登録情報 データベース : EMDB / ID : EMD-73236 ダウンロードとリンクタイトル Tra1, Core and minimal HAT modules of ctSAGA, composite. マップデータ 詳細 試料複合体 : Tra1 module of ctSAGA complexタンパク質・ペプチド : x 8種 詳細 キーワード ctSAGA complex / Histone Acetyl Transferase (HAT) Module / Tra1 module and Core module / TRANSCRIPTION機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
SAGA-type complex / RITS complex assembly / regulatory ncRNA-mediated heterochromatin formation / SLIK (SAGA-like) complex / histone H3 acetyltransferase activity / SAGA complex / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / NuA4 histone acetyltransferase complex / histone acetyltransferase complex ... SAGA-type complex / RITS complex assembly / regulatory ncRNA-mediated heterochromatin formation / SLIK (SAGA-like) complex / histone H3 acetyltransferase activity / SAGA complex / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / NuA4 histone acetyltransferase complex / histone acetyltransferase complex / histone acetyltransferase / RNA polymerase II preinitiation complex assembly / translation initiation factor activity / TBP-class protein binding / transcription coregulator activity / transcription initiation at RNA polymerase II promoter / promoter-specific chromatin binding / chromatin organization / transcription by RNA polymerase II / transcription coactivator activity / chromatin remodeling / protein heterodimerization activity / DNA repair / chromatin binding / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / structural molecule activity / positive regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / nucleus 類似検索 - 分子機能 Histone acetyltransferases subunit 3 / Histone acetyltransferases subunit 3 / ADA2 ZZ-type zing finger / Transcriptional activator Spt7 / Transcriptional adaptor 2 / ADA2-like, zinc finger, ZZ-type / : / Transcriptional adapter 2-alpha-like domain / Transcription factor Spt20 / Transcriptional coactivator Hfi1/Transcriptional adapter 1 ... Histone acetyltransferases subunit 3 / Histone acetyltransferases subunit 3 / ADA2 ZZ-type zing finger / Transcriptional activator Spt7 / Transcriptional adaptor 2 / ADA2-like, zinc finger, ZZ-type / : / Transcriptional adapter 2-alpha-like domain / Transcription factor Spt20 / Transcriptional coactivator Hfi1/Transcriptional adapter 1 / Spt20-like, SEP domain / Spt20, SEP domain / Transcriptional regulator of RNA polII, SAGA, subunit / SAGA-associated factor 73 / LisH / SCA7 domain / SCA7, zinc-binding domain / SCA7 domain profile. / Tra1, HEAT repeat ring region / Tra1, HEAT repeat central region / Tra1 HEAT repeat central region / Tra1 HEAT repeat ring region / Histone acetyltransferase GCN5 / Transcription initiation factor IID, subunit 13 / Transcription initiation factor IID, 18kD subunit / Bromodomain associated / Bromodomain associated domain / TFIID subunit TAF5, NTD2 domain superfamily / WD40 associated region in TFIID subunit, NTD2 domain / Transcription initiation factor TFIID subunit 12 / Transcription initiation factor TFIID subunit A / TATA box binding protein associated factor (TAF) / TFIID subunit TAF5, NTD2 domain / Transcription initiation factor TFIID subunit 6 / TAF6, C-terminal HEAT repeat domain superfamily / TAF6 C-terminal HEAT repeat domain / Transcription initiation factor TFIID subunit 12 domain / TAF6, C-terminal HEAT repeat domain / Transcription initiation factor TFIID 23-30kDa subunit / Transcription initiation factor TFIID, 23-30kDa subunit / Myb-like domain profile. / Lissencephaly type-1-like homology motif / SWIRM domain / SWIRM domain / SWIRM domain profile. / LIS1 homology (LisH) motif profile. / LIS1 homology motif / : / FATC domain / PIK-related kinase, FAT / FAT domain / Myb-type HTH DNA-binding domain profile. / SANT domain profile. / FATC / FATC domain / PIK-related kinase / FAT domain profile. / FATC domain profile. / SANT domain / Zinc finger ZZ-type signature. / Zinc-binding domain, present in Dystrophin, CREB-binding protein. / Myb domain / Zinc finger, ZZ-type / Zinc finger, ZZ-type superfamily / Zinc finger ZZ-type profile. / Myb-like DNA-binding domain / Acetyltransferase (GNAT) family / Gcn5-related N-acetyltransferase (GNAT) domain profile. / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / Phosphatidylinositol 3-/4-kinase, catalytic domain superfamily / Phosphoinositide 3-kinase, catalytic domain / GNAT domain / Phosphatidylinositol 3- and 4-kinase / Phosphatidylinositol 3- and 4-kinases catalytic domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain / SANT/Myb domain / Acyl-CoA N-acyltransferase / TATA box binding protein associated factor / TATA box binding protein associated factor (TAF), histone-like fold domain / Homeobox-like domain superfamily / Bromodomain, conserved site / Bromodomain signature. / Bromodomain / Histone-fold / bromo domain / Bromodomain / Bromodomain (BrD) profile. / Bromodomain-like superfamily / Armadillo-like helical / WD domain, G-beta repeat / Armadillo-type fold / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / Winged helix-like DNA-binding domain superfamily / WD40-repeat-containing domain superfamily 類似検索 - ドメイン・相同性 Spt20-like SEP domain-containing protein / Transcription initiation factor TFIID subunit 10 / SAGA complex subunit Spt7 / Transcription initiation factor TFIID subunit 12 domain-containing protein / Uncharacterized protein / SCA7 domain-containing protein / TBP-associated factor 6 / Transcriptional adapter 2 / Non-specific serine/threonine protein kinase / histone acetyltransferase ... Spt20-like SEP domain-containing protein / Transcription initiation factor TFIID subunit 10 / SAGA complex subunit Spt7 / Transcription initiation factor TFIID subunit 12 domain-containing protein / Uncharacterized protein / SCA7 domain-containing protein / TBP-associated factor 6 / Transcriptional adapter 2 / Non-specific serine/threonine protein kinase / histone acetyltransferase / Uncharacterized protein / Putative transcriptional coactivator HFI1 protein / Transcription initiation factor TFIID subunit-like protein 類似検索 - 構成要素生物種 Thermochaetoides thermophila (菌類)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.5 Å 詳細 データ登録者Mattoo RUH / Chen DH / Bushnell DA / Tamir S / Kornberg RD 資金援助 米国, 1件 詳細 詳細を隠すOrganization Grant number 国 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM049985 米国
引用ジャーナル : Mol Cell / 年 : 2025タイトル : Structure of the transcriptional co-activator SAGA complex, including the histone acetyltransferase module.著者 : Rayees U H Mattoo / Dong-Hua Chen / David A Bushnell / Sagi Tamir / Roger D Kornberg / 要旨 : The Spt-Ada-Gcn5 acetyltransferase (SAGA) complex, a 1.8 MDa multi-subunit assembly comprising 19 subunits, is required for RNA polymerase II transcription in eukaryotes. The complex consists of four ... The Spt-Ada-Gcn5 acetyltransferase (SAGA) complex, a 1.8 MDa multi-subunit assembly comprising 19 subunits, is required for RNA polymerase II transcription in eukaryotes. The complex consists of four modules: transcription-associated protein 1 (Tra1), core, deubiquitination (DUB), and histone acetyltransferase (HAT). Although the structures of the Tra1, core, and DUB modules have been determined, the overall architecture of the HAT module remained elusive due to its inherent flexibility. To address this, we conducted cryo-electron microscopy (cryo-EM) analyses on SAGA purified from the thermophilic fungus Chaetomium thermophilum, yielding structures of Tra1 and core modules at 2.6 Å and three of the four HAT subunits at 3.7 Å. The structure of the HAT module was informative about the aspects of histone acetylation and the interface of HAT-core modules, contradicting earlier AlphaFold predictions. Our structure-guided genetic and biochemical analyses confirmed the roles of Ada1 and Spt7 in anchoring the HAT module within the SAGA complex. 履歴 登録 2025年10月12日 - ヘッダ(付随情報) 公開 2025年12月3日 - マップ公開 2025年12月3日 - 更新 2025年12月3日 - 現状 2025年12月3日 処理サイト : RCSB / 状態 : 公開
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