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Open data
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Basic information
Entry | Database: EMDB / ID: 9285 |
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Title | MicroED map of cinchonine at 1.0 A resolution |
![]() | 2Fo-Fc map of cinchonine |
![]() | Cinchonine: |
Source | synthetic construct (others) |
Method | ![]() ![]() |
![]() | Jones CG / Martynowycz MW / Hattne J / Fulton T / Stoltz BM / Rodriguez JA / Nelson HM / Gonen T |
![]() | Journal: ACS Cent Sci / Year: 2018 Title: The CryoEM Method MicroED as a Powerful Tool for Small Molecule Structure Determination. ![]() |
Date | Deposition: Oct 30, 2018 / Header (metadata) release: Nov 7, 2018 / Map release: Nov 21, 2018 / Last update: Jan 2, 2019 |
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Structure visualization
Movie |
![]() |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
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Download
![]() | ![]() |
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Header (meta data in XML format) | ![]() ![]() ![]() |
Images | ![]() |
FTP directory | ftp://ftp.pdbj.org/pub/emdb/structures/EMD-9285 |
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Links
-Related structure data
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. (generated in cubic-lattice coordinate) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X: 0.2975 Å / Y: 0.29417 Å / Z: 0.30972 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire Cinchonine
Entire | Name: Cinchonine / Number of components: 1 |
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-Component #1: cellular-component, Cinchonine
Cellular-component | Name: Cinchonine / Recombinant expression: No |
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Mass | Theoretical: 0.272217 kDa |
Source | Species: synthetic construct (others) |
-Experimental details
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Sample preparation
Specimen | Specimen state: 3D array / Method: ![]() |
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Crystal parameters | Space group: P 21/n / A: 10.71 Å / B: 7.06 Å / C: 11.15 Å / Alpha: 90 deg. / Beta: 109.66 deg. / Gamma: 90 deg. |
Crystal grow details | Powder |
Sample solution | Specimen conc.: 1 mg/ml / Buffer solution: Powder / pH: 7 |
Support film | unspecified |
Vitrification | Cryogen name: NITROGEN / Temperature: 298 K / Humidity: 100 % / Details: Hand-plunged. |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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![]() | Microscope: FEI TALOS ARCTICA |
Electron gun | Electron source: FIELD EMISSION GUN![]() |
Lens | Imaging mode: DIFFRACTION![]() |
Specimen Holder | Model: FEI TITAN KRIOS AUTOGRID HOLDER |
Camera | Detector: FEI CETA (4k x 4k) |
-Image acquisition
Image acquisition | Sampling size: 28 microns / Details: FEI CetaD |
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Image processing
![]() | Method: ![]() |
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3D reconstruction | Resolution method: DIFFRACTION PATTERN/LAYERLINES |
-Atomic model buiding
Modeling #1 | Refinement space: RECIPROCAL |
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