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- EMDB-9306: Human TFIID bound to promoter DNA and TFIIA -

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Basic information

Entry
Database: EMDB / ID: EMD-9306
TitleHuman TFIID bound to promoter DNA and TFIIA
Map dataPrimary map
Sample
  • Complex: General transcription factor IID
    • Protein or peptide: x 15 types
    • DNA: x 2 types
KeywordsTranscription / DNA / Nuclear / transcription-dna complex
Function / homology
Function and homology information


negative regulation of MHC class I biosynthetic process / spermine transport / SAGA complex assembly / lateral mesodermal cell differentiation / DNA-templated transcription open complex formation / allantois development / pre-snoRNP complex / positive regulation of androgen receptor signaling pathway / TFIIH-class transcription factor complex binding / negative regulation of protein autoubiquitination ...negative regulation of MHC class I biosynthetic process / spermine transport / SAGA complex assembly / lateral mesodermal cell differentiation / DNA-templated transcription open complex formation / allantois development / pre-snoRNP complex / positive regulation of androgen receptor signaling pathway / TFIIH-class transcription factor complex binding / negative regulation of protein autoubiquitination / negative regulation of MHC class II biosynthetic process / transcription factor TFTC complex / RNA polymerase I general transcription initiation factor activity / regulation of cell cycle G1/S phase transition / RNA polymerase transcription factor SL1 complex / SLIK (SAGA-like) complex / histone H4K16ac reader activity / RNA polymerase III general transcription initiation factor activity / RNA polymerase I core promoter sequence-specific DNA binding / hepatocyte differentiation / positive regulation of response to cytokine stimulus / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / transcription factor TFIIA complex / maintenance of protein location in nucleus / C2H2 zinc finger domain binding / female germ cell nucleus / RNA Polymerase III Abortive And Retractive Initiation / male pronucleus / female pronucleus / RNA polymerase II general transcription initiation factor binding / nuclear vitamin D receptor binding / RNA polymerase binding / nuclear thyroid hormone receptor binding / regulation of fat cell differentiation / limb development / box C/D snoRNP assembly / SAGA complex / transcription preinitiation complex / RNA Polymerase I Transcription Termination / inner cell mass cell proliferation / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / histone acetyltransferase binding / midbrain development / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / cellular response to ATP / negative regulation of signal transduction by p53 class mediator / regulation of RNA splicing / transcription initiation at RNA polymerase I promoter / negative regulation of cell cycle / aryl hydrocarbon receptor binding / ubiquitin conjugating enzyme activity / TFIIB-class transcription factor binding / P-TEFb complex binding / RNA Polymerase I Transcription Initiation / MLL1 complex / transcription by RNA polymerase III / RNA polymerase II transcribes snRNA genes / negative regulation of ubiquitin-dependent protein catabolic process / positive regulation of transcription initiation by RNA polymerase II / embryonic placenta development / somitogenesis / histone acetyltransferase activity / RNA polymerase II core promoter sequence-specific DNA binding / core promoter sequence-specific DNA binding / regulation of DNA repair / negative regulation of protein kinase activity / RNA polymerase II preinitiation complex assembly / transcription regulator inhibitor activity / histone acetyltransferase / ovarian follicle development / positive regulation of intrinsic apoptotic signaling pathway / estrogen receptor signaling pathway / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / RNA Polymerase II Pre-transcription Events / response to interleukin-1 / TBP-class protein binding / regulation of signal transduction by p53 class mediator / nuclear estrogen receptor binding / nuclear receptor binding / SIRT1 negatively regulates rRNA expression / male germ cell nucleus / transcription initiation at RNA polymerase II promoter / promoter-specific chromatin binding / RNA Polymerase I Promoter Escape / DNA-templated transcription initiation / mRNA transcription by RNA polymerase II / G1/S transition of mitotic cell cycle / euchromatin / NoRC negatively regulates rRNA expression / B-WICH complex positively regulates rRNA expression
Similarity search - Function
TAFII55 protein, conserved region / Transcription initiation factor TFIID subunit 7 / TAFII55 protein conserved region / TAF2 Ig-like domain / TAFII55 protein conserved region / Transcription initiation factor TFIID subunit 2 / Transcription initiation factor TFIID subunit 8 / Transcription factor TFIID complex subunit 8 C-term / Transcription factor TFIID, subunit 8, C-terminal / Transcription initiation factor TFIID component TAF4 ...TAFII55 protein, conserved region / Transcription initiation factor TFIID subunit 7 / TAFII55 protein conserved region / TAF2 Ig-like domain / TAFII55 protein conserved region / Transcription initiation factor TFIID subunit 2 / Transcription initiation factor TFIID subunit 8 / Transcription factor TFIID complex subunit 8 C-term / Transcription factor TFIID, subunit 8, C-terminal / Transcription initiation factor TFIID component TAF4 / Transcription initiation factor TFIID component TAF4 family / Transcription initiation factor TFIID component TAF4, C-terminal / TAFH/NHR1 domain superfamily / NHR1 homology to TAF / TAFH/NHR1 domain profile. / TAF homology / TAFH/NHR1 / : / Transcription initiation factor IID, 31kD subunit / Bromodomain associated / Bromodomain transcription factors and PHD domain containing proteins / Bromodomain associated domain / Transcription initiation factor TFIID subunit 12 / Transcription initiation factor TFIID subunit A / TFIID subunit TAF5, NTD2 domain superfamily / WD40 associated region in TFIID subunit, NTD2 domain / Transcription initiation factor TAFII31 / TATA box binding protein associated factor (TAF) / Transcription initiation factor TFIID subunit 12 domain / TFIID subunit TAF5, NTD2 domain / Transcription initiation factor TFIID subunit 6 / TAF6, C-terminal HEAT repeat domain superfamily / TAF6 C-terminal HEAT repeat domain / TAF6, C-terminal HEAT repeat domain / Transcription initiation factor TFIID 23-30kDa subunit / Transcription initiation factor TFIID, 23-30kDa subunit / TAFII-230 TBP-binding / Transcription initiation factor TFIID subunit 1, animal / TAFII-230 TBP-binding domain superfamily / TATA box-binding protein binding / Zinc knuckle / Zinc knuckle / Transcription initiation factor TFIID subunit 1, histone acetyltransferase domain / Transcription initiation factor TFIID subunit 1 / Protein of unknown function (DUF3591) / Transcription factor IIA, alpha/beta subunit / Transcription factor IIA, alpha/beta subunit / Transcription factor IIA, alpha/beta subunit / Transcription initiation factor IIA, gamma subunit / Transcription factor IIA, alpha-helical domain / Transcription factor IIA, beta-barrel / Transcription initiation factor IIA, gamma subunit, C-terminal / Transcription initiation factor IIA, gamma subunit, N-terminal / Transcription initiation factor IIA, gamma subunit, helical domain / Transcription initiation factor IIA, gamma subunit / LIS1 homology (LisH) motif profile. / LIS1 homology motif / TATA-box binding protein, eukaryotic / TATA-box binding protein / TATA-box binding protein, conserved site / Transcription factor TFIID (or TATA-binding protein, TBP) / Transcription factor TFIID repeat signature. / Aminopeptidase N-like , N-terminal domain superfamliy / TBP domain superfamily / Peptidase M4/M1, CTD superfamily / TATA box binding protein associated factor / TATA box binding protein associated factor (TAF), histone-like fold domain / Bromodomain, conserved site / Bromodomain signature. / Bromodomain / Histone-fold / bromo domain / Bromodomain / Bromodomain (BrD) profile. / Bromodomain-like superfamily / WD domain, G-beta repeat / Armadillo-type fold / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Transcription initiation factor TFIID subunit 4 / TATA-box-binding protein / Transcription initiation factor TFIID subunit 1 / Transcription initiation factor TFIID subunit 6 / Transcription initiation factor IIA subunit 1 / Transcription initiation factor IIA subunit 2 / Transcription initiation factor TFIID subunit 10 / Transcription initiation factor TFIID subunit 5 / Transcription initiation factor TFIID subunit 7 / Transcription initiation factor TFIID subunit 12 ...Transcription initiation factor TFIID subunit 4 / TATA-box-binding protein / Transcription initiation factor TFIID subunit 1 / Transcription initiation factor TFIID subunit 6 / Transcription initiation factor IIA subunit 1 / Transcription initiation factor IIA subunit 2 / Transcription initiation factor TFIID subunit 10 / Transcription initiation factor TFIID subunit 5 / Transcription initiation factor TFIID subunit 7 / Transcription initiation factor TFIID subunit 12 / Transcription initiation factor TFIID subunit 9 / Transcription initiation factor TFIID subunit 2 / Transcription initiation factor TFIID subunit 8
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 7.5 Å
AuthorsPatel AB / Louder RK
Funding support United States, 2 items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI) United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-GM63072 United States
CitationJournal: Science / Year: 2018
Title: Structure of human TFIID and mechanism of TBP loading onto promoter DNA.
Authors: Avinash B Patel / Robert K Louder / Basil J Greber / Sebastian Grünberg / Jie Luo / Jie Fang / Yutong Liu / Jeff Ranish / Steve Hahn / Eva Nogales /
Abstract: The general transcription factor IID (TFIID) is a critical component of the eukaryotic transcription preinitiation complex (PIC) and is responsible for recognizing the core promoter DNA and ...The general transcription factor IID (TFIID) is a critical component of the eukaryotic transcription preinitiation complex (PIC) and is responsible for recognizing the core promoter DNA and initiating PIC assembly. We used cryo-electron microscopy, chemical cross-linking mass spectrometry, and biochemical reconstitution to determine the complete molecular architecture of TFIID and define the conformational landscape of TFIID in the process of TATA box-binding protein (TBP) loading onto promoter DNA. Our structural analysis revealed five structural states of TFIID in the presence of TFIIA and promoter DNA, showing that the initial binding of TFIID to the downstream promoter positions the upstream DNA and facilitates scanning of TBP for a TATA box and the subsequent engagement of the promoter. Our findings provide a mechanistic model for the specific loading of TBP by TFIID onto the promoter.
History
DepositionNov 5, 2018-
Header (metadata) releaseNov 28, 2018-
Map releaseNov 28, 2018-
UpdateMay 21, 2025-
Current statusMay 21, 2025Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6mzm
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_9306.png

Downloads & links

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Map

FileDownload / File: emd_9306.map.gz / Format: CCP4 / Size: 91.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationPrimary map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.32 Å/pix.
x 288 pix.
= 380.16 Å		1.32 Å/pix.
x 288 pix.
= 380.16 Å		1.32 Å/pix.
x 288 pix.
= 380.16 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.32 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.03 / Movie #1: 0.03
Minimum - Maximum-0.039078873 - 0.118663885
Average (Standard dev.)0.00023230643 (±0.0047335494)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions288288288
Spacing288288288
CellA=B=C: 380.16 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.321.321.32
M x/y/z288288288
origin x/y/z0.0000.0000.000
length x/y/z380.160380.160380.160
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS288288288
D min/max/mean-0.0390.1190.000

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Supplemental data

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Half map: em-half-volume P1

Fileemd_9306_half_map_1.map
Annotationem-half-volume_P1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: em-half-volume P2

Fileemd_9306_half_map_2.map
Annotationem-half-volume_P2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : General transcription factor IID

EntireName: General transcription factor IID
Components
  • Complex: General transcription factor IID
    • Protein or peptide: Transcription initiation factor TFIID subunit 1, TAF1
    • Protein or peptide: Transcription initiation factor TFIID subunit 2, TAF2
    • Protein or peptide: Transcription initiation factor TFIID subunit 4, TAF4
    • Protein or peptide: Transcription initiation factor TFIID subunit 5,TAF5
    • Protein or peptide: Transcription initiation factor TFIID subunit 6
    • Protein or peptide: Transcription initiation factor TFIID subunit 6
    • Protein or peptide: Transcription initiation factor TFIID subunit 7
    • Protein or peptide: Transcription initiation factor TFIID subunit 8, TAF8
    • Protein or peptide: Transcription initiation factor TFIID subunit 9, TAF9
    • Protein or peptide: Transcription initiation factor TFIID subunit 10
    • Protein or peptide: Transcription initiation factor TFIID subunit 12
    • Protein or peptide: TATA-box-binding protein
    • DNA: SCP DNA (80-MER)
    • DNA: SCP DNA (80-MER)
    • Protein or peptide: Transcription initiation factor IIA subunit 1
    • Protein or peptide: Transcription initiation factor IIA subunit 2
    • Protein or peptide: Unk

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Supramolecule #1: General transcription factor IID

SupramoleculeName: General transcription factor IID / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#17
Source (natural)Organism: Homo sapiens (human) / Strain: HeLa

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Macromolecule #1: Transcription initiation factor TFIID subunit 1, TAF1

MacromoleculeName: Transcription initiation factor TFIID subunit 1, TAF1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 57.193742 KDa
SequenceString: IIQHSIPAVE LRQPFFPTHM GPIKLRQFHR PPLKKYSFGA LSQPGPHSVQ PLLKHIKKKA KMREQERQAS GGGEMFFMRT PQDLTGKDG DLILAEYSEE NGPLMMQVGM ATKIKNYYKR KPGKDPGAPD CKYGETVYCH TSPFLGSLHP GQLLQAFENN L FRAPIYLH ...String:
IIQHSIPAVE LRQPFFPTHM GPIKLRQFHR PPLKKYSFGA LSQPGPHSVQ PLLKHIKKKA KMREQERQAS GGGEMFFMRT PQDLTGKDG DLILAEYSEE NGPLMMQVGM ATKIKNYYKR KPGKDPGAPD CKYGETVYCH TSPFLGSLHP GQLLQAFENN L FRAPIYLH KMPETDFLII RTRQGYYIRE LVDIFVVGQQ CPLFEVPGPN SKRANTHIRD FLQVFIYRLF WKSKDRPRRI RM EDIKKAF PSHSESSIRK RLKLCADFKR TGMDSNWWVL KSDFRLPTEE EIRAMVSPEQ CCAYYSMIAA EQRLKDAGYG EKS FFAPEE ENEEDFQMKI DDEVRTAPWN TTRAFIAAMK GKCLLEVTGV ADPTGCGEGF SYVKIPNKPT QQKDDKEPQP VKKT VTGTD ADLRRLSLKN AKQLLRKFGV PEEEIKKLSR WEVIDVVRTM STEQARSGEG PMSKFARGSR FSVAEHQERY KEECQ RIFD LQNKVLSSTE VL

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Macromolecule #2: Transcription initiation factor TFIID subunit 2, TAF2

MacromoleculeName: Transcription initiation factor TFIID subunit 2, TAF2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 137.159984 KDa
SequenceString: MPLTGVEPAR MNRKKGDKGF ESPRPYKLTH QVVCINNINF QRKSVVGFVE LTIFPTVANL NRIKLNSKQC RIYRVRINDL EAAFIYNDP TLEVCHSESK QRNLNYFSNA YAAAVSAVDP DAGNGELCIK VPSELWKHVD ELKVLKIHIN FSLDQPKGGL H FVVPSVEG ...String:
MPLTGVEPAR MNRKKGDKGF ESPRPYKLTH QVVCINNINF QRKSVVGFVE LTIFPTVANL NRIKLNSKQC RIYRVRINDL EAAFIYNDP TLEVCHSESK QRNLNYFSNA YAAAVSAVDP DAGNGELCIK VPSELWKHVD ELKVLKIHIN FSLDQPKGGL H FVVPSVEG SMAERGAHVF SCGYQNSTRF WFPCVDSYSE LCTWKLEFTV DAAMVAVSNG DLVETVYTHD MRKKTFHYML TI PTAASNI SLAIGPFEIL VDPYMHEVTH FCLPQLLPLL KHTTSYLHEV FEFYEEILTC RYPYSCFKTV FIDEAYVEVA AYA SMSIFS TNLLHSAMII DETPLTRRCL AQSLAQQFFG CFISRMSWSD EWVLKGISGY IYGLWMKKTF GVNEYRHWIK EELD KIVAY ELKTGGVLLH PIFGGGKEKD NPASHLHFSI KHPHTLSWEY YSMFQCKAHL VMRLIENRIS MEFMLQVFNK LLSLA STAS SQKFQSHMWS QMLVSTSGFL KSISNVSGKD IQPLIKQWVD QSGVVKFYGS FAFNRKRNVL ELEIKQDYTS PGTQKY VGP LKVTVQELDG SFNHTLQIEE NSLKHDIPCH SKSRRNKKKK IPLMNGEEVD MDLSAMDADS PLLWIRIDPD MSVLRKV EF EQADFMWQYQ LRYERDVVAQ QESILALEKF PTPASRLALT DILEQEQCFY RVRMSACFCL AKIANSMVST WTGPPAMK S LFTRMFCCKS CPNIVKTNNF MSFQSYFLQK TMPVAMALLR DVHNLCPKEV LTFILDLIKY NDNRKNKFSD NYYRAEMID ALANSVTPAV SVNNEVRTLD NLNPDVRLIL EEITRFLNME KLLPSYRHTI TVSCLRAIRV LQKNGHVPSD PALFKSYAEY GHFVDIRIA ALEAVVDYTK VDRSYEELQW LLNMIQNDPV PYVRHKILNM LTKNPPFTKN MESPLCNEAL VDQLWKLMNS G TSHDWRLR CGAVDLYFTL FGLSRPSCLP LPELGLVLNL KEKKAVLNPT IIPESVAGNQ EAANNPSSHP QLVGFQNPFS SS QDEEEID MDTVHDSQAF ISHHLNMLER PSTPGLSKYR PASSRSALIP QHSAGCDSTP TTKPQWSLEL ARKGTGKEQA PLE MSMHPA ASAPLSVFTK ESTASKHSDH HHHHHHEHKK KKKKHKHKHK HKHKHDSKEK DKEPFTFSSP ASGRSIRSPS LSD

UniProtKB: Transcription initiation factor TFIID subunit 2

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Macromolecule #3: Transcription initiation factor TFIID subunit 4, TAF4

MacromoleculeName: Transcription initiation factor TFIID subunit 4, TAF4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 104.052086 KDa
SequenceString: HVVSGSPAGA AGAGPAAPAE GAPGAAPEPP PAGRARPGGG GPQRPGPPSP RRPLVPAGPA PPAAKLRPPP EGSAGSCAPV PAAAAVAAG PEPAPAGPAK PAGPAALAAR AGPGPGPGPG PGPGPGPGKP AGPGAAQTLN GSAALLNSHH AAAPAVSLVN N GPAALLPL ...String:
HVVSGSPAGA AGAGPAAPAE GAPGAAPEPP PAGRARPGGG GPQRPGPPSP RRPLVPAGPA PPAAKLRPPP EGSAGSCAPV PAAAAVAAG PEPAPAGPAK PAGPAALAAR AGPGPGPGPG PGPGPGPGKP AGPGAAQTLN GSAALLNSHH AAAPAVSLVN N GPAALLPL PKPAAPGTVI QTPPFVGAAA PPAPAAPSPP AAPAPAAPAA APPPPPPAPA TLARPPGHPA GPPTAAPAVP PP AAAQNGG SAGAAPAPAP AAGGPAGVSG QPGPGAAAAA PAPGVKAESP KRVVQAAPPA AQTLAASGPA STAASMVIGP TMQ GALPSP AAVPPPAPGT PTGLPKGAAG AVTQSLSRTP TATTSGIRAT LTPTVLAPRL PQPPQNPTNI QNFQLPPGMV LVRS ENGQL LMIPQQALAQ MQAQAHAQPQ TTMAPRPATP TSAPPVQIST VQAPGTPIIA RQVTPTTIIK QVSQAQTTVQ PSATL QRSP GVQPQLVLGG AAQTASLGTA TAVQTGTPQR TVPGATTTSS AATETMENVK KCKNFLSTLI KLASSGKQST ETAANV KEL VQNLLDGKIE AEDFTSRLYR ELNSSPQPYL VPFLKRSLPA LRQLTPDSAA FIQQSQQQPP PPTSQATTAL TAVVLSS SV QRTAGKTAAT VTSALQPPVL SLTQPTQVGV GKQGQPTPLV IQQPPKPGAL IRPPQVTLTQ TPMVALRQPH NRIMLTTP Q QIQLNPLQPV PVVKPAVLPG TKALSAVSAQ AAAAQKNKLK EPGGGSFRDD DDINDVASMA GVNLSEESAR ILATNSELV GTLTRSCKDE TFLLQAPLQR RILEIGKKHG ITELHPDVVS YVSHATQQRL QNLVEKISET AQQKNFSYKD DDRYEQASDV RAQLKFFEQ LDQIEKQRKD EQEREILMRA AKSRSRQEDP EQLRLKQKAK EMQQQELAQM RQRDANLTAL AAIGPRKKRK V DCPGPGSG AEGSGPGSVV PGSSGVGTPR QFTRQRITRV NLRDLIFCLE NERETSHSLL LYKAFLK

UniProtKB: Transcription initiation factor TFIID subunit 4

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Macromolecule #4: Transcription initiation factor TFIID subunit 5,TAF5

MacromoleculeName: Transcription initiation factor TFIID subunit 5,TAF5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 85.785164 KDa
SequenceString: MAALAEEQTE VAVKLEPEGP PTLLPPQAGD GAGEGSGGTT NNGPNGGGGN VAASSSTGGD GGTPKPTVAV SAAAPAGAAP VPAAAPDAG APHDRQTLLA VLQFLRQSKL REAEEALRRE AGLLEEAVAG SGAPGEVDSA GAEVTSALLS RVTASAPGPA A PDPPGTGA ...String:
MAALAEEQTE VAVKLEPEGP PTLLPPQAGD GAGEGSGGTT NNGPNGGGGN VAASSSTGGD GGTPKPTVAV SAAAPAGAAP VPAAAPDAG APHDRQTLLA VLQFLRQSKL REAEEALRRE AGLLEEAVAG SGAPGEVDSA GAEVTSALLS RVTASAPGPA A PDPPGTGA SGATVVSGSA SGPAAPGKVG SVAVEDQPDV SAVLSAYNQQ GDPTMYEEYY SGLKHFIECS LDCHRAELSQ LF YPLFVHM YLELVYNQHE NEAKSFFEKF HGDQECYYQD DLRVLSSLTK KEHMKGNETM LDFRTSKFVL RISRDSYQLL KRH LQEKQN NQIWNIVQEH LYIDIFDGMP RSKQQIDAMV GSLAGEAKRE ANKSKVFFGL LKEPEIEVPL DDEDEEGENE EGKP KKKKP KKDSIGSKSK KQDPNAPPQN RIPLPELKDS DKLDKIMNMK ETTKRVRLGP DCLPSICFYT FLNAYQGLTA VDVTD DSSL IAGGFADSTV RV(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)KILYGH SGPVYGASFS PDRN YLLSS SEDGTVRLWS LQTFTCLVGY KGHNYPVWDT QFSPYGYYFV SGGHDRVARL WATDHYQPLR IFAGHLADVN CTRFH PNSN YVATGSADRT VRLWDVLNGN CVRIFTGHKG PIHSLTFSPN GRFLATGATD GRVLLWDIGH GLMVGELKGH TDTVCS LRF SRDGEILASG SMDNTVRLWD AIKAFEDLET DDFTTATGHI NLPENSQELL LGTYMTKSTP VVHLHFTRRN LVLAAGA YS PQ

UniProtKB: Transcription initiation factor TFIID subunit 5, Transcription initiation factor TFIID subunit 5

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Macromolecule #5: Transcription initiation factor TFIID subunit 6

MacromoleculeName: Transcription initiation factor TFIID subunit 6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 72.749297 KDa
SequenceString: MAEEKKLKLS NTVLPSESMK VVAESMGIAQ IQEETCQLLT DEVSYRIKEI AQDALKFMHM GKRQKLTTSD IDYALKLKNV EPLYGFHAQ EFIPFRFASG GGRELYFYEE KEVDLSDIIN TPLPRVPLDV CLKAHWLSIE GCQPAIPENP PPAPKEQQKA E ATEPLKSA ...String:
MAEEKKLKLS NTVLPSESMK VVAESMGIAQ IQEETCQLLT DEVSYRIKEI AQDALKFMHM GKRQKLTTSD IDYALKLKNV EPLYGFHAQ EFIPFRFASG GGRELYFYEE KEVDLSDIIN TPLPRVPLDV CLKAHWLSIE GCQPAIPENP PPAPKEQQKA E ATEPLKSA KPGQEEDGPL KGKGQGATTA DGKGKEKKAP PLLEGAPLRL KPRSIHELSV EQQLYYKEIT EACVGSCEAK RA EALQSIA TDPGLYQMLP RFSTFISEGV RVNVVQNNLA LLIYLMRMVK ALMDNPTLYL EKYVHELIPA VMTCIVSRQL CLR PDVDNH WALRDFAARL VAQICKHFST TTNNIQSRIT KTFTKSWVDE KTPWTTRYGS IAGLAELGHD VIKTLILPRL QQEG ERIRS VLDGPVLSNI DRIGADHVQS LLLKHCAPVL AKLRPPPDNQ DAYRAEFGSL GPLLCSQVVK ARAQAALQAQ QVNRT TLTI TQPRPTLTLS QAPQPGPRTP GLLKVPGSIA LPVQTLVSAR AAAPPQPSPP PTKFIVMSSS SSAPSTQQVL SLSTSA PGS GSTTTSPVTT TVPSVQPIVK LVSTATTAPP STAPSGPGSV QKYIVVSLPP TGEGKGGPTS HPSPVPPPAS SPSPLSG SA LCGGKQEAGD SPPPAPGTPK ANGSQPNSGS PQPAP

UniProtKB: Transcription initiation factor TFIID subunit 6

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Macromolecule #6: Transcription initiation factor TFIID subunit 6

MacromoleculeName: Transcription initiation factor TFIID subunit 6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 72.365836 KDa
SequenceString: MAEEKKLKLS NTVLPSESMK VVAESMGIAQ IQEETCQLLT DEVSYRIKEI AQDALKFMHM GKRQKLTTSD IDYALKLKNV EPLYGFHAQ EFIPFRFASG GGRELYFYEE KEVDLSDIIN TPLPRVPLDV CLKAHWLSIE GCQPAIPENP PPAPKEQQKA E ATEPLKSA ...String:
MAEEKKLKLS NTVLPSESMK VVAESMGIAQ IQEETCQLLT DEVSYRIKEI AQDALKFMHM GKRQKLTTSD IDYALKLKNV EPLYGFHAQ EFIPFRFASG GGRELYFYEE KEVDLSDIIN TPLPRVPLDV CLKAHWLSIE GCQPAIPENP PPAPKEQQKA E ATEPLKSA KPGQEEDGPL KGKGQGATTA DGKGKEKKAP PLLEGAPL(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)S VEQQLYYKEI TEACVGSCEA KRAEALQSIA TDPGLYQMLP RFSTFISEGV RVNVVQNNL ALLIYLMRMV KALMDNPTLY LEKYVHELIP AVMTCIVSRQ LCLRPDVDNH WALRDFAARL VAQICKHFST TTNNIQSRIT KTFTKSWVD EKTPWTTRYG SIAGLAELGH DVIKTLILPR LQQEGERIRS VLDGPVLSNI DRIGADHVQS LLLKHCAPVL A KLRPPPDN QDAYRAEFGS LGPLLCSQVV KARAQAALQA QQVNRTTLTI TQPRPTLTLS QAPQPGPRTP GLLKVPGSIA LP VQTLVSA RAAAPPQPSP PPTKFIVMSS SSSAPSTQQV LSLSTSAPGS GSTTTSPVTT TVPSVQPIVK LVSTATTAPP STA PSGPGS VQKYIVVSLP PTGEGKGGPT SHPSPVPPPA SSPSPLSGSA LCGGKQEAGD SPPPAPGTPK ANGSQPNSGS PQPA P

UniProtKB: Transcription initiation factor TFIID subunit 6, Transcription initiation factor TFIID subunit 6

+
Macromolecule #7: Transcription initiation factor TFIID subunit 7

MacromoleculeName: Transcription initiation factor TFIID subunit 7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 40.325117 KDa
SequenceString: MSKSKDDAPH ELESQFILRL PPEYASTVRR AVQSGHVNLK DRLTIELHPD GRHGIVRVDR VPLASKLVDL PCVMESLKTI DKKTFYKTA DICQMLVSTV DGDLYPPVEE PVASTDPKAS KKKDKDKEKK FIWNHGITLP LKNVRKRRFR KTAKKKYIES P DVEKEVKR ...String:
MSKSKDDAPH ELESQFILRL PPEYASTVRR AVQSGHVNLK DRLTIELHPD GRHGIVRVDR VPLASKLVDL PCVMESLKTI DKKTFYKTA DICQMLVSTV DGDLYPPVEE PVASTDPKAS KKKDKDKEKK FIWNHGITLP LKNVRKRRFR KTAKKKYIES P DVEKEVKR LLSTDAEAVS TRWEIIAEDE TKEAENQGLD ISSPGMSGHR QGHDSLEHDE LREIFNDLSS SSEDEDETQH QD EEDINII DTEEDLERQL QDKLNESDEQ HQENEGTNQL VMGIQKQIDN MKGKLQETQD RAKRQEDLIM KVENLALKNR FQA VLDELK QKEDREKEQL SSLQEELESL LEK

UniProtKB: Transcription initiation factor TFIID subunit 7

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Macromolecule #8: Transcription initiation factor TFIID subunit 8, TAF8

MacromoleculeName: Transcription initiation factor TFIID subunit 8, TAF8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 32.975344 KDa
SequenceString: MADAAATAGA GGSGTRSGSK QSTNPADNYH LARRRTLQVV VSSLLTEAGF ESAEKASVET LTEMLQSYIS EIGRSAKSYC EHTARTQPT LSDIVVTLVE MGFNVDTLPA YAKRSQRMVI T(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) ...String:
MADAAATAGA GGSGTRSGSK QSTNPADNYH LARRRTLQVV VSSLLTEAGF ESAEKASVET LTEMLQSYIS EIGRSAKSYC EHTARTQPT LSDIVVTLVE MGFNVDTLPA YAKRSQRMVI T(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)YQVLR EKAASQRRDV ERALTRFMAK TGETQSLFK DDVSTFPLIA ARPFTIPYLT ALLPSELEMQ QMEETDSSEQ DEQTDTENLA LHISMEDSGA EKENTSVLQQ N PSLSGSRN GEENIIDNPY LRPVKKPKIR RKKSLS

UniProtKB: Transcription initiation factor TFIID subunit 8

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Macromolecule #9: Transcription initiation factor TFIID subunit 9, TAF9

MacromoleculeName: Transcription initiation factor TFIID subunit 9, TAF9 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 28.830689 KDa
SequenceString: MESGKTASPK SMPKDAQMMA QILKDMGITE YEPRVINQML EFAFRYVTTI LDDAKIYSSH AKKATVDADD VRLAIQCRAD QSFTSPPPR DFLLDIARQR NQTPLPLIKP (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)PDR YCLTAPNYRL KSL QKKAST ...String:
MESGKTASPK SMPKDAQMMA QILKDMGITE YEPRVINQML EFAFRYVTTI LDDAKIYSSH AKKATVDADD VRLAIQCRAD QSFTSPPPR DFLLDIARQR NQTPLPLIKP (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)PDR YCLTAPNYRL KSL QKKAST SAGRITVPRL SVGSVTSRPS TPTLGTPTPQ TMSVSTKVGT PMSLTGQRFT VQMPTSQSPA VKASIPATSA VQNV LINPS LIGSKNILIT TNMMSSQNTA NESSNALKRK REDDDDDDDD DDDYDNL

UniProtKB: Transcription initiation factor TFIID subunit 9

+
Macromolecule #10: Transcription initiation factor TFIID subunit 10

MacromoleculeName: Transcription initiation factor TFIID subunit 10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 21.731248 KDa
SequenceString: MSCSGSGADP EAAPASAASA PGPAPPVSAP AALPSSTAAE NKASPAGTAG GPGAGAAAGG TGPLAARAGE PAERRGAAPV SAGGAAPPE GAISNGVYVL PSAANGDVKP VVSSTPLVDF LMQLEDYTPT IPDAVTGYYL NRAGFEASDP RIIRLISLAA Q KFISDIAN ...String:
MSCSGSGADP EAAPASAASA PGPAPPVSAP AALPSSTAAE NKASPAGTAG GPGAGAAAGG TGPLAARAGE PAERRGAAPV SAGGAAPPE GAISNGVYVL PSAANGDVKP VVSSTPLVDF LMQLEDYTPT IPDAVTGYYL NRAGFEASDP RIIRLISLAA Q KFISDIAN DALQHCKMKG TASGSSRSKS KDRKYTLTME DLTPALSEYG INVKKPHYFT

UniProtKB: Transcription initiation factor TFIID subunit 10

+
Macromolecule #11: Transcription initiation factor TFIID subunit 12

MacromoleculeName: Transcription initiation factor TFIID subunit 12 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 17.948467 KDa
SequenceString:
MNQFGPSALI NLSNFSSIKP EPASTPPQGS MANSTAVVKI PGTPGAGGRL SPENNQVLTK KKLQDLVREV DPNEQLDEDV EEMLLQIAD DFIESVVTAA CQLARHRKSS TLEVKDVQLH LERQWNMWIP GFGSEEIRPY KKACTTEAHK QRMALIRKTT K K

UniProtKB: Transcription initiation factor TFIID subunit 12

+
Macromolecule #12: TATA-box-binding protein

MacromoleculeName: TATA-box-binding protein / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 37.729938 KDa
SequenceString: MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS ILEEQQRQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQ QQQQQQAVAA AAVQQSTSQQ ATQGTSGQAP QLFHSQTLTT APLPGTTPLY PSPMTPMTPI TPATPASESS G IVPQLQNI ...String:
MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS ILEEQQRQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQ QQQQQQAVAA AAVQQSTSQQ ATQGTSGQAP QLFHSQTLTT APLPGTTPLY PSPMTPMTPI TPATPASESS G IVPQLQNI VSTVNLGCKL DLKTIALRAR NAEYNPKRFA AVIMRIREPR TTALIFSSGK MVCTGAKSEE QSRLAARKYA RV VQKLGFP AKFLDFKIQN MVGSCDVKFP IRLEGLVLTH QQFSSYEPEL FPGLIYRMIK PRIVLLIFVS GKVVLTGAKV RAE IYEAFE NIYPILKGFR KTT

UniProtKB: TATA-box-binding protein

+
Macromolecule #15: Transcription initiation factor IIA subunit 1

MacromoleculeName: Transcription initiation factor IIA subunit 1 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 10.675194 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
TVPKLYRSVI EDVINDVRDI FLDDGVDEQV LMELKTLWEN KLMDTENVVV CQYDKIHRSK NKWKFHLKDG IMNLNGRDYI FSKAIGDAE W

+
Macromolecule #16: Transcription initiation factor IIA subunit 2

MacromoleculeName: Transcription initiation factor IIA subunit 2 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 11.275824 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
YQLYRNTTLG NSLQESLDEL IQSQQITPQL ALQVLLQFDK AINAALAQRV RNRVNFRGSL NTYRFCDNVW TFVLNDVEFR EVTELIKVD KVKIVACD

UniProtKB: Transcription initiation factor IIA subunit 2

+
Macromolecule #17: Unk

MacromoleculeName: Unk / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.272906 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

+
Macromolecule #13: SCP DNA (80-MER)

MacromoleculeName: SCP DNA (80-MER) / type: dna / ID: 13 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.498586 KDa
SequenceString: (DC)(DC)(DG)(DT)(DA)(DG)(DG)(DC)(DA)(DC) (DG)(DT)(DC)(DT)(DG)(DC)(DT)(DC)(DG)(DG) (DC)(DT)(DC)(DG)(DA)(DG)(DT)(DG)(DT) (DT)(DC)(DG)(DA)(DT)(DC)(DG)(DC)(DG)(DA) (DC) (DT)(DG)(DA)(DG)(DG)(DA) ...String:
(DC)(DC)(DG)(DT)(DA)(DG)(DG)(DC)(DA)(DC) (DG)(DT)(DC)(DT)(DG)(DC)(DT)(DC)(DG)(DG) (DC)(DT)(DC)(DG)(DA)(DG)(DT)(DG)(DT) (DT)(DC)(DG)(DA)(DT)(DC)(DG)(DC)(DG)(DA) (DC) (DT)(DG)(DA)(DG)(DG)(DA)(DC)(DG) (DA)(DA)(DC)(DG)(DC)(DG)(DC)(DC)(DC)(DC) (DC)(DA) (DC)(DC)(DC)(DC)(DC)(DT)(DT) (DT)(DT)(DA)(DT)(DA)(DG)(DG)(DC)(DG)(DC) (DC)(DC)(DT)

+
Macromolecule #14: SCP DNA (80-MER)

MacromoleculeName: SCP DNA (80-MER) / type: dna / ID: 14 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.854842 KDa
SequenceString: (DA)(DG)(DG)(DG)(DC)(DG)(DC)(DC)(DT)(DA) (DT)(DA)(DA)(DA)(DA)(DG)(DG)(DG)(DG)(DG) (DT)(DG)(DG)(DG)(DG)(DG)(DC)(DG)(DC) (DG)(DT)(DT)(DC)(DG)(DT)(DC)(DC)(DT)(DC) (DA) (DG)(DT)(DC)(DG)(DC)(DG) ...String:
(DA)(DG)(DG)(DG)(DC)(DG)(DC)(DC)(DT)(DA) (DT)(DA)(DA)(DA)(DA)(DG)(DG)(DG)(DG)(DG) (DT)(DG)(DG)(DG)(DG)(DG)(DC)(DG)(DC) (DG)(DT)(DT)(DC)(DG)(DT)(DC)(DC)(DT)(DC) (DA) (DG)(DT)(DC)(DG)(DC)(DG)(DA)(DT) (DC)(DG)(DA)(DA)(DC)(DA)(DC)(DT)(DC)(DG) (DA)(DG) (DC)(DC)(DG)(DA)(DG)(DC)(DA) (DG)(DA)(DC)(DG)(DT)(DG)(DC)(DC)(DT)(DA) (DC)(DG)(DG)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

Concentration.2 mg/mL
BufferpH: 7.9
Component:
ConcentrationFormula
10.0 mMHEPES
10.0 mMMgCl
1.0 mMDTT
3.0 %Trehalose
50.0 mMKCl
0.0125 %NP-40
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV / Details: BT 4s; BF 15N.

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Electron microscopy

MicroscopeFEI TITAN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm
Sample stageSpecimen holder model: GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER
Cooling holder cryogen: NITROGEN

+
Image processing

CTF correctionSoftware - Name: Gctf / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 7.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 25180
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION

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