|Entry||Database: EMDB / ID: 8578|
|Title||Negative Stain EM of C05 mutant Fab (VPGSGW) and CR9114 Fab in complex with H1 HA Trimer|
|Sample||Negative stain EM map of C05 Mutant Fab (VPGSGW) and CR9114 fabs in complex with H1 HA trimer.|
|Source||Influenza A virus (A/Solomon Islands/3/2006(H1N1)) / virus|
|Map data||EM negative stain map of CR9114 and VPGSGW fabs in complex with H1 hemagglutinin trimer.|
|Method||single particle reconstruction, at 18 Å resolution|
|Authors||Turner HL / Ward AB|
|Citation||Nat Commun, 2017, 8, 15371-15371|
|Date||Deposition: Feb 1, 2017 / Header (metadata) release: Feb 15, 2017 / Map release: May 31, 2017 / Last update: Feb 14, 2018|
Downloads & links
|File||emd_8578.map.gz (map file in CCP4 format, 11944 KB)|
|Projections & slices|
Images are generated by Spider package.
|Voxel size||X=Y=Z: 2.05 Å|
CCP4 map header:
-Entire Negative stain EM map of C05 Mutant Fab (VPGSGW) and CR9114 fabs ...
|Entire||Name: Negative stain EM map of C05 Mutant Fab (VPGSGW) and CR9114 fabs in complex with H1 HA trimer.|
Number of components: 1
-Component #1: protein, Negative stain EM map of C05 Mutant Fab (VPGSGW) and CR9...
|Protein||Name: Negative stain EM map of C05 Mutant Fab (VPGSGW) and CR9114 fabs in complex with H1 HA trimer.|
Recombinant expression: No
|Source||Species: Influenza A virus (A/Solomon Islands/3/2006(H1N1)) / virus|
Strain: A/Solomon Islands/3/2006(H1N1)
|Source (engineered)||Expression System: Drosophila / arthropod / image: Drosophila melanogaster|
|Sample solution||Specimen conc.: 0.019 mg/ml|
Buffer solution: Solution was made fresh using 0.2um filter.
|Staining||0.2% Uranyl Formate. Samples were placed on 400 mesh copper grids covered with nitrocellulose. Samples were blotted off and UF was added for a total of 30 seconds before being blotted off.|
|Vitrification||Cryogen name: NONE|
-Electron microscopy imaging
Model: Tecnai Spirit / Image courtesy: FEI Company
|Imaging||Microscope: FEI TECNAI SPIRIT|
|Electron gun||Electron source: LAB6 / Accelerating voltage: 120 kV / Electron dose: 25 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Imaging mode: BRIGHT FIELD|
|Specimen Holder||Model: OTHER|
|Camera||Detector: TVIPS TEMCAM-F416 (4k x 4k)|
|Image acquisition||Number of digital images: 93|
|Processing||Method: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 18874|
|3D reconstruction||Algorithm: BACK PROJECTION / Software: sxali3d.py / Resolution: 18 Å / Resolution method: FSC 0.5 CUT-OFF|
-Oct 4, 2017. Three pioneers of this field were awarded Nobel Prize in Chemistry 2017
Three pioneers of this field were awarded Nobel Prize in Chemistry 2017
- Jacques Dubochet (University of Lausanne, Switzerland) is a pioneer of ice-embedding method of EM specimen (as known as cryo-EM), Most of 3DEM structures in EMDB and PDB are obtained using his method.
- Joachim Frank (Columbia University, New York, USA) is a pioneer of single particle reconstruction, which is the most used reconstruction method for 3DEM structures in EMDB and EM entries in PDB. And also, he is a develper of Spider, which is one of the most famous software in this field, and is used for some EM Navigor data (e.g. map projection/slice images).
- Richard Henderson (MRC Laboratory of Molecular Biology, Cambridge, UK) was determined the first biomolecule structure by EM. The first EM entry in PDB, PDB-1brd is determinedby him.
External links: The 2017 Nobel Prize in Chemistry - Press Release
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