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- EMDB-75921: Focused subtomogram average of the transition zone linker (linker... -

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Basic information

Entry
Database: EMDB / ID: EMD-75921
TitleFocused subtomogram average of the transition zone linker (linker2) in human airway cilia
Map data
Sample
  • Organelle or cellular component: Human airway ciliary transition zone (TZ) linker
KeywordsCilia / Transition Zone / TZ linker / Subtomogram Averaging / STRUCTURAL PROTEIN
Biological speciesHomo sapiens (human)
Methodsubtomogram averaging / cryo EM / Resolution: 7.0 Å
AuthorsZhou H / Brown A
Funding support United States, 3 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) United States
The Giovanni Armenise-Harvard Foundation United States
Richard and Susan Smith Family Foundation United States
CitationJournal: Science / Year: 2026
Title: In situ structure of the human ciliary transition zone links linker defects to primary ciliary dyskinesia
Authors: Zhou H / Brown A
History
DepositionMar 8, 2026-
Header (metadata) releaseSep 9, 2026-
Map releaseSep 9, 2026-
UpdateSep 9, 2026-
Current statusSep 9, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75921.png

Downloads & links

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Map

FileDownload / File: emd_75921.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.92 Å/pix.
x 480 pix.
= 923.52 Å		1.92 Å/pix.
x 480 pix.
= 923.52 Å		1.92 Å/pix.
x 480 pix.
= 923.52 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.924 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.002
Minimum - Maximum-0.009311105 - 0.024856986
Average (Standard dev.)0.00003872244 (±0.0005309209)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 923.52 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_75921_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_75921_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_75921_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Human airway ciliary transition zone (TZ) linker

EntireName: Human airway ciliary transition zone (TZ) linker
Components
  • Organelle or cellular component: Human airway ciliary transition zone (TZ) linker

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Supramolecule #1: Human airway ciliary transition zone (TZ) linker

SupramoleculeName: Human airway ciliary transition zone (TZ) linker / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1-#8
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statecell

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Sample preparation

BufferpH: 7.2
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 4.68 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 2.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionResolution.type: BY AUTHOR / Resolution: 7.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 5) / Number subtomograms used: 12780
ExtractionNumber tomograms: 209 / Number images used: 66385 / Software - Name: Warp (ver. 2.0)
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Final angle assignmentType: MAXIMUM LIKELIHOOD

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