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- EMDB-8123: Structure of the Kluyveromyces lactis 80S ribosome in complex wit... -

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Basic information

Entry
Database: EMDB / ID: EMD-8123
TitleStructure of the Kluyveromyces lactis 80S ribosome in complex with the cricket paralysis virus IRES and eEF2
Map dataNone
SampleeEF2 != Kluyveromyces lactis 80S ribosome in complex with the cricket paralysis virus IRES and eEF2

eEF2

  • Complex: Kluyveromyces lactis 80S ribosome in complex with the cricket paralysis virus IRES and eEF2
    • Other: x 2 types
    • RNA: x 3 types
    • Protein or peptide: x 78 types
  • Complex: Kluyveromyces lactis 80S ribosome
  • Complex: Cricket paralysis virus IRES
  • Complex: eEF2
  • Ligand: x 4 types
Keywordsribosome / translocation / IRES / eEF2
Function / homology
Function and homology information


response to cycloheximide / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / 90S preribosome / protein-RNA complex assembly / translation regulator activity ...response to cycloheximide / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / 90S preribosome / protein-RNA complex assembly / translation regulator activity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / rRNA processing / ribosome biogenesis / large ribosomal subunit / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / response to antibiotic / mRNA binding / nucleolus / RNA binding / zinc ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / 50S ribosomal protein L10, insertion domain superfamily / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / : / : / Ribosomal protein S26e signature. / Ribosomal protein L41 ...60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / 50S ribosomal protein L10, insertion domain superfamily / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / : / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein L1, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein L1 signature. / : / Ribosomal protein S12e signature. / Ribosomal protein L1 / Ribosomal protein S12e / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein S2, eukaryotic / S27a-like superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L10e / 40S Ribosomal protein S10 / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / : / Ribosomal protein S7e signature. / Ribosomal protein L44e signature. / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L19, eukaryotic / Ribosomal protein L13e / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein L13e / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S27a / Ribosomal protein S17e, conserved site / : / Ribosomal protein S27a / Ribosomal protein S17e signature. / Ribosomal protein S27a / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / Ribosomal protein L44e / Ribosomal protein S30 / Ribosomal protein L44 / Ribosomal protein S30 / Ribosomal protein S2, eukaryotic/archaeal / : / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L30e signature 1. / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal L40e family / Ribosomal protein S27e signature. / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein 60S L18 and 50S L18e / Ribosomal Protein L6, KOW domain / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L30e signature 2. / Ribosomal protein L27e / Ribosomal protein S19e
Similarity search - Domain/homology
60S ribosomal protein L8 / Large ribosomal subunit protein eL39 / Small ribosomal subunit protein eS32A / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein eL42 / Small ribosomal subunit protein eS28 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Ubiquitin-ribosomal protein eS31 fusion protein ...60S ribosomal protein L8 / Large ribosomal subunit protein eL39 / Small ribosomal subunit protein eS32A / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein eL42 / Small ribosomal subunit protein eS28 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Ubiquitin-ribosomal protein eS31 fusion protein / KLLA0F25542p / KLLA0F18040p / Ribosomal protein L15 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS5 / KLLA0F08261p / KLLA0F07843p / KLLA0F05247p / KLLA0F04675p / KLLA0F04499p / 40S ribosomal protein S12 / Small ribosomal subunit protein eS6 / KLLA0E23673p / KLLA0E23651p / 40S ribosomal protein S8 / Small ribosomal subunit protein uS2 / KLLA0E12453p / KLLA0E12277p / 40S ribosomal protein S27 / KLLA0E06997p / KLLA0E06843p / KLLA0E05941p / Small ribosomal subunit protein uS14 / KLLA0E03455p / 60S ribosomal protein L29 / KLLA0D16027p / 60S ribosomal protein L36 / KLLA0D10659p / 40S ribosomal protein S3 / KLLA0D07405p / KLLA0D06941p / KLLA0D05643p / KLLA0D05181p / 40S ribosomal protein S26 / KLLA0D03410p / KLLA0C18216p / 40S ribosomal protein S7 / KLLA0C08371p / 40S ribosomal protein S24 / 40S ribosomal protein S30 / Ribosomal protein L37 / KLLA0B13409p / KLLA0B08173p / Small ribosomal subunit protein uS8 / KLLA0B07139p / 40S ribosomal protein S25 / 60S acidic ribosomal protein P0 / KLLA0B05742p / Small ribosomal subunit protein eS1 / KLLA0B04686p / 40S ribosomal protein S4 / KLLA0B02937p / Ribosomal protein / KLLA0B01562p / KLLA0B01474p / KLLA0A10483p / KLLA0A07227p / KLLA0A07194p / KLLA0A06336p / Small ribosomal subunit protein eS21 / 60S ribosomal protein L13 / RPS23 / Small ribosomal subunit protein uS9 / RPL28 / Ubiquitin fusion protein / :
Similarity search - Component
Biological speciesKluyveromyces lactis (yeast) / Cricket paralysis virus / Saccharomyces cerevisiae P283 (yeast) / Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsMurray J / Savva CG
Funding support United Kingdom, United States, 3 items
OrganizationGrant numberCountry
Medical Research Council (United Kingdom)MC_U105184332 United Kingdom
Wellcome TrustWT096570 United Kingdom
National Institutes of HealthIntramural Research Program United States
CitationJournal: Elife / Year: 2016
Title: Structural characterization of ribosome recruitment and translocation by type IV IRES.
Authors: Jason Murray / Christos G Savva / Byung-Sik Shin / Thomas E Dever / V Ramakrishnan / Israel S Fernández /
Abstract: Viral mRNA sequences with a type IV IRES are able to initiate translation without any host initiation factors. Initial recruitment of the small ribosomal subunit as well as two translocation steps ...Viral mRNA sequences with a type IV IRES are able to initiate translation without any host initiation factors. Initial recruitment of the small ribosomal subunit as well as two translocation steps before the first peptidyl transfer are essential for the initiation of translation by these mRNAs. Using electron cryomicroscopy (cryo-EM) we have structurally characterized at high resolution how the Cricket Paralysis Virus Internal Ribosomal Entry Site (CrPV-IRES) binds the small ribosomal subunit (40S) and the translocation intermediate stabilized by elongation factor 2 (eEF2). The CrPV-IRES restricts tvhe otherwise flexible 40S head to a conformation compatible with binding the large ribosomal subunit (60S). Once the 60S is recruited, the binary CrPV-IRES/80S complex oscillates between canonical and rotated states (Fernández et al., 2014; Koh et al., 2014), as seen for pre-translocation complexes with tRNAs. Elongation factor eEF2 with a GTP analog stabilizes the ribosome-IRES complex in a rotated state with an extra ~3 degrees of rotation. Key residues in domain IV of eEF2 interact with pseudoknot I (PKI) of the CrPV-IRES stabilizing it in a conformation reminiscent of a hybrid tRNA state. The structure explains how diphthamide, a eukaryotic and archaeal specific post-translational modification of a histidine residue of eEF2, is involved in translocation.
History
DepositionApr 13, 2016-
Header (metadata) releaseMay 18, 2016-
Map releaseMay 18, 2016-
UpdateApr 9, 2025-
Current statusApr 9, 2025Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.14
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  • Surface view colored by height
  • Surface level: 0.14
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  • Surface view with fitted model
  • Atomic models: PDB-5it7
  • Surface level: 0.14
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Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_8123.png

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Map

FileDownload / File: emd_8123.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationNone
Projections & slices

Image control

Size
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.34 Å/pix.
x 320 pix.
= 428.8 Å		1.34 Å/pix.
x 320 pix.
= 428.8 Å		1.34 Å/pix.
x 320 pix.
= 428.8 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.34 Å
Density

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Histogram (log scale)
Contour LevelBy AUTHOR: 0.1 / Movie #1: 0.14
Minimum - Maximum-0.5777603 - 0.7931435
Average (Standard dev.)0.00028963832 (±0.043949272)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 428.80002 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.341.341.34
M x/y/z320320320
origin x/y/z0.0000.0000.000
length x/y/z428.800428.800428.800
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS320320320
D min/max/mean-0.5780.7930.000

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Supplemental data

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Mask #1

Fileemd_8123_msk_1.map
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Mask #2

Fileemd_8123_msk_2.map
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Additional map: None

Fileemd_8123_additional_1.map
AnnotationNone
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Additional map: None

Fileemd_8123_additional_2.map
AnnotationNone
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Half map: None

Fileemd_8123_half_map_1.map
AnnotationNone
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Half map: None

Fileemd_8123_half_map_2.map
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Sample components

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Entire : eEF2

EntireName: eEF2
Components
  • Complex: Kluyveromyces lactis 80S ribosome in complex with the cricket paralysis virus IRES and eEF2
    • Other: 25S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • RNA: 5.8S ribosomal RNA
    • Protein or peptide: KLLA0D16027p
    • Protein or peptide: 60S ribosomal protein L3
    • Protein or peptide: KLLA0B07139p
    • Protein or peptide: KLLA0D06941p
    • Protein or peptide: KLLA0B04686p
    • Protein or peptide: KLLA0D03410p
    • Protein or peptide: KLLA0E00573p
    • Protein or peptide: KLLA0F04499p
    • Protein or peptide: KLLA0D05643p
    • Protein or peptide: KLLA0F08261p
    • Protein or peptide: 60S ribosomal protein L13
    • Protein or peptide: KLLA0B13409p
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: KLLA0F04675p
    • Protein or peptide: KLLA0A06336p
    • Protein or peptide: KLLA0A07227p
    • Protein or peptide: KLLA0E12453p
    • Protein or peptide: 60S ribosomal protein L20
    • Protein or peptide: KLLA0E23651p
    • Protein or peptide: KLLA0D05181p
    • Protein or peptide: KLLA0E06997p
    • Protein or peptide: 60S ribosomal protein L24
    • Protein or peptide: 60S ribosomal protein L25
    • Protein or peptide: KLLA0B05742p
    • Protein or peptide: KLLA0E03455p
    • Protein or peptide: RPL28
    • Protein or peptide: KLLA0D16071p
    • Protein or peptide: 60S ribosomal protein L30
    • Protein or peptide: KLLA0B02937p
    • Protein or peptide: KLLA0E06843p
    • Protein or peptide: KLLA0D07405p
    • Protein or peptide: KLLA0C08371p
    • Protein or peptide: KLLA0F05247p
    • Protein or peptide: 60S ribosomal protein L36
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: KLLA0C18216p
    • Protein or peptide: 60S ribosomal protein L39
    • Protein or peptide: Ubiquitin fusion protein
    • Protein or peptide: 60S ribosomal protein L41-A
    • Protein or peptide: 60S ribosomal protein L44
    • Protein or peptide: KLLA0E05941p
    • Protein or peptide: Ribosomal protein
    • Protein or peptide: 60S acidic ribosomal protein P0
    • Protein or peptide: uL11
    • Protein or peptide: 40S ribosomal protein S0
    • Protein or peptide: 40S ribosomal protein S1
    • Protein or peptide: KLLA0F09812p
    • Protein or peptide: KLLA0D08305p
    • Protein or peptide: 40S ribosomal protein S4
    • Protein or peptide: KLLA0D10659p
    • Protein or peptide: 40S ribosomal protein S6
    • Protein or peptide: KLLA0C13519p
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: KLLA0E23673p
    • Protein or peptide: KLLA0B08173p
    • Protein or peptide: KLLA0A10483p
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: KLLA0F18040p
    • Protein or peptide: 40S ribosomal protein S14
    • Protein or peptide: KLLA0F07843p
    • Protein or peptide: 40S ribosomal protein S16
    • Protein or peptide: KLLA0B01474p
    • Protein or peptide: KLLA0B01562p
    • Protein or peptide: KLLA0A07194p
    • Protein or peptide: KLLA0F25542p
    • Protein or peptide: 40S ribosomal protein S21
    • Protein or peptide: 40S ribosomal protein S22
    • Protein or peptide: RPS23
    • Protein or peptide: 40S ribosomal protein S24
    • Protein or peptide: KLLA0B06182p
    • Protein or peptide: KLLA0D05115p
    • Protein or peptide: 40S ribosomal protein S27
    • Protein or peptide: 40S ribosomal protein S28
    • Protein or peptide: 40S ribosomal protein S29
    • Protein or peptide: KLLA0C04809p
    • Protein or peptide: Ubiquitin-40S ribosomal protein S27a
    • Protein or peptide: KLLA0E12277p
    • RNA: 18S ribosomal RNA
    • Other: cricket paralysis virus IRES
    • Protein or peptide: Eft2p
  • Complex: Kluyveromyces lactis 80S ribosome
  • Complex: Cricket paralysis virus IRES
  • Complex: eEF2
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
  • Ligand: (2S)-1-amino-N,N,N-trimethyl-1-oxobutan-2-aminium

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Supramolecule #1: Kluyveromyces lactis 80S ribosome in complex with the cricket par...

SupramoleculeName: Kluyveromyces lactis 80S ribosome in complex with the cricket paralysis virus IRES and eEF2
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#83
Molecular weightTheoretical: 4 MDa

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Supramolecule #2: Kluyveromyces lactis 80S ribosome

SupramoleculeName: Kluyveromyces lactis 80S ribosome / type: complex / ID: 2 / Parent: 0 / Macromolecule list: #1-#81
Source (natural)Organism: Kluyveromyces lactis (yeast)

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Supramolecule #3: Cricket paralysis virus IRES

SupramoleculeName: Cricket paralysis virus IRES / type: complex / ID: 3 / Parent: 0 / Macromolecule list: #82
Source (natural)Organism: Cricket paralysis virus

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Supramolecule #4: eEF2

SupramoleculeName: eEF2 / type: complex / ID: 4 / Parent: 0 / Macromolecule list: #83
Source (natural)Organism: Saccharomyces cerevisiae P283 (yeast)

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Macromolecule #1: 25S ribosomal RNA

MacromoleculeName: 25S ribosomal RNA / type: other / ID: 1 / Number of copies: 1
Classification: polydeoxyribonucleotide/polyribonucleotide hybrid
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 1.0567063749999999 MDa
SequenceString: UUGACCUCAA AUCAGGUAGG AAUACCCGCU GAACUUAAGC AUAUCAAUAA GCGGAGGAAA AGAAACCAAC CGGGAUUGCC UUAGUAACG GCGAGUGAAG CGGCAAAAGC UCAAAUUUGA AAUCUGGCGU CUUCGACGUC CGAGUUGUAA UUUGAAGAAG G CAACUUUG ...String:
UUGACCUCAA AUCAGGUAGG AAUACCCGCU GAACUUAAGC AUAUCAAUAA GCGGAGGAAA AGAAACCAAC CGGGAUUGCC UUAGUAACG GCGAGUGAAG CGGCAAAAGC UCAAAUUUGA AAUCUGGCGU CUUCGACGUC CGAGUUGUAA UUUGAAGAAG G CAACUUUG UAGCUGGUCC UUGUCUAUGU UCCUUGGAAC AGGACGUCAU AGAGGGUGAG AAUCCCGUGU GGCGAGGAUC CC AGUUAUU UGUAAAGUGC UUUCGACGAG UCGAGUUGUU UGGGAAUGCA GCUCUAAGUG GGUGGUAAAU UCCAUCUAAA GCU AAAUAU UGGCGAGAGA CCGAUAGCGA ACAAGUACAG UGAUGGAAAG AUGAAAAGAA CUUUGAAAAG AGAGUGAAAA AGUA CGUGA AAUUGUUGAA AGGGAAGGGC AUUUGAUCAG ACAUGGGCCA GCAUCAGUUU UAGCGGUUGG AUAAAUCCUC GGGAA UGUG GCUCUGCUUC GGUAGAGUGU UAUAGCCCGU GGGAAUACAG CCAGCUGGGA CUGAGGAUUG CGACUUUUGU CAAGGA UGC UGGCGUAAUG GUUAAAUGCC GCCCGUCUUG AAACACGGAC CAAGGAGUCU AACGUCUAUG CGAGUGUUUG GGUGUAA AA CCCGUACGCG UAAUGAAAGU GAACGUAGGU GAGGGCCCGC AAGGGUGCAU CAUCGACCGA UCCUGAUGUC UUCGGAUG G AUUUGAGUAA GAGCAUAGCU GUUGGGACCC GAAAGAUGGU GAACUAUGCC UGAAUAGGGU GAAGCCAGAG GAAACUCUG GUGGAGGCUC GUAGCGGUUC UGACGUGCAA AUCGAUCGUC GAAUUUGGGU AUAGGGGCGA AAGACUAAUC GAACCAUCUA GUAGCUGGU UCCUGCCGAA GUUUCCCUCA GGAUAGCAGA AGCUCGUAUC AGUUUUAUGA GGUAAAGCGA AUGAUUAGAG G UACCGGGG UUGAAAUGAC CUUGACCUAU UCUCAAACUU UAAAUAUGUA AGAAGUCCUU GUUGCUUAAU UGAACGUGGA CA UUUGAAU GAAGAGCUUU UAGUGGGCCA UUUUUGGUAA GCAGAACUGG CGAUGCGGGA UGAACCGAAC GUGGAGUUAA GGU GCCGGA AUACACGCUC AUCAGACACC ACAAAAGGUG UUAGUUCAUC UAGACAGCCG GACGGUGGCC AUGGAAGUCG GAAU CCGCU AAGGAGUGUG UAACAACUCA CCGGCCGAAU GAACUAGCCC UGAAAAUGGA UGGCGCUCAA GCGUGUUACC UAUAC UCCA CCGUCAGGGU UAAUAUGAUG CCCUGACGAG UAGGCAGGCG UGGAGGUCAG UGACGAAGCC UAGGCUGUAA AGCUGG GUA GAACGGCCUC UAGUGCAGAU CUUGGUGGUA GUAGCAAAUA UUCAAAUGAG AACUUUGAAG ACUGAAGUGG GGAAAGG UU CCACGUCAAC AGCAGUUGGA CGUGGGUUAG UCGAUCCUAA GAGAUGGGGA AGCUCCGUUU CAAAGGCCUA AUUUUCUA G GCCACCAUCG AAAGGGAAUC CGGUUAAUAU UCCGGAACCU GGAUAUGGAU UCUUCACGGU AACGUAACUG AAUGUGGAG ACGUCGGCGC GAGCCCUGGG AGGAGUUAUC UUUUCUUCUU AACAGCUUAU CACCCCGGAA UUGGUUUAUC CGGAGAGGGG GUCUUAUGG CUGGAAGAGC CCAGCCCUUG UGCUGGGUCC GGUGCGCCCG CGACGGCCCU UGAAAAUCCA CAGGAAGGAA U AGUUUUCA UGCCAGGUCG UACUGAUAAC CGCAGCAGGU CUCCAAGGUG AACAGCCUCU AGUUGAUAGA AUAAUGUAGA UA AGGGAAG UCGGCAAAAU AGAUCCGUAA CUUCGGGAUA AGGAUUGGCU CUAAGGAUCG GGCGUGAG(DG)G CCUUGGUCAG ACGCGGCGU UGUAGACGGC CUUGGU(DA)GGU CUCUUGUAGA UGCUGCAAUU AACGAUCAAC UUAGAACUGG UACGGACA A GGGGAAUCUG ACUGUCUAAU UAAAACAUAG CAUUGCGAUG GUCAGAAAGU GAUGUUGACG CAAUGUGAUU UCUGCCCAG UGCUCUGAAU GUCAAAGUGA AGAAAUUCAA CCAAGCGCGG GUAAACGGCG GGAGUAACUA UGACUCUCUU AAGGUAGCCA AAUGCCUCG UCAUCUAAUU AGUGACGCGC AUGAAUGGAU UAACGAGAUU CCCACUGUCC CUAUCUACUA UCUAGCGAAA C CACAGCCA AGGGAACGGG CUUGGCAGAA UCAGCGGGGA AAGAAGACCC UGUUGAGCUU GACUCUAGUU UGACAUUGUG AA GAGACAU AGAGGGUGUA GCAUAAGUGG GAGCUUCGGC GCCAGUGAAA UACCACUACC UUUAUAGUUU CUUUACUUAU UCA AUUAAG CGGAGCUGGA AUUCAUUUUC CACGUUCUAG CAUUCAAAGU CCUAUACGGG CUGAUCCGGG UUGAAGACAU UGUC AGGUG GGGAGUUUGG CUGGGGCGGC ACAUCUGUUA AACGAUAACG CAGAUGUCCU AAGGGGGACU CAUGGAGAAC AGAAA UCUC CAGUAGAACA AAAGGGUAAA AGUCCCCUUG AUUUUGAUUU UCAGUGUGAA UACAAACCAU GAAAGUGUGG CCUAUC GAU CCUUUAGGUC CUCGGAAUUU GAGGCUAGAG GUGCCAGAAA AGUUACCACA GGGAUAACUG GCUUGUGGCA GUCAAGC GU UCAUAGCGAC AUUGCUUUUU GAUUCUUCGA UGUCGGCUCU UCCUAUCAUA CCGAAGCAGA AUUCGGUAAG CGUUGGAU U GUUCACCCAC UAAUAGGGAA CGUGAGCUGG GUUUAGACCG UCGUGAGACA GGUUAGUUUU ACCCUACUGA UGAAUGUUA UCGCAAUAGU AAUUGAACUU AGUACGAGAG GAACAGUUCA UUCGGAUAAU UGGUUUUUGC GGCUGUCUGA UCAGGCAUUG CCGCGAAGC UACCAUCCGC UGGAUUAUGG CUGAACGCCU CUAAGUCAGA AUCCAUGCUA GAACGCGAUG AUUUCUUUGC C UUGCACAA UAUAGAAGGA UAAGAAUAAG GCGUCUUUUU GGCGUCGCUG AACCAUAGCA GGCUGGCAAC GGUGCUCUUA GC GGAAAGG CUUUGGGUGC UUGCCGGCGA AUUGCAAUGU CAUUUUGCGC AAGGAUAAAU CAUUUGUAUA CGACUUAAAU GUA CAACAG GGUAUUGUAA GCAGUAGAGU AGCCUUGUUG UUACGAUCUG CUGAGAUUAA GCCUUCGUUG UCUGAUUUGU

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Macromolecule #82: cricket paralysis virus IRES

MacromoleculeName: cricket paralysis virus IRES / type: other / ID: 82 / Number of copies: 1
Classification: polydeoxyribonucleotide/polyribonucleotide hybrid
Source (natural)Organism: Cricket paralysis virus
Molecular weightTheoretical: 60.732805 KDa
SequenceString: AAAAAUGUGA UCUUGCUUGU AAAUACAAUU UUGAGAGGUU AAUAAAUUAC AAGUAGUGCU AUUUUUGUAU UUAGGUUAGC UAUUUAGCU UUACGUUCCA GGAUGCCUAG UGGCAGCCCC ACAAUAUCCA GGAAGCCCUC UCUACGGUUU UUCAGAUUAG G UAGUCGAA ...String:
AAAAAUGUGA UCUUGCUUGU AAAUACAAUU UUGAGAGGUU AAUAAAUUAC AAGUAGUGCU AUUUUUGUAU UUAGGUUAGC UAUUUAGCU UUACGUUCCA GGAUGCCUAG UGGCAGCCCC ACAAUAUCCA GGAAGCCCUC UCUACGGUUU UUCAGAUUAG G UAGUCGAA AAACCUA(DA)(DG)(DA) (DA)(DA)UUUACCUG CU
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Macromolecule #2: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 38.951105 KDa
SequenceString:
GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGG UGACCAUACG CGAAACUCAG GUGCUGCAAU CU

GENBANK: GENBANK: CP006425.1

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Macromolecule #3: 5.8S ribosomal RNA

MacromoleculeName: 5.8S ribosomal RNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 50.293609 KDa
SequenceString:
AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAUUGCGAU AUGUAUUGUG AAUUGCAGAU UUUCGUGAA UCAUCAAAUC UUUGAACGCA CAUUGCGCCC UCUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUU

GENBANK: GENBANK: KM384212.1

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Macromolecule #81: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 81 / Number of copies: 1
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 579.239625 KDa
SequenceString: UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGAUAUCU GUGGUAAUUC U AGAGCUAA ...String:
UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGAUAUCU GUGGUAAUUC U AGAGCUAA UACAUGCUUA AAAUCUCGAC CCUUUGGAAG AGAUGUAUUU AUUAGAUAAA AAAUCAAUGU CUUCGGACUC CU UGAUGAU UCAUAAUAAC UUUUCGAAUC GCAUGGCCUU GUGCUGGCGA UGGUUCAUUC AAAUUUCUGC CCUAUCAACU UUC GAUGGU AGGAUAGUGG CCUACCAUGG UUUCAACGGG UAACGGGGAA UAAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAA CGGCU ACCACAUCCA AGGAAGGCAG CAGGCGCGCA AAUUACCCAA UCCUAAUUCA GGGAGGUAGU GACAAUAAAU AACGA UACA GGGCCCAUUC GGGUCUUGUA AUUGGAAUGA GUACAAUGUA AAUACCUUAA CGAGGAACAA CUGGAGGGCA AGUCUG GUG CCAGCAGCCG CGGUAAUUCC AGCUCCAGUA GCGUAUAUUA AAGUUGUUGC AGUUAAAAAG CUCGUAGUUG AACUUUG GG UCUGGUUGUC CGGUCCGACU UUAUGUCGCG CACGGUUUUU CAACCGGAUC UUUCCUUCUG GCUAACCUGU ACUCCUUG U GGGUGCAGGC GAACCAGGAC UUUUACUUUG AAAAAAUUAG AGUGUUCAAA GCAGGCGAAA GCUCGAAUAU AUUAGCAUG GAAUAAUGGA AUAGGACGUU UGGUUCUAUU UUGUUGGUUU CUAGGACCAU CGUAAUGAUU AAUAGGGACG GUCGGGGGCA UCAGUAUUC AAUUGUCAGA GGUGAAAUUC UUGGAUUUAU UGAAGACUAA CUACUGCGAA AGCAUUUGCC AAGGACGUUU U CAUUAAUC AAGAACGAAA GUUAGGGGAU CGAAGAUGAU CAGAUACCGU CGUAGUCUUA ACCAUAAACU AUGCCGACUA GG GAUCGGG UGGUGUUUUU CUUAUGACCC ACUCGGCACC UUACGAGAAA UCAAAGUCUU UGGGUUCUGG GGGGAGUAUG GUC GCAAGG CUGAAACUUA AAGGAAUUGA CGGAAGGGCA CCACCAGGAG UGGAGCCUGC GGCUUAAUUU GACUCAACAC GGGG AAACU CACCAGGUCC AGACACAAUA AGGAUUGACA GAUUGAGAGC UCUUUCUUGA UUUUGUGGGU GGUGGUGCAU GGCCG UUCU UAGUUGGUGG AGUGAUUUGU CUGCUUAAUU GCGAUAACGA ACGAGACCUU AACCUACUAA AUAGGGUUGC UGGCAC UUG CCGGUUGACU CUUCUUAGAG GGACUAUCGG UUUCAAGCCG AUGGAAGUUU GAGGCAAUAA CAGGUCUGUG AUGCCCU UA GACGUUCUGG GCCGCACGCG CGCUACACUG ACGGAGCCAG CGAGUACAAC CUUGGCCGAG AGGUCUGGGU AAUCUUGU G AAACUCCGUC GUGCUGGGGA UAGAGCAUUG UAAUUAUUGC UCUUCAACGA GGAAUUCCUA GUAAGCGCAA GUCAUCAGC UUGCGUUGAU UACGUCCCUG CCCUUUGUAC ACACCGCCCG UCGCUAGUAC CGAUUGAAUG GCUUAGUGAG GCCUCAGGAU UUGCUUAGA GAAGGGGGCA ACUCCAUCUC AGAGCGAAGA AUCUGGUCAA ACUUGGUCAU UUAGAGGAAC UAAAAGUCGU A ACAAGGUU UCCGUAGGUG AACCUGCGGA AGGAUCAUUA

GENBANK: GENBANK: CR382124.1

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Macromolecule #4: KLLA0D16027p

MacromoleculeName: KLLA0D16027p / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 26.843818 KDa
SequenceString: GRVIRNQRKG AGSIFTSHTR LRQGAAKLRN LDYAERHGYI RGVVKQIVHD AGRGAPLAKV VFRDPYKYKL REETFIANEG LHTGQFIYA GKKASLNVGN VLPLGSVPEG TIISNVEERP GDRGALARAS GNYVIVIGHN PEDNKTRVRL PSGAKKIISS D ARGVIGVV ...String:
GRVIRNQRKG AGSIFTSHTR LRQGAAKLRN LDYAERHGYI RGVVKQIVHD AGRGAPLAKV VFRDPYKYKL REETFIANEG LHTGQFIYA GKKASLNVGN VLPLGSVPEG TIISNVEERP GDRGALARAS GNYVIVIGHN PEDNKTRVRL PSGAKKIISS D ARGVIGVV AGGGRIDKPL LKAGRAFHKY RVKRNSWPKT RGVAMNPVDH PHGGGNHQHI GKASTISRGA VSGQKAGLIA AR RTGLLRG SQ

UniProtKB: KLLA0D16027p

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Macromolecule #5: 60S ribosomal protein L3

MacromoleculeName: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 43.422066 KDa
SequenceString: SHRKYEAPRH GHLGFLPRKR AASVRGRVKS FPKDDQTKPV ALTSFLGYKA GMSTIVRDLD RPGSKFHKRE VVEAVTVVDT PPIVVVGVV GYVETPRGLR SLTTVWAEHL SDEVKRRFYK NWYKSKKKAF TKYSAKYAEN GAQVDRELAR IKKYASVVRV L VHTQVRKT ...String:
SHRKYEAPRH GHLGFLPRKR AASVRGRVKS FPKDDQTKPV ALTSFLGYKA GMSTIVRDLD RPGSKFHKRE VVEAVTVVDT PPIVVVGVV GYVETPRGLR SLTTVWAEHL SDEVKRRFYK NWYKSKKKAF TKYSAKYAEN GAQVDRELAR IKKYASVVRV L VHTQVRKT PLSQKKAHLA EIQLNGGSVS DKVDWAKEHF EKTVAVDSVF EQNEMIDVVA VTKGHGFEGV THRWGTKKLP RK THRGLRK VACIGAWHPA HVMWSVARAG QRGYHHRTSI NHKVYRVGKG DDEANAATEF DRTKKTITPM GGFVHYGAIN NDF VILKGS IPGTRKRVVT LRKSLYTNTS RKALEEVTLK WIDTASKFGK GRFQTPAEKH AFLGTLKK

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #6: KLLA0B07139p

MacromoleculeName: KLLA0B07139p / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 38.993902 KDa
SequenceString: RPQVAVLSLN GETTSTDLPL PAVFRAPIRP DIVHSVFTSV NKNKRQAYAV SEKAGHQTSA ESWGTGRAVA RIPRVGGGGT HRSGQAAFG NMCRGGRMFA PTKTWRKWNV KVNQNEKRYA TASAIAASAV PSLVLARGHR VEKVPSIPLV VSDDLQSIQK T KEAVAALK ...String:
RPQVAVLSLN GETTSTDLPL PAVFRAPIRP DIVHSVFTSV NKNKRQAYAV SEKAGHQTSA ESWGTGRAVA RIPRVGGGGT HRSGQAAFG NMCRGGRMFA PTKTWRKWNV KVNQNEKRYA TASAIAASAV PSLVLARGHR VEKVPSIPLV VSDDLQSIQK T KEAVAALK AVGAHSDVIK VVKSKKLRAG KGKYRNRRFT QRRGPLVVYS EDNGVVKAFR NVPGVETANV ASLGLLQLAP GA HLGRFVI WTQSAFSKLD QIWGSETVAS VKAGYALPSN IISTSDVTRI INSTEIQSVL RAAGQATQKR THVQKKNPLK NKQ VLLRLN PYAKVFAAEK LGSKKVDQAN KIKPSATALE TLKH

UniProtKB: KLLA0B07139p

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Macromolecule #7: KLLA0D06941p

MacromoleculeName: KLLA0D06941p / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 33.734805 KDa
SequenceString: FIKDIKTSAY HSRYQTPFRR RREGKTDYYA RKRLVAQHKA KYNTPKYRLV VRFTNKDIIT QIVSSTITGD IVLAAAYSHE LPRYGITHG LTNWSAAYAT GLLLARRTLQ KLGLDETYPG VEEVEGEYEL TEAVEDGPRP FKVYLDVGLQ RTTTGARIFG A LKGASDGG ...String:
FIKDIKTSAY HSRYQTPFRR RREGKTDYYA RKRLVAQHKA KYNTPKYRLV VRFTNKDIIT QIVSSTITGD IVLAAAYSHE LPRYGITHG LTNWSAAYAT GLLLARRTLQ KLGLDETYPG VEEVEGEYEL TEAVEDGPRP FKVYLDVGLQ RTTTGARIFG A LKGASDGG LYVPHSENRF PGWDFEAEEL DADLLRTYIF GGHVSQYMEE LADDDEERYR ELFKGYLADD IDADAVEDIY AD AHAAIRE DPAFKPTEKK FTKEQYAAES KKYRQVKLTR EERKARVAAK ISALAPQE

UniProtKB: KLLA0D06941p

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Macromolecule #8: KLLA0B04686p

MacromoleculeName: KLLA0B04686p / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 19.49274 KDa
SequenceString:
KWYPAEEVPA PKQTRKAARP QKLRASLVPG TVLILLAGRF RGKRVVYLKH LEDNTLLVSG PFKVNGVPLR RVNARYVIAT STRVSLEGV NTEKFNVAYF SREKLSRKQR AEANFFNEDQ PKKEIKAERI EDQKNIDKAL LAEIKKTPLL KQYLAATFSL K NGDRPHLL KF

UniProtKB: KLLA0B04686p

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Macromolecule #9: KLLA0D03410p

MacromoleculeName: KLLA0D03410p / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 25.111957 KDa
SequenceString: QKTAEQVAAE RVARKAEKRA AILERNAAYQ KEYETAERAV IDAKREAKAN SSYYVSAEAK LVFVVRIKGI NKIPPKPRKV LQLLRLNQI NSGTFVKVTK ATSELLKLIE PYVAYGYPSY STVRQLVYKR GYGKINKQRI PLSDNSIVEA NLGNYGILSV D DLIHEIVT ...String:
QKTAEQVAAE RVARKAEKRA AILERNAAYQ KEYETAERAV IDAKREAKAN SSYYVSAEAK LVFVVRIKGI NKIPPKPRKV LQLLRLNQI NSGTFVKVTK ATSELLKLIE PYVAYGYPSY STVRQLVYKR GYGKINKQRI PLSDNSIVEA NLGNYGILSV D DLIHEIVT VGPHFKQANN FLWPFKLSNP SGGWGVPRKF KHFIQGGSFG NREEFINKLV KSMN

UniProtKB: KLLA0D03410p

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Macromolecule #10: KLLA0E00573p

MacromoleculeName: KLLA0E00573p / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 25.954338 KDa
SequenceString: NPLTKSSPRN FGIGQAIQPK RNLSRYVKWP EYVRLQRQKK ILSIRLKVPP SIAQFQNTLD RNTAAETFKL LKKYSPETAA EKKERLTKE AAAIAEGKTK QEASPKPYVV KYGLNHVVSL IENKKAKLVL IANDVDPIEL VIFLPALCKK MGVPYAIVKG K ARLGTLVN ...String:
NPLTKSSPRN FGIGQAIQPK RNLSRYVKWP EYVRLQRQKK ILSIRLKVPP SIAQFQNTLD RNTAAETFKL LKKYSPETAA EKKERLTKE AAAIAEGKTK QEASPKPYVV KYGLNHVVSL IENKKAKLVL IANDVDPIEL VIFLPALCKK MGVPYAIVKG K ARLGTLVN QKTSAVAALT EVREEDEAAL AKLVSTINAN YLEKYEDSKK HWGGGIMGQK AQKKIEKRAK AAESA

UniProtKB: 60S ribosomal protein L8

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Macromolecule #11: KLLA0F04499p

MacromoleculeName: KLLA0F04499p / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 21.730055 KDa
SequenceString:
MKYIQTDQVL DVPEGVTVQI RSRIIKVTGP RGVLTKNLKH IDVTFTKVSN RQIKITVHNG DRKHVAALRT VKSLISNLIT GVTKGYKYK LRYVYAHFPI NVNVIEKDGA KFIEIRNFLG DKKVREVPVR EGVSVDFSTN QKDEIVLSGN SVENVSQNAA D IQQICRVR NKDIRKFLDG IYVSEKGLIE EEE

UniProtKB: KLLA0F04499p

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Macromolecule #12: KLLA0D05643p

MacromoleculeName: KLLA0D05643p / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 24.997957 KDa
SequenceString: RRPARCYRYQ KNKPYPKSRY NRAVPDSKIR IYDLGKKKAT VDEFPLCVHL VSNELEQLSS EALEAARITA NKYITKMTGR DSFHLRVRV HPFHVLRINK MLSCAGADRL QQGMRGAWGK PHGLAARVAI GQVIFSVRTK DNNKDTVIEG LRRARYKFPG Q QKIIISKK ...String:
RRPARCYRYQ KNKPYPKSRY NRAVPDSKIR IYDLGKKKAT VDEFPLCVHL VSNELEQLSS EALEAARITA NKYITKMTGR DSFHLRVRV HPFHVLRINK MLSCAGADRL QQGMRGAWGK PHGLAARVAI GQVIFSVRTK DNNKDTVIEG LRRARYKFPG Q QKIIISKK WGFTSLNREE YVKKRDAGEI KDDGAFVKFL SKKGSLEENI REFPEYFA

UniProtKB: KLLA0D05643p

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Macromolecule #13: KLLA0F08261p

MacromoleculeName: KLLA0F08261p / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 19.203053 KDa
SequenceString:
QNPMRELKIE KLVLNISVGE SGDRLTRASK VLEQLSGQTP VQSKARYTVR TFGIRRNEKI AVHVTVRGPK AEEILERGLK VKEYQLRNR NFSATGNFGF GIQEHIDLGI KYDPGIGIYG MDFYIVMGRP GARVTRRKRC QSKIGNSHKT SKEDTIAWFK Q RYDAEVLD

UniProtKB: KLLA0F08261p

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Macromolecule #14: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 22.441969 KDa
SequenceString: AISKNLPILK NHFRKHWQER VKVHFDQAGK KVSRRHARAS KAAKIAPRPL DLLRPVVRAP TVKYNRKVRA GRGFTLAEIK AAGLTPAYA RTIGIAVDHR RQNRNQEILD LNIQRLKEYQ SKIIVFPRKG KAPEAEQTLS AAASFPIVQP VTDSETRAVE D NGESAYRT ...String:
AISKNLPILK NHFRKHWQER VKVHFDQAGK KVSRRHARAS KAAKIAPRPL DLLRPVVRAP TVKYNRKVRA GRGFTLAEIK AAGLTPAYA RTIGIAVDHR RQNRNQEILD LNIQRLKEYQ SKIIVFPRKG KAPEAEQTLS AAASFPIVQP VTDSETRAVE D NGESAYRT LRLARSDKRY KGIREKRAKD KAEAEAEKK

UniProtKB: 60S ribosomal protein L13

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Macromolecule #15: KLLA0B13409p

MacromoleculeName: KLLA0B13409p / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 14.835362 KDa
SequenceString:
STDSVVKASN WRLVEVGRVV LVKQGQSAGK LATIVEIIDQ KRALIDGPTT GVPRQAINLG QVVLTPLTFA VPRGAKSATV SKKWAAAGV SEKWATSSWA KKIAQRERRA ALSDFERFQV LVLKKQKRYA VKKAVAK

UniProtKB: KLLA0B13409p

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Macromolecule #16: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 24.19393 KDa
SequenceString: GAYKYLEELQ RKKQSDVLRF LQRVRVWEYR QKNVIHRASR PTRPDKARRL GYKAKQGFVI YRVRVRRGNR KRPVSKGATY GKPTNQGVN QLKYQRSLRA TAEERVGRRA ANLRVLNSYW INQDSTYKYF EVILVDPSHK AIRRDAKYNW ICNPVHKHRE A RGLTATGK ...String:
GAYKYLEELQ RKKQSDVLRF LQRVRVWEYR QKNVIHRASR PTRPDKARRL GYKAKQGFVI YRVRVRRGNR KRPVSKGATY GKPTNQGVN QLKYQRSLRA TAEERVGRRA ANLRVLNSYW INQDSTYKYF EVILVDPSHK AIRRDAKYNW ICNPVHKHRE A RGLTATGK KSRGINKGHK FHNTQAGRRK TWKRQNTLSL WRYR

UniProtKB: Ribosomal protein L15

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Macromolecule #17: KLLA0F04675p

MacromoleculeName: KLLA0F04675p / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 22.254148 KDa
SequenceString: SSFEPVVVID GKGHLLGRLA STVAKQLLNG QKIVVVRAEA LNISGEFFRN KLKYHDYLRK ATAFNKTRGP FHFRAPSRIF YKAVRGMIS HKTARGKAAL ERLKVFEGVP PPYDKKKRVV VPQALRVLRL KPGRKYTTLG KLSTSVGWKY EDVVAKLEEK R KARSAEYY ...String:
SSFEPVVVID GKGHLLGRLA STVAKQLLNG QKIVVVRAEA LNISGEFFRN KLKYHDYLRK ATAFNKTRGP FHFRAPSRIF YKAVRGMIS HKTARGKAAL ERLKVFEGVP PPYDKKKRVV VPQALRVLRL KPGRKYTTLG KLSTSVGWKY EDVVAKLEEK R KARSAEYY LKKKAYNAVV ANKSATVGTE VSEKLAALGY

UniProtKB: KLLA0F04675p

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Macromolecule #18: KLLA0A06336p

MacromoleculeName: KLLA0A06336p / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 20.322045 KDa
SequenceString:
RYGATSTNPA KSASARGSYL RVSYKNTRET AQAISGWNLQ KAQKYLDQVL DHQRAIPFRR FNSSIGRTAQ GKEFGVTKAR WPAKSVKFV QGLLQNAASN AEAKGLDATR LYVSHIQVNQ APKQRRRTFR AHGRINKYES SPSHIELVVT EKEEAVEKAS E KKVARLSS RQRGRLATQK RI

UniProtKB: KLLA0A06336p

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Macromolecule #19: KLLA0A07227p

MacromoleculeName: KLLA0A07227p / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 20.479945 KDa
SequenceString:
IDHTSKQHKR SSHRTAPKSD NVYLKLLVKL YAFLARRTDA PFNKVILKSL FLSKINRPPV SVSRISRALK QEGAAQKTIV VVGTVTDDN RIFEFPKTTV AALRFTAGAK AKILKAGGEA ITLDQLAVRA PKGQNTLIVR GPRASREAAR HFGFGPHKGK A PRIQSTGR KFERARGRRR SRAFKV

UniProtKB: KLLA0A07227p

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Macromolecule #20: KLLA0E12453p

MacromoleculeName: KLLA0E12453p / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 21.688285 KDa
SequenceString:
ANLRTQKRLA ASVAGVGKRK IWMDPSETAE IAQANSRNAI RKLVKNGTIV KKPTRIHSRS RTRAMAESKR NGRHTGYGKR KGTKEARLP SQVVWIRRLR VLRRLLAKYR DAGKIDRHLY HSLYKSAKGN TFKHKRALVE HIIQAKADAQ REKALKEEAE A RRSKNRAA RERRAQRVAE KREALLKEDA

UniProtKB: KLLA0E12453p

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Macromolecule #21: 60S ribosomal protein L20

MacromoleculeName: 60S ribosomal protein L20 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 20.046396 KDa
SequenceString:
FKEYQVIGRR LPTESVPEPK LFRMRIFAPN DVVAKSRYWY FLQKLHKVKK ASGEIVSLNV ISEAHPTTVK NFGVWVRYDS RSGTHNMYK EIRDVTRVGA VESLYQDMAA RHRARFRSIH ILKVVELEKT ADVKRQYVKQ FLTKDLKFPL PHRVQKSTKV F AYKRPTTF Y

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Macromolecule #22: KLLA0E23651p

MacromoleculeName: KLLA0E23651p / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 17.969895 KDa
SequenceString:
GKSHGYRSRT RYMFQRDFRK HGAIALSTYM KVYKVGDIVD IKANGSIHKG MPHKFYQGKT GVVYNVTKSS VGIIINKMVG NRYLEKRLN LRVEHIKHSK CRQEFLERVK SNAAKKAEAK AQGVAVQLKR QPAQPKESRV VSTEGNVPQT LAPVPYETF

UniProtKB: KLLA0E23651p

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Macromolecule #23: KLLA0D05181p

MacromoleculeName: KLLA0D05181p / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 11.419034 KDa
SequenceString:
QKITKTFTVD VSSPTENGVF DPASYAKYLI DHIKVEGHVG NLGQAITVEE DGSVVTIVST TKFSGKYLKY LTKKYLKKNQ LRDWIRFVS TKTNEYKLAF Y

UniProtKB: KLLA0D05181p

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Macromolecule #24: KLLA0E06997p

MacromoleculeName: KLLA0E06997p / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 13.997389 KDa
SequenceString:
GAQGTKFRIS LGLPTGAIMN CADNSGARNL YIMAVKGSGS RLNRLPAASL GDMVMATVKK GKPELRKKVM PAIVVRQSKA WRRKDGVFL YFEDNAGVIA NPKGEMKGSA VTGPVGKECA DLWPRIASNS GVV

UniProtKB: KLLA0E06997p

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Macromolecule #25: 60S ribosomal protein L24

MacromoleculeName: 60S ribosomal protein L24 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 7.279468 KDa
SequenceString:
KVEIDSFSGA KIYPGRGTLF VRGDSKIFRF QSSKSASLFH QRKNPRRIAW TVLYRRHHKK GI

UniProtKB: Large ribosomal subunit protein eL24

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Macromolecule #26: 60S ribosomal protein L25

MacromoleculeName: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 13.700033 KDa
SequenceString:
KALKVRTSAS FRLPKTLKLA RSPKYATKAV PHYNRLDSYK VIEQPITSET AMKKVEDGNT LVFKVSLKAN KYQIKKAVKE LYEVDVLSV NTLVRPNGTK KAYVRLTADF DALDIANRIG YI

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #27: KLLA0B05742p

MacromoleculeName: KLLA0B05742p / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 14.127461 KDa
SequenceString:
KQSLDVSSDR RKARKAYFTA PSSERRVLLS APLSKELREQ YNIKALPIRK EDEVLVVRGS KKGQEGKVSS VYRLKFAVQV DKLTKEKSN GASVPTNIHP SKVVITKLHL DKDRKALIQR KGGKLE

UniProtKB: KLLA0B05742p

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Macromolecule #28: KLLA0E03455p

MacromoleculeName: KLLA0E03455p / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 15.398082 KDa
SequenceString:
KFLKAGKVAV VVRGRYAGKK VVIVKPHDEG TKSHPFGHAL VAGIERYPLK VTKRQGAKKV AKRTKIKPFI KVINYNHLLP TRYTLDVEA FKSVVSTETF EEPSQREEAK KVIKKAFEER HQAGKNQWFF TKLNF

UniProtKB: KLLA0E03455p

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Macromolecule #29: RPL28

MacromoleculeName: RPL28 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 16.382205 KDa
SequenceString:
PTRLTKTRKH RGHVSAGKGR VGKHRKHPGG RGMAGGLHHH RTNLDKYHPG YFGKVGMRYF HKQQAHFWKP VLNLDKLWTL VPEEKREEY LKSASKSAAP VIDTLAAGYG KVLGKGRIPD VPVIVKARFV SKLAEEKIKA AGGVVELI

UniProtKB: RPL28

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Macromolecule #30: KLLA0D16071p

MacromoleculeName: KLLA0D16071p / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 6.500615 KDa
SequenceString:
KSKNHTAHNQ TKKAHRNGIK KPKTYKYPSL KGVDAKFRRN HRFALHGTAK ALAAKRA

UniProtKB: 60S ribosomal protein L29

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Macromolecule #31: 60S ribosomal protein L30

MacromoleculeName: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 10.511289 KDa
SequenceString:
NINQKLALVV KSGKYSLGYK STVKSLRQGK AKLIIIAANT PVLRKSELEY YAMLSKTKVY YFQGGNNELG TAVGKLFRVG VVTILDAGD SDILTALA

UniProtKB: Large ribosomal subunit protein eL30

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Macromolecule #32: KLLA0B02937p

MacromoleculeName: KLLA0B02937p / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 12.360539 KDa
SequenceString:
KDVVTREYTI NMHKRLHGVS FKKRAPKAVK EIKKFAKLHM GTDDVRLDPK LNQEIWKRGI KGVPFRLRLR ISRRRNEEED AKNPLFSYV EPVVVASAKG LHTVVVD

UniProtKB: KLLA0B02937p

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Macromolecule #33: KLLA0E06843p

MacromoleculeName: KLLA0E06843p / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 13.941428 KDa
SequenceString:
LHPKIVKKHT KKFKRHHSDR YHRVSENWRK QKGIDSVVRR RFRGNISEPT IGYGSNKKTK FMGPDGRKVV LVSNVKGLTP LLMHTKTFA AEIAHNVSSK NRIAILAEAK KNGIKVTNAK ARL

UniProtKB: KLLA0E06843p

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Macromolecule #34: KLLA0D07405p

MacromoleculeName: KLLA0D07405p / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 11.863668 KDa
SequenceString:
AESHRLYVKG KHLSYQRSKS VNNPNVSLVK IEGVANPEDA KFYLGKRVAY VYRCSKEVRG SKIRVIWGKI NRTHGNSGVV RATFRSNLP AKTFGASVRI FLYPSN

UniProtKB: KLLA0D07405p

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Macromolecule #35: KLLA0C08371p

MacromoleculeName: KLLA0C08371p / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 13.579934 KDa
SequenceString:
AQRVTFRRRN PYNTKSNKIK VVKTPGGALR SQHVKKLATR PKCGDTGVPL QGVSTLRPRQ YATVSRTKKT VSRAYGGSKS ANAVKERIV RAFLIEEQKI VKRVIKEQTE AAKKAEKKDA KK

UniProtKB: KLLA0C08371p

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Macromolecule #36: KLLA0F05247p

MacromoleculeName: KLLA0F05247p / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 13.677186 KDa
SequenceString:
GIKAYELRTK SKDQLEQQLV ELKKELAELK VQKLSRPSLP KINTVRKNIA RVLTVISQNQ RQAVRELYKG KKYQPKDLRA KKTRALRRA LTKFEASQVT EKQRKKQIAF PQRKYAI

UniProtKB: KLLA0F05247p

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Macromolecule #37: 60S ribosomal protein L36

MacromoleculeName: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 10.925905 KDa
SequenceString:
VKSGIAVGLN KGKKVNQLTP APKISYRKGA ASQRTTFVRS IVKEVASLAP YERRLIELIR NAGEKRARKV AKKRLGTFGR AKAKVEEMN EIITASRRH

UniProtKB: 60S ribosomal protein L36

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Macromolecule #38: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 9.679198 KDa
SequenceString:
GKGTPSFGKR HNKSHTLCKR CGRSSYHIQK HTCSSCGYPA AKMRSHNWAA KAKRRRTTGT GRMRYLKHVS RRFKNGFQSG EAKAQ

UniProtKB: Ribosomal protein L37

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Macromolecule #39: KLLA0C18216p

MacromoleculeName: KLLA0C18216p / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 8.794387 KDa
SequenceString:
KEIADIKEFL ELIRRQDVNS ATVKINKKLN KNGKAFRQTK FKLRGSRYLY TLIVNDAGKA KKLLQSLPPT FNVNKL

UniProtKB: KLLA0C18216p

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Macromolecule #40: 60S ribosomal protein L39

MacromoleculeName: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 6.114286 KDa
SequenceString:
AAQKSFRIKQ KMAKAKKQNR PLPQWIRLRT NNTIRYNAKR RNWRRTKMN

UniProtKB: Large ribosomal subunit protein eL39

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Macromolecule #41: Ubiquitin fusion protein

MacromoleculeName: Ubiquitin fusion protein / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 5.933189 KDa
SequenceString:
IEPSLKALAS KYNCEKSVCR KCYARLPPRA TNCRKRKCGH TNQLRPKKKL K

UniProtKB: Ubiquitin fusion protein

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Macromolecule #42: 60S ribosomal protein L41-A

MacromoleculeName: 60S ribosomal protein L41-A / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 3.354243 KDa
SequenceString:
MRAKWRKKRT RRLKRKRRKV RARSK

UniProtKB: Small ribosomal subunit protein eS32A

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Macromolecule #43: 60S ribosomal protein L44

MacromoleculeName: 60S ribosomal protein L44 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 11.676897 KDa
SequenceString:
VNVPKTRKTY CKGKECRKHA QHKVTQYKAG KASLYAQGKR RYDRKQSGFG GQTKQIFHKK AKTTKKVVLR LECMSCKTKT QLALKRCKH FELGGEKKQK GQ

UniProtKB: Large ribosomal subunit protein eL42

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Macromolecule #44: KLLA0E05941p

MacromoleculeName: KLLA0E05941p / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 9.497062 KDa
SequenceString:
KRTKKVGITG KYGVRYGSSL RRQVKKLEVQ QHATYNCSFC GKTCVKRGAA GVWSCSSCNR TIAGGAYTLS TAAAATVRST IRRLRDM

UniProtKB: KLLA0E05941p

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Macromolecule #45: Ribosomal protein

MacromoleculeName: Ribosomal protein / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 24.554867 KDa
SequenceString: MSKITSSHVR EHVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS GTLKLPVCPR PNLSICIFGD AFDVDRAKSC GVDAMSVED LKKLNKNKKL IKKLAKKYSA FVASEVLIKQ VPRLLGPQLS KAGKFPTPVS HNDDLYQKVN DVRSTIKFQL K KVLCLAVA ...String:
MSKITSSHVR EHVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS GTLKLPVCPR PNLSICIFGD AFDVDRAKSC GVDAMSVED LKKLNKNKKL IKKLAKKYSA FVASEVLIKQ VPRLLGPQLS KAGKFPTPVS HNDDLYQKVN DVRSTIKFQL K KVLCLAVA VGNVEMDEDT LVNQILMSVN FLVSLLKKNW QNVGSLVIKS TMGPAYRLY

UniProtKB: Ribosomal protein

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Macromolecule #46: 60S acidic ribosomal protein P0

MacromoleculeName: 60S acidic ribosomal protein P0 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 21.411648 KDa
SequenceString: EKKAEYFAKL REYLEEYKSV FVVGVDNVSS QQMHEVRKNL RGRAVVLMGK NTMVRRAVRG FISDFPDYEK LLPFVKGNVG FIFTNDSLQ DIKEVIIANK VAAPARAGAV APEDIWVLAV NTGMEPGKTS FFQALGVPTK IARGTIEIVS DVKVVDAGKR V GASEASLL ...String:
EKKAEYFAKL REYLEEYKSV FVVGVDNVSS QQMHEVRKNL RGRAVVLMGK NTMVRRAVRG FISDFPDYEK LLPFVKGNVG FIFTNDSLQ DIKEVIIANK VAAPARAGAV APEDIWVLAV NTGMEPGKTS FFQALGVPTK IARGTIEIVS DVKVVDAGKR V GASEASLL NLLNISPFTY GLTVVQVYDN GQVFPAS

UniProtKB: 60S acidic ribosomal protein P0

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Macromolecule #47: uL11

MacromoleculeName: uL11 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 12.528436 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)

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Macromolecule #48: 40S ribosomal protein S0

MacromoleculeName: 40S ribosomal protein S0 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 22.882172 KDa
SequenceString: SLPSTFDLTS EDAQLLLAAR VHLGAKNVQV HQEPYVYKAR PDGVNVINVG KTWEKIVLAA RIIAAIPNPE DVVAISSRTY GQRAVLKYA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL VIVTDPRSDA QAIKESSYVN IPVIALTDLD SPSEYVDVAI P CNNRGKHS ...String:
SLPSTFDLTS EDAQLLLAAR VHLGAKNVQV HQEPYVYKAR PDGVNVINVG KTWEKIVLAA RIIAAIPNPE DVVAISSRTY GQRAVLKYA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL VIVTDPRSDA QAIKESSYVN IPVIALTDLD SPSEYVDVAI P CNNRGKHS IGLIWYLLAR EVLRLRGALP DRTQPWAIMP DLYFYRNP

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #49: 40S ribosomal protein S1

MacromoleculeName: 40S ribosomal protein S1 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 24.488287 KDa
SequenceString: VVDPFTRKEW YDIKAPSTFE NRNVGKTLVN KSVGLKNASD SLKGRVVEVC LADLQGSEDH SFRKVKLRVD EVQGKNLLTN FHGMDFTTD KLRSMVRKWQ TLIEANVTVK TSDDYVLRIF AIAFTRKQAN QVKRTSYAQS SHIRQIRKVI SEILTREVQN S TLAQLTSK ...String:
VVDPFTRKEW YDIKAPSTFE NRNVGKTLVN KSVGLKNASD SLKGRVVEVC LADLQGSEDH SFRKVKLRVD EVQGKNLLTN FHGMDFTTD KLRSMVRKWQ TLIEANVTVK TSDDYVLRIF AIAFTRKQAN QVKRTSYAQS SHIRQIRKVI SEILTREVQN S TLAQLTSK LIPEVINKEI ENATKDIFPL QNVHIRKVKL LKQPKFDLGS LLSLHG

UniProtKB: Small ribosomal subunit protein eS1

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Macromolecule #50: KLLA0F09812p

MacromoleculeName: KLLA0F09812p / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 23.162922 KDa
SequenceString: GWVPVTKLGR LVKAGKISSI EEIFLHSLPV KEFQIIDQLL PNLKDEVMNI KPVQKQTRAG QRTRFKAVVV VGDSNGHVGL GIKTAKEVA GAIRAGIIIA KLSVIPIRRG YWGTNLGQPH SLATKTSGKC GSVSVRLIPA PRGSGIVASP AVKKLMQLAG V EDVYTSST ...String:
GWVPVTKLGR LVKAGKISSI EEIFLHSLPV KEFQIIDQLL PNLKDEVMNI KPVQKQTRAG QRTRFKAVVV VGDSNGHVGL GIKTAKEVA GAIRAGIIIA KLSVIPIRRG YWGTNLGQPH SLATKTSGKC GSVSVRLIPA PRGSGIVASP AVKKLMQLAG V EDVYTSST GSTRTLENTL KAAFVAIGNT YGFLTPNLWE VQALTPSPMD VYADYATAS

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #51: KLLA0D08305p

MacromoleculeName: KLLA0D08305p / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 24.830895 KDa
SequenceString: AIISKKRKLV ADGVFYAELN EFFTRELAEE GYSGVEVRVT PTKTEIIIRA TKVQDVVGEN GRRINELTLL IEKRFKYKRG TIALYAERV HDRGLSAVAQ AESMKFKLLN GLAIRRAAYG VVRYVMESGA KGCEVVISGK LRAARAKSMK FADGFLIHSG Q PVNDFIET ...String:
AIISKKRKLV ADGVFYAELN EFFTRELAEE GYSGVEVRVT PTKTEIIIRA TKVQDVVGEN GRRINELTLL IEKRFKYKRG TIALYAERV HDRGLSAVAQ AESMKFKLLN GLAIRRAAYG VVRYVMESGA KGCEVVISGK LRAARAKSMK FADGFLIHSG Q PVNDFIET ATRHVLLRQG VLGIKVKIMK DPSRNTSGPK ALPDAVTIIE PKEEEPVLEP SVKDY

UniProtKB: 40S ribosomal protein S3

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Macromolecule #52: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 29.486312 KDa
SequenceString: ARGPKKHLKR LAAPHHWMLD KLSGCYAPRP SAGPHKLRES LPLIVFLRNR LKYALNGREV KAILMQRHVK VDGKVRTDTT FPAGFMDVI TLEATNENFR LVYDVKGRFA VHRITDEEAS YKLAKVKKVQ LGKKGIPYVV THDGRTIRYP DPNIKVNDTV K VDLATGTI ...String:
ARGPKKHLKR LAAPHHWMLD KLSGCYAPRP SAGPHKLRES LPLIVFLRNR LKYALNGREV KAILMQRHVK VDGKVRTDTT FPAGFMDVI TLEATNENFR LVYDVKGRFA VHRITDEEAS YKLAKVKKVQ LGKKGIPYVV THDGRTIRYP DPNIKVNDTV K VDLATGTI TDFIKFDTGK LVYVTGGRNL GRVGTIVHRE RHEGGFDLVH IKDSLENTFV TRLNNVFVIG EPGRPWISLP KG KGIKLTI SEERDRRRAQ HGL

UniProtKB: 40S ribosomal protein S4

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Macromolecule #53: KLLA0D10659p

MacromoleculeName: KLLA0D10659p / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 22.912369 KDa
SequenceString: FVPVELATTI PVEIQQAQQE IKLFNKWSFE DVEVKDASLV DYIQISKPIY VAHTAGRYAN KRFRKAQCPI VERLTNSLMM NGRNNGKKL KAVRIVKHTL EIINVLTDQN PLQVVVDAII NSGPREDTTR VGGGGAARRQ AVDVSPLRRV NQSIALLTIG A REAAFRNI ...String:
FVPVELATTI PVEIQQAQQE IKLFNKWSFE DVEVKDASLV DYIQISKPIY VAHTAGRYAN KRFRKAQCPI VERLTNSLMM NGRNNGKKL KAVRIVKHTL EIINVLTDQN PLQVVVDAII NSGPREDTTR VGGGGAARRQ AVDVSPLRRV NQSIALLTIG A REAAFRNI KTIAETLAEE LINAAKGSST SYAIKKKDEL ERVAKSNR

UniProtKB: KLLA0D10659p

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Macromolecule #54: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 25.810977 KDa
SequenceString: MKLNISYPIN GTQKCIEIDD EHRVRVFYDK RIGQEVDGES VGDEFKGYVF KIAGGNDKQG FPMKQGVLLP TRVKLLLAKG HSCYRPRRN GERKRKSVRG AIVGPDLAVL ALIITKKGEQ EIEGITNDTV PKRLGPKRAN NIRKFFGLTK EDDVRDYVIR R EVTKGDKS ...String:
MKLNISYPIN GTQKCIEIDD EHRVRVFYDK RIGQEVDGES VGDEFKGYVF KIAGGNDKQG FPMKQGVLLP TRVKLLLAKG HSCYRPRRN GERKRKSVRG AIVGPDLAVL ALIITKKGEQ EIEGITNDTV PKRLGPKRAN NIRKFFGLTK EDDVRDYVIR R EVTKGDKS YTKAPKIQRL VTPQRLQRKR QQKSLKIKNA QAQREAAAEY AQLLAKRLSE RKAEKAEV

UniProtKB: Small ribosomal subunit protein eS6

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Macromolecule #55: KLLA0C13519p

MacromoleculeName: KLLA0C13519p / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 21.03357 KDa
SequenceString:
PQAKILSQAP TELELQVAQA FIDLENNSPE LKADLRALQF KSIREIEVAG GKKALAVFVP VPSLAAYHKV QIKLTRELEK KFQDRHVIF LAERRILPKP SRKSRQTQKR PRSRTLTAVH DKILEDLVFP TEIVGKRVRY LVGGNKIQKI LLNSKDVQHI D NKLESFQA VYNKLTGKQI VFEIPS

UniProtKB: 40S ribosomal protein S7

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Macromolecule #56: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 22.511531 KDa
SequenceString: GISRDSRHKR AATGAKRAQF RKKRKFELGR QAANTKIGTK RIHPVRTRGG NQKFRALRIE TGNFSWASEG VARKTRITGV VYHPSNNEL VRTNTLTKAA IVQIDATPFR QWYESHYGQS LGKKKNTKAE EETATTSKNT ERKWAARAAE AKIEHAVDSQ F GAGRLYAA ...String:
GISRDSRHKR AATGAKRAQF RKKRKFELGR QAANTKIGTK RIHPVRTRGG NQKFRALRIE TGNFSWASEG VARKTRITGV VYHPSNNEL VRTNTLTKAA IVQIDATPFR QWYESHYGQS LGKKKNTKAE EETATTSKNT ERKWAARAAE AKIEHAVDSQ F GAGRLYAA ISSRPGQSGR CDGYILEGEE LAFYLRRLTA KK

UniProtKB: 40S ribosomal protein S8

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Macromolecule #57: KLLA0E23673p

MacromoleculeName: KLLA0E23673p / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 20.882344 KDa
SequenceString:
PRAPRTYSKT YSTPKRPYES ARLDAELKLA GEYGLKNKRE IYRISFQLSK IRRAARDLLT RDEKDPKRLF EGNALIRRLV RIGVLSEDK KKLDYVLALK VEDFLERRLQ TQVYKLGLAK SVHHARVLIS QRHIAVGKQI VNIPSFMVRL ESEKHIDFAR T SPFGGARP GRVARKRAAA AGGE

UniProtKB: KLLA0E23673p

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Macromolecule #58: KLLA0B08173p

MacromoleculeName: KLLA0B08173p / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 11.423085 KDa
SequenceString:
MLIPKEDRKK IYQHLFQEGV LVAKKDFNQP KHEEIDTKNL FVIKALQSLT SKGFVKTQFS WQYYYYTLTE EGVVYLREYL NLPEHIFPA TYLAGQS

UniProtKB: KLLA0B08173p

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Macromolecule #59: KLLA0A10483p

MacromoleculeName: KLLA0A10483p / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 17.712734 KDa
SequenceString:
STELTVQSER AFQKQPHIFT NPKAKANRKT KRWYKNVGLG FKTPKTAIEG SYIDKKCPFT GLVSIRGKIL TGTVVSTRMH RTIVIRRDY LHYVPKYNRY EKRHKNVPAH VSPAFRVQVG DIVTVGQCRP ISKTVRFNVL KVASATGKAN KQFAKF

UniProtKB: KLLA0A10483p

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Macromolecule #60: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 13.124912 KDa
SequenceString:
AELTIEDALK VVLRTSLVHD GLARGLRESA KALTRGEGQL AVLVESVTEE AISKLVQGLA TENNVPLIKV ADAKQLGEWA GLGKIDRDG NARKVVGASV VVVKNWGADT QEREILLEHF SQQ

UniProtKB: 40S ribosomal protein S12

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Macromolecule #61: KLLA0F18040p

MacromoleculeName: KLLA0F18040p / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 16.858678 KDa
SequenceString:
GRMHSKGKGM SSSAIPYSRN APAWFKGSSD GVVEQIIKYA RKGLTPSQIG VLLRDAHGVT QAKVITGNKI LRILKSNGLA PEIPEDLYF LIKKAVSVRK HLERNRKDKD AKFRLILIES RIHRLARYYR TVSVLPPNWK YESATASALV N

UniProtKB: KLLA0F18040p

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Macromolecule #62: 40S ribosomal protein S14

MacromoleculeName: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 13.458439 KDa
SequenceString:
SQVFGVARIF ASFNDTFVHV TDLSGRETIA RVTGGMKVKA DRDESSPYAA MLAAQDVAAK CKEVGITAVH IKIRATGGTR SKTPGPGGQ AALRALARSG LRIGRIEDVT PVPSDSTRKK GGRRGRRL

UniProtKB: Small ribosomal subunit protein uS11

+
Macromolecule #63: KLLA0F07843p

MacromoleculeName: KLLA0F07843p / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 13.969457 KDa
SequenceString:
RKRSFKTYSY KGVDLEKLLE MPTEDFVKLA PARVRRKFAR GLSEKPAGLM KKLRAAKLSA PENEKPAVVR THLRNMIIVP EMIGSVVGV YNGKVFNQVE IRPEMVGHYL GEFSITYTPV RHGA

UniProtKB: KLLA0F07843p

+
Macromolecule #64: 40S ribosomal protein S16

MacromoleculeName: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 15.656257 KDa
SequenceString:
TVPSVQTFGK KKSATAVAHV KAGKGLIKVN GSPITLVQPE ILRFKVYEPL LLVGLDKFAN IDIRVKVTGG GHVSQVYAIR QAIAKGLVA YHQKFVDEQS KNELKKAFTS YDRTLLIADS RRPEPKKFGG RGARSRFQKS YR

UniProtKB: Small ribosomal subunit protein uS9

+
Macromolecule #65: KLLA0B01474p

MacromoleculeName: KLLA0B01474p / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 14.72898 KDa
SequenceString:
GRVRTKTVKR ASKALIEKYY PKLTMDFQTN KRLCDEIATI QSKRLRNKIA GYTTHLMKRI QKGPVRGISF KLQEEERERK DQYVPDVSA LDLSHSNDVL NVDTQTAELV NSLGLKLPLS VSSVSAVRDR

UniProtKB: KLLA0B01474p

+
Macromolecule #66: KLLA0B01562p

MacromoleculeName: KLLA0B01562p / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 16.953404 KDa
SequenceString:
SLVVQEQGSF QHILRLLNTN VDGNINVVYA LTTIRGVGRR YANLVCKKAD VDLHKRAGEL TQEELERIVQ IMQNPTHYKI PAWFLNRQK DVNDGKDYHS LANNLESKLR DDLERLKKIR SHRGIRHFWG LRVRGQHTKT TGRRRA

UniProtKB: KLLA0B01562p

+
Macromolecule #67: KLLA0A07194p

MacromoleculeName: KLLA0A07194p / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 15.747814 KDa
SequenceString:
PGVSVRDVPA QDFINNYASF LQRQGKLEVP GYVDIVKTSA GNELPPQDSE GWFYKRAASV ARHIYLRKQV GVGKLNKLYG GAKNRGVRP HKHVDASGSI NRKVLQSLEK LGVVEISPKG GRRISDNGLR DLDRIAAATL EDEE

UniProtKB: KLLA0A07194p

+
Macromolecule #68: KLLA0F25542p

MacromoleculeName: KLLA0F25542p / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 12.032146 KDa
SequenceString:
QEVVIHKIRI NLTSTKVKQL ENVSANIIKN AETFKLVKKG PVRLPTKVLK ISTRKTPNGE GSKTWDTYEM RIHKRYIDLE APAHIVKRI TQITIEPGVD VEVIIAA

UniProtKB: KLLA0F25542p

+
Macromolecule #69: 40S ribosomal protein S21

MacromoleculeName: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 9.797949 KDa
SequenceString:
MENDKGQLVE LYVPRKCSAT NRIIKAKDHS SVQINIAQVD EEGRAIPGEY VTYALSGYIR ARGEADDSLN RLAQQDGLLK NVWSYSR

UniProtKB: Small ribosomal subunit protein eS21

+
Macromolecule #70: 40S ribosomal protein S22

MacromoleculeName: 40S ribosomal protein S22 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 14.513846 KDa
SequenceString:
TRTSVLADAL NAINNAEKTG KRQVLIRPSS KVIIKFLQVM QKHGYIGEFE YIDDHRSGKI VVQLNGRLNK CGVISPRFNV KIADVEKWT ANLLPARQFG YVILTTSAGI MDHEEAHRKH VSGKILGFVY

UniProtKB: Small ribosomal subunit protein uS8

+
Macromolecule #71: RPS23

MacromoleculeName: RPS23 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 16.047897 KDa
SequenceString:
MGKGKPRGLN SARKLRVHRR NNRWAETTYK KRLLGTAFKS SPFGGSSHAK GIVLEKIGIE SKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VSLLALWKEK KEKPRS

UniProtKB: RPS23

+
Macromolecule #72: 40S ribosomal protein S24

MacromoleculeName: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 15.063354 KDa
SequenceString:
SDAITIRTRK VISNPLLARK QFVVDVLHPN RANVSKDELR EKLAEAYKAE KDAVSVFGFR TQYGGGKSTG FGLVYNSVAD AKKFEPAYR LVRYGLAEKV EKASRQQRKQ RKNRGKKIFG TGKSIAKKAA RRNAD

UniProtKB: 40S ribosomal protein S24

+
Macromolecule #73: KLLA0B06182p

MacromoleculeName: KLLA0B06182p / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 7.939246 KDa
SequenceString:
AKHAVVLDQD KFDRIMKEAP TYRYVSVSVL VDRFKLGGSL ARVALRHLEN EGIIKPVSKH SKQAIYTRAT

UniProtKB: 40S ribosomal protein S25

+
Macromolecule #74: KLLA0D05115p

MacromoleculeName: KLLA0D05115p / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 11.454568 KDa
SequenceString:
MPKKRASNGR NKKGRGHVKP VRCVNCSRSV PKDKAIKRMA IRNIVEAAAI RDLSEASVYA EYALPKTYNK LHYCISCAIH ARIVRVRSR TDRRIRAPPQ R

UniProtKB: 40S ribosomal protein S26

+
Macromolecule #75: 40S ribosomal protein S27

MacromoleculeName: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 8.884362 KDa
SequenceString:
MVLVQDLLHP TAASEARKHK LKTLVQSPRS HFLDVKCPGC LNITTVFSHA QTAVTCESCS TVLCTPTGGK AKLSEGTSFR RK

UniProtKB: 40S ribosomal protein S27

+
Macromolecule #76: 40S ribosomal protein S28

MacromoleculeName: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 7.073256 KDa
SequenceString:
TPVTLAKVIK VLGRTGSRGG VTQVRVEFLE DTTRTIVRNV KGPVREGDIL VLMESEREAR RLR

UniProtKB: Small ribosomal subunit protein eS28

+
Macromolecule #77: 40S ribosomal protein S29

MacromoleculeName: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 6.322149 KDa
SequenceString:
ENVWYSHPRK FGKGSRQCRI SGSHSGLIRK YGLNIDRQSF REKANDIGFY KYR

UniProtKB: Small ribosomal subunit protein uS14

+
Macromolecule #78: KLLA0C04809p

MacromoleculeName: KLLA0C04809p / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 6.329429 KDa
SequenceString:
ARAGKVKSQT PKVEKQEKPK QPKGRAYKRL LYTRRFVNVT LTNGKRKMNP SPSSQ

UniProtKB: 40S ribosomal protein S30

+
Macromolecule #79: Ubiquitin-40S ribosomal protein S27a

MacromoleculeName: Ubiquitin-40S ribosomal protein S27a / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 7.958424 KDa
SequenceString:
KKVYTTPKKI RHKHKKVKLA VLNYYKVDDE GKVAKLRKEC PNCGPGIFLA NHGDRFYCGK CHSTFATQK

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

+
Macromolecule #80: KLLA0E12277p

MacromoleculeName: KLLA0E12277p / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 35.612676 KDa
SequenceString: SSNIMLVLRG TLEGHNGWVT SLSTSAAQPN LLVSGSRDKT LISWRLTENE QQFGVPVRSY KGHSHIVQDV VVSADGNYAV SASWDKTLR LWNLATGNSE ARFVGHTGDV LSVAIDANSS KIISASRDKT IRVWNTVGDC AYVLLGHTDW VTKVRVAPKN L EDGEVDDG ...String:
SSNIMLVLRG TLEGHNGWVT SLSTSAAQPN LLVSGSRDKT LISWRLTENE QQFGVPVRSY KGHSHIVQDV VVSADGNYAV SASWDKTLR LWNLATGNSE ARFVGHTGDV LSVAIDANSS KIISASRDKT IRVWNTVGDC AYVLLGHTDW VTKVRVAPKN L EDGEVDDG RITFVSAGMD KIVRSWSLNE DSYRIEADFI GHNNYINVVQ PSPDGSLAAS AGKDGQIYVW NLKHKSAFMN FD AKDEVFA LAFSPSRFWL TAATASGIKI YDLENEVLID ELKPEFAGYT KAQDPHAVSL AWSADGQTLF AGYTDNVIRV WQV MTAN

UniProtKB: KLLA0E12277p

+
Macromolecule #83: Eft2p

MacromoleculeName: Eft2p / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae P283 (yeast)
Molecular weightTheoretical: 91.592039 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: TVDQMRSLMD KVTNVRNMSV IAHVDHGKST LTDSLVQRAG IISAAKAGEA RFTDTRKDEQ ERGITIKSTA ISLYSEMSDE DVKEIKQKT DGNSFLINLI DSPGHVDFSS EVTAALRVTD GALVVVDTIE GVCVQTETVL RQALGERIKP VVVINKVDRA L LELQVSKE ...String:
TVDQMRSLMD KVTNVRNMSV IAHVDHGKST LTDSLVQRAG IISAAKAGEA RFTDTRKDEQ ERGITIKSTA ISLYSEMSDE DVKEIKQKT DGNSFLINLI DSPGHVDFSS EVTAALRVTD GALVVVDTIE GVCVQTETVL RQALGERIKP VVVINKVDRA L LELQVSKE DLYQTFARTV ESVNVIVSTY ADEVLGDVQV YPARGTVAFG SGLHGWAFTI RQFATRYAKK FGVDKAKMMD RL WGDSFFN PKTKKWTNKD TDAEGKPLER AFNMFILDPI FRLFTAIMNF KKDEIPVLLE KLEIVLKGEE KDLEGKALLK VVM RKFLPA ADALLEMIVL HLPSPVTAQA YRAEQLYEGP ADDANCIAIK NCDPKADLML YVSKMVPTSD KGRFYAFGRV FAGT VKSGQ KVRIQGPNYV PGKKDDLFIK AIQRVVLMMG RFVEPIDDCP AGNIIGLVGI DQFLLKTGTL TTSETAHNMK VMKFS VSPV VQVAVEVKNA NDLPKLVEGL KRLSKSDPCV LTYMSESGEH IVAGTGELHL EICLQDLEHD HAGVPLKISP PVVAYR ETV ESESSQTALS KSPNKHNRIY LKAEPIDEEV SLAIENGIIN PRDDFKARAR IMADDYGWDV TDARKIWCFG PDGNGPN LV IDQTKAVQYL HEIKDSVVAA FQWATKEGPI FGEEMRSVRV NILDVTLHAD AIHRGGGQII PTMRRATYAG FLLADPKI Q EPVFLVEIQC PEQAVGGIYS VLNKKRGQVV SEEQRPGTPL FTVKAYLPVN ESFGFTGELR QATGGQAFPQ MVFDHWSTL GSDPLDPTSK AGEIVLAARK RHGMKEE

UniProtKB: UNIPROTKB: W7R097

+
Macromolecule #84: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 84 / Number of copies: 80 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #85: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 85 / Number of copies: 6 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #86: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

MacromoleculeName: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / type: ligand / ID: 86 / Number of copies: 1 / Formula: GCP
Molecular weightTheoretical: 521.208 Da
Chemical component information

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

+
Macromolecule #87: (2S)-1-amino-N,N,N-trimethyl-1-oxobutan-2-aminium

MacromoleculeName: (2S)-1-amino-N,N,N-trimethyl-1-oxobutan-2-aminium / type: ligand / ID: 87 / Number of copies: 1 / Formula: 6EM
Molecular weightTheoretical: 145.223 Da
Chemical component information

ChemComp-6EM:
(2S)-1-amino-N,N,N-trimethyl-1-oxobutan-2-aminium

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 6.5 / Component - Concentration: 20.0 millimolar / Component - Name: MES-KOH
VitrificationCryogen name: ETHANE / Instrument: FEI VITROBOT MARK II

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 30.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Startup modelType of model: EMDB MAP
EMDB ID:

2421

Final reconstructionAlgorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 37844
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: RECIPROCAL / Protocol: AB INITIO MODEL
Output model

PDB-5it7:
Structure of the Kluyveromyces lactis 80S ribosome in complex with the cricket paralysis virus IRES and eEF2

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