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基本情報
登録情報 | データベース: EMDB / ID: EMD-9776 | |||||||||
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タイトル | Structure of C2S1M1-type PSII-FCPII supercomplex from diatom | |||||||||
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機能・相同性 | ![]() photosystem II assembly / photosystem II stabilization / thylakoid / photosystem II / extrinsic component of membrane / photosynthesis, light reaction / phosphate ion binding / chloroplast thylakoid membrane / photosynthesis / respiratory electron transport chain ...photosystem II assembly / photosystem II stabilization / thylakoid / photosystem II / extrinsic component of membrane / photosynthesis, light reaction / phosphate ion binding / chloroplast thylakoid membrane / photosynthesis / respiratory electron transport chain / electron transfer activity / protein stabilization / iron ion binding / heme binding 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.6 Å | |||||||||
![]() | Nagao R / Kato K / Shen JR / Miyazaki N / Akita F | |||||||||
![]() | ![]() タイトル: Structural basis for energy harvesting and dissipation in a diatom PSII-FCPII supercomplex. 著者: Ryo Nagao / Koji Kato / Takehiro Suzuki / Kentaro Ifuku / Ikuo Uchiyama / Yasuhiro Kashino / Naoshi Dohmae / Seiji Akimoto / Jian-Ren Shen / Naoyuki Miyazaki / Fusamichi Akita / ![]() 要旨: Light-harvesting antenna systems in photosynthetic organisms harvest solar energy and transfer it to the photosynthetic reaction centres to initiate charge-separation and electron-transfer reactions. ...Light-harvesting antenna systems in photosynthetic organisms harvest solar energy and transfer it to the photosynthetic reaction centres to initiate charge-separation and electron-transfer reactions. Diatoms are one of the important groups of oxyphototrophs and possess fucoxanthin chlorophyll a/c-binding proteins (FCPs) as light harvesters. The organization and association pattern of FCP with the photosystem II (PSII) core are unknown. Here we solved the structure of PSII-FCPII supercomplexes isolated from a diatom, Chaetoceros gracilis, by single-particle cryoelectron microscopy. The PSII-FCPII forms a homodimer. In each monomer, two FCP homotetramers and three FCP monomers are associated with one PSII core. The structure reveals a highly complicated protein-pigment network that is different from the green-type light-harvesting apparatus. Comparing these two systems allows the identification of energy transfer and quenching pathways. These findings provide structural insights into not only excitation-energy transfer mechanisms in the diatom PSII-FCPII, but also changes of light harvesters between the red- and green-lineage oxyphototrophs during evolution. | |||||||||
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構造ビューア | EMマップ: ![]() ![]() ![]() |
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マップデータ | ![]() | 30 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 43.7 KB 43.7 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 18.1 KB | 表示 | ![]() |
画像 | ![]() | 81.2 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.12 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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試料の構成要素
+全体 : C2S1M1-type PSII-FCPII supercomplex
+超分子 #1: C2S1M1-type PSII-FCPII supercomplex
+分子 #1: Photosystem II reaction center protein D1
+分子 #2: Photosystem II chlorophyll protein CP47
+分子 #3: Photosystem II chlorophyll protein CP43
+分子 #4: Photosystem II reaction center protein D2
+分子 #5: Cytochrome b559 subunit alpha
+分子 #6: Cytochrome b559 subunit beta
+分子 #7: Photosystem II reaction center protein H
+分子 #8: Photosystem II reaction center protein I
+分子 #9: Photosystem II reaction center protein J
+分子 #10: Photosystem II reaction center protein K
+分子 #11: Photosystem II reaction center protein L
+分子 #12: Photosystem II reaction center protein M
+分子 #13: Extrinsic protein in photosystem II
+分子 #14: Photosystem II reaction center protein T
+分子 #15: Extrinsic protein in photosystem II
+分子 #16: Cytochrome c-550
+分子 #17: Photosystem II reaction center protein Ycf12
+分子 #18: Photosystem II reaction center X protein
+分子 #19: Photosystem II reaction center protein Z
+分子 #20: Extrinsic protein in photosystem II
+分子 #21: Photosystem II reaction center protein W
+分子 #22: Unknown protein 0
+分子 #23: Unknown protein 1
+分子 #24: Unknown protein 2
+分子 #25: Fucoxanthin chlorophyll a/c-binding protein Lhcf1, FCP1
+分子 #26: Fucoxanthin chlorophyll a/c-binding protein monomer 1
+分子 #27: Fucoxanthin chlorophyll a/c-binding protein monomer 2
+分子 #28: Fucoxanthin chlorophyll a/c-binding protein monomer 3
+分子 #29: FE (II) ION
+分子 #30: CHLOROPHYLL A
+分子 #31: PHEOPHYTIN A
+分子 #32: BETA-CAROTENE
+分子 #33: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+分子 #34: BICARBONATE ION
+分子 #35: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+分子 #36: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+分子 #37: DODECYL-ALPHA-D-MALTOSIDE
+分子 #38: CA-MN4-O5 CLUSTER
+分子 #39: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+分子 #40: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...
+分子 #41: PROTOPORPHYRIN IX CONTAINING FE
+分子 #42: (3S,3'S,5R,5'R,6S,6'R,8'R)-3,5'-dihydroxy-8-oxo-6',7'-didehydro-5...
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
濃度 | 0.256 mg/mL | ||||||
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緩衝液 | pH: 6.5 / 構成要素:
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グリッド | モデル: Quantifoil R1.2/1.3 / 材質: COPPER / メッシュ: 200 / 支持フィルム - 材質: CARBON / 前処理 - タイプ: PLASMA CLEANING | ||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 平均電子線量: 20.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |