|Entry||Database: EMDB / ID: 6584|
|Title||The structure of elongation factor 4 (EF4/LepA) in GTP form bound to the ribosome|
|Keywords||LepA / EF4 / ribosome / 70S|
|Sample||The structure of elongation factor 4 (EF4/LepA) in GTP form bound to the ribosome|
|Source||Thermus thermophilus / bacteria / thermophilic /|
|Map data||Reconstruction of EF4 bound to the ribosome|
|Method||single particle reconstruction, at 3.8 Å resolution|
|Authors||Kumar V / Ahmed T / Ero R / Jian G-K / Yin Z / Gao Y / Bhushan S|
|Citation||J. Biol. Chem., 2016, 291, 12943-12950|
|Validation Report||PDB-ID: 5imq|
SummaryFull reportAbout validation report
|Date||Deposition: Feb 3, 2016 / Header (metadata) release: Mar 9, 2016 / Map release: May 18, 2016 / Last update: Jun 29, 2016|
Downloads & links
|File||emd_6584.map.gz (map file in CCP4 format, 132862 KB)|
|Projections & slices|
Images are generated by Spider package.
|Voxel size||X=Y=Z: 1.28 Å|
CCP4 map header:
-Entire The structure of elongation factor 4 (EF4/LepA) in GTP form bound...
|Entire||Name: The structure of elongation factor 4 (EF4/LepA) in GTP form bound to the ribosome|
Number of components: 1 / Oligomeric State: 1
|Mass||Theoretical: 2.5 MDa / Experimental: 2.5 MDa / Measured by: Sedimentation|
-Component #1: ribosome-prokaryote, Thermus thermophilus
|Ribosome-prokaryote||Name: Thermus thermophilus / a.k.a: 70S ribosome / Prokaryote: ALL / Recombinant expression: No|
|Mass||Theoretical: 2.5 MDa / Experimental: 2.5 MDa|
|Source||Species: Thermus thermophilus / bacteria / thermophilic /|
|Source (natural)||Location in cell: Translation|
|Sample solution||Buffer solution: 5mM HEPES, pH 7.5, 10mM MgOAc, 50mM KCl, 10mM NH4Cl, 6mM 2-mercaptoethanol|
|Support film||300 mesh copper grid with 2 nm carbon support, glow-discharged|
|Vitrification||Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 4 K / Humidity: 100 % / Method: Blot for 3 seconds before plunging|
-Electron microscopy imaging
Model: Tecnai F20 / Image courtesy: FEI Company
|Imaging||Microscope: FEI TECNAI F20 / Date: Nov 17, 2015|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Electron dose: 20 e/Å2 / Illumination mode: SPOT SCAN|
|Lens||Magnification: 78000 X (nominal), 109375 X (calibrated)|
Astigmatism: Objective lens astigmatism was corrected at 73684 times magnification
Cs: 2.7 mm / Imaging mode: BRIGHT FIELD / Defocus: 500 - 2500 nm
|Specimen Holder||Model: FEI TITAN KRIOS AUTOGRID HOLDER|
|Camera||Detector: FEI FALCON II (4k x 4k)|
|Image acquisition||Number of digital images: 2026|
Details: Every image is the average of seven frames recorded by the direct electron detector
|Processing||Method: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 110981|
Details: Particles were selected using EMAN and processed using Relion
|3D reconstruction||Algorithm: Every image is the average of two frames recorded by the direct electron detector|
Software: Relion / CTF correction: Ctffind3 / Resolution: 3.8 Å / Resolution method: FSC 0.143, gold-standard
-Atomic model buiding
-Oct 4, 2017. Three pioneers of this field were awarded Nobel Prize in Chemistry 2017
Three pioneers of this field were awarded Nobel Prize in Chemistry 2017
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External links: The 2017 Nobel Prize in Chemistry - Press Release
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