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TitleStructure of human TFIID and mechanism of TBP loading onto promoter DNA.
Journal, issue, pagesScience, Vol. 362, Issue 6421, Year 2018
Publish dateDec 21, 2018
AuthorsAvinash B Patel / Robert K Louder / Basil J Greber / Sebastian Grünberg / Jie Luo / Jie Fang / Yutong Liu / Jeff Ranish / Steve Hahn / Eva Nogales /
PubMed AbstractThe general transcription factor IID (TFIID) is a critical component of the eukaryotic transcription preinitiation complex (PIC) and is responsible for recognizing the core promoter DNA and ...The general transcription factor IID (TFIID) is a critical component of the eukaryotic transcription preinitiation complex (PIC) and is responsible for recognizing the core promoter DNA and initiating PIC assembly. We used cryo-electron microscopy, chemical cross-linking mass spectrometry, and biochemical reconstitution to determine the complete molecular architecture of TFIID and define the conformational landscape of TFIID in the process of TATA box-binding protein (TBP) loading onto promoter DNA. Our structural analysis revealed five structural states of TFIID in the presence of TFIIA and promoter DNA, showing that the initial binding of TFIID to the downstream promoter positions the upstream DNA and facilitates scanning of TBP for a TATA box and the subsequent engagement of the promoter. Our findings provide a mechanistic model for the specific loading of TBP by TFIID onto the promoter.
External linksScience / PubMed:30442764 / PubMed Central
MethodsEM (single particle)
Resolution4.2 - 23.0 Å
Structure data

9298

6mzc

EMDB-9298, PDB-6mzc:
Human TFIID BC core
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-9299:
Human TFIID BC core
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-9300:
Human TFIID BC core
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-9301:
Human TFIID BC core
Method: EM (single particle) / Resolution: 10.3 Å

9302

6mzd

EMDB-9302, PDB-6mzd:
Human TFIID Lobe A canonical
Method: EM (single particle) / Resolution: 9.8 Å

9305

6mzl

EMDB-9305, PDB-6mzl:
Human TFIID canonical state
Method: EM (single particle) / Resolution: 23.0 Å

9306

6mzm

EMDB-9306, PDB-6mzm:
Human TFIID bound to promoter DNA and TFIIA
Method: EM (single particle) / Resolution: 7.5 Å

Source
  • homo sapiens (human)
  • Human (human)
KeywordsTRANSCRIPTION / DNA / Nuclear / transcription/dna / transcription-dna complex

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