumber of molelcules
Total (without water)7,4552
Polyers7,4552
Non-polymers00
Water0
NMR ensembles
Datacriteria
Number of conformers (submitted / calculated)1 / -
Representative

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Components

#1: DNA/RNA hybrid DNA/RNA (5'-R(*CP*GP*CP*G)-D(P*TP*AP*TP*AP*CP*GP*CP*G)-3')


Mass: 3727.392 Da / Num. of mol.: 2 / Details: CHEMICALLY SYNTHESIZED / Keywords: DEOXYRIBONUCLEIC ACID/RIBONUCLEIC ACID

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Crystal grow
*PLUS
Method: other / Details: NMR

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Processing

SoftwareName: AMBER / Classification: refinement
NMR softwareName: DISCOVER / Developer: BIOSYM TECHNOLOGIES, INC. / Classification: refinement
RefinementSoftware ordinal: 1
Details: R VALUE 0.160 FINAL RMS COORD. SHIFT 0.65 ANGSTROMS NUMBER OF ATOMS USED IN REFINEMENT. NUMBER OF PROTEIN ATOMS 0 NUMBER OF NUCLEIC ACID ATOMS 766 NUMBER OF HETEROGEN ATOMS 0 NUMBER OF SOLVENT ATOMS 0
NMR ensembleConformers submitted total number: 1

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