+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-9669 | |||||||||
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Title | Cryo-EM Structure of Human SRCAP full complex | |||||||||
Map data | dull complex. 8.5 Angstrom. | |||||||||
Sample |
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Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 8.5 Å | |||||||||
Authors | Feng Y / Tian Y / Wu Z / Xu Y | |||||||||
Citation | Journal: Cell Res / Year: 2018 Title: Cryo-EM structure of human SRCAP complex. Authors: Yangyang Feng / Yuan Tian / Zihan Wu / Yanhui Xu / | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_9669.map.gz | 6.9 MB | EMDB map data format | |
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Header (meta data) | emd-9669-v30.xml emd-9669.xml | 7.8 KB 7.8 KB | Display Display | EMDB header |
Images | emd_9669.png | 50.7 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-9669 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-9669 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_9669.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | dull complex. 8.5 Angstrom. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.35 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : SRCAP complex
Entire | Name: SRCAP complex |
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Components |
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-Supramolecule #1: SRCAP complex
Supramolecule | Name: SRCAP complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#5 |
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Source (natural) | Organism: Homo sapiens (human) |
Recombinant expression | Organism: Mammalia (mammals) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 1.5625 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
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Final angle assignment | Type: ANGULAR RECONSTITUTION |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 8.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 15248 |