National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01GM085234
United States
National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS)
RO1NS053494
United States
National Basic Research Program of China
2014CB910301
China
National Natural Science Foundation of China
31370821
China
Top Talents of Program of Yunnan Province
2011HA012
China
High-level Overseas Talents of Yunnan Province
China
China Youth 1000-talent Program of the State Council of China
China
Beijing Advanced Innovation Center for Structural Biology
China
Tsinghua-Peking Joint Center for Life Sciences
China
National Natural Science Foundation of China
31570730
China
Key Research Program of the Chinese Academy of Sciences
KJZD-EW-L03
China
National Natural Science Foundation of China
81302865
China
West Light Foundation of the Chinese Academy of Sciences
China
Yunnan Applied Basic Research Projects
2013FB074
China
Youth Innovation Promotion Association of the Chinese Academy of Sciences
China
Citation
Journal: Nature / Year: 2017 Title: Structure of a eukaryotic cyclic-nucleotide-gated channel. Authors: Minghui Li / Xiaoyuan Zhou / Shu Wang / Ioannis Michailidis / Ye Gong / Deyuan Su / Huan Li / Xueming Li / Jian Yang / Abstract: Cyclic-nucleotide-gated channels are essential for vision and olfaction. They belong to the voltage-gated ion channel superfamily but their activities are controlled by intracellular cyclic ...Cyclic-nucleotide-gated channels are essential for vision and olfaction. They belong to the voltage-gated ion channel superfamily but their activities are controlled by intracellular cyclic nucleotides instead of transmembrane voltage. Here we report a 3.5-Å-resolution single-particle electron cryo-microscopy structure of a cyclic-nucleotide-gated channel from Caenorhabditis elegans in the cyclic guanosine monophosphate (cGMP)-bound open state. The channel has an unusual voltage-sensor-like domain, accounting for its deficient voltage dependence. A carboxy-terminal linker connecting S6 and the cyclic-nucleotide-binding domain interacts directly with both the voltage-sensor-like domain and the pore domain, forming a gating ring that couples conformational changes triggered by cyclic nucleotide binding to the gate. The selectivity filter is lined by the carboxylate side chains of a functionally important glutamate and three rings of backbone carbonyls. This structure provides a new framework for understanding mechanisms of ion permeation, gating and channelopathy of cyclic-nucleotide-gated channels and cyclic nucleotide modulation of related channels.
History
Deposition
Oct 26, 2016
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Header (metadata) release
Jan 25, 2017
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Map release
Jan 25, 2017
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Update
May 29, 2024
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Current status
May 29, 2024
Processing site: PDBj / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 20 sec. / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.039 kPa
Vitrification
Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 281.15 K / Instrument: FEI VITROBOT MARK IV Details: Blotted for 4.0 seconds (double-sided, blot force 1), after waiting for 3.0 seconds the grid was immediately plunged into liquid ethane cooled by liquid-nitrogen..
Details
This sample was homogeneous
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Electron microscopy
Microscope
FEI TITAN KRIOS
Image recording
Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Dimensions - Width: 7676 pixel / Digitization - Dimensions - Height: 7420 pixel / Digitization - Frames/image: 1-32 / Average exposure time: 8.0 sec. / Average electron dose: 50.0 e/Å2
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
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