|Entry||Database: EMDB / ID: 8899|
|Title||Negative stain reconstruction of human autophagy associated protein complex, ATG2A-WIPI4|
|Map data||Negative stain reconstruction of human ATG2A-WIPI4 complex|
|Sample||Complex between human ATG2A and WIPI4 proteins.:|
|Function / homology||WD40 repeat / WD40-repeat-containing domain superfamily / Autophagy-related protein 2 / WD40/YVTN repeat-like-containing domain superfamily / Autophagy-related, C-terminal / WD repeat domain phosphoinositide-interacting protein 4 / Autophagy-related protein 2 CAD motif / Vacuolar protein sorting-associated protein 13, N-terminal domain / WD domain, G-beta repeat / Autophagy-related protein C terminal domain ...WD40 repeat / WD40-repeat-containing domain superfamily / Autophagy-related protein 2 / WD40/YVTN repeat-like-containing domain superfamily / Autophagy-related, C-terminal / WD repeat domain phosphoinositide-interacting protein 4 / Autophagy-related protein 2 CAD motif / Vacuolar protein sorting-associated protein 13, N-terminal domain / WD domain, G-beta repeat / Autophagy-related protein C terminal domain / N-terminal region of Chorein or VPS13 / Autophagy-related protein 2 CAD motif / Macroautophagy / protein lipidation / autophagy of nucleus / protein localization to phagophore assembly site / phagophore assembly site membrane / phosphatidylinositol-3,5-bisphosphate binding / phosphatidylinositol-3-phosphate binding / phagophore assembly site / autophagosome assembly / lipid droplet / autophagy of mitochondrion / extrinsic component of membrane / autophagy / intracellular membrane-bounded organelle / nucleus / cytosol / Autophagy-related protein 2 homolog A / WD repeat domain phosphoinositide-interacting protein 4|
Function and homology information
|Source||Homo sapiens (human)|
|Method||single particle reconstruction / 30.3 Å resolution|
|Authors||Chowdhury S / Otomo C / Leitner A / Ohashi K / Aebersold R / Lander GC / Otomo T|
|Citation||Journal: To be published|
Title: To be Published
Authors: Chowdhury S / Otomo C / Leitner A / Ohashi K / Aebersold R / Lander GC / Otomo T
|Date||Deposition: Aug 21, 2017 / Header (metadata) release: Sep 6, 2017 / Map release: Sep 6, 2017 / Last update: Feb 14, 2018|
|Structure viewer||EM map: |
Downloads & links
|File||emd_8899.map.gz (map file in CCP4 format, 3539 KB)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 4.1 Å|
CCP4 map header:
-Entire Complex between human ATG2A and WIPI4 proteins.
|Entire||Name: Complex between human ATG2A and WIPI4 proteins. / Details: Full length proteins / Number of components: 1|
|Mass||Theoretical: 250 kDa|
-Component #1: protein, Complex between human ATG2A and WIPI4 proteins.
|Protein||Name: Complex between human ATG2A and WIPI4 proteins. / Details: Full length proteins / Recombinant expression: No|
|Mass||Theoretical: 250 kDa|
|Source||Species: Homo sapiens (human)|
|Source (engineered)||Expression System: Spodoptera frugiperda (fall armyworm) / Strain: 9|
|Specimen||Specimen state: particle|
|Sample solution||Specimen conc.: 0.01 mg/ml / pH: 7.5|
|Vitrification||Cryogen name: NONE|
-Electron microscopy imaging
Model: Tecnai Spirit / Image courtesy: FEI Company
|Imaging||Microscope: FEI TECNAI SPIRIT|
|Electron gun||Electron source: LAB6 / Accelerating voltage: 120 kV / Electron dose: 20 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Magnification: 52000 X (nominal), 52000 X (calibrated) / Cs: 2.2 mm / Imaging mode: BRIGHT FIELD / Defocus: 1000 - 1500 nm|
|Specimen Holder||Model: SIDE ENTRY, EUCENTRIC / Temperature: K ( 273.15 - 298.15 K)|
|Camera||Detector: TVIPS TEMCAM-F416 (4k x 4k)|
|Image acquisition||Number of digital images: 1407 / Sampling size: 15.6 microns|
|Processing||Method: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 5317|
|3D reconstruction||Software: RELION / Resolution: 30.3 Å / Resolution method: FSC 0.143 CUT-OFF|
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