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基本情報
登録情報 | データベース: EMDB / ID: EMD-6532 | |||||||||
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タイトル | Negative stain EM reconstruction of Bundibugyo virus (BDBV) glycoprotein in complex with a human IgG1 Fab | |||||||||
![]() | Reconstruction of Bundibugyo virus (BDBV) glycoprotein (GP) ectodomain (missing transmembrane and cytoplasmic tail, GPdTM) in complex with Fab of BDBV432 human IgG1 | |||||||||
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![]() | Ebola virus / EBOV / GP / antibody / Bundibugyo virus / BDBV / Fab | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / 解像度: 22.0 Å | |||||||||
![]() | Flyak AI / Shen X / Murin CD / Turner HL / Fusco ML / Lampley R / Kose N / Ilinykh PA / Kuzmina N / Branchizio A ...Flyak AI / Shen X / Murin CD / Turner HL / Fusco ML / Lampley R / Kose N / Ilinykh PA / Kuzmina N / Branchizio A / King H / Brown L / Davidson E / Doranz BJ / Slaughter JC / Sapparapu G / Klages C / Ksiazek TG / Saphire EO / Ward AB / Bukreyev A / Crowe JE | |||||||||
![]() | ![]() タイトル: Cross-Reactive and Potent Neutralizing Antibody Responses in Human Survivors of Natural Ebolavirus Infection. 著者: Andrew I Flyak / Xiaoli Shen / Charles D Murin / Hannah L Turner / Joshua A David / Marnie L Fusco / Rebecca Lampley / Nurgun Kose / Philipp A Ilinykh / Natalia Kuzmina / Andre Branchizio / ...著者: Andrew I Flyak / Xiaoli Shen / Charles D Murin / Hannah L Turner / Joshua A David / Marnie L Fusco / Rebecca Lampley / Nurgun Kose / Philipp A Ilinykh / Natalia Kuzmina / Andre Branchizio / Hannah King / Leland Brown / Christopher Bryan / Edgar Davidson / Benjamin J Doranz / James C Slaughter / Gopal Sapparapu / Curtis Klages / Thomas G Ksiazek / Erica Ollmann Saphire / Andrew B Ward / Alexander Bukreyev / James E Crowe / ![]() 要旨: Recent studies have suggested that antibody-mediated protection against the Ebolaviruses may be achievable, but little is known about whether or not antibodies can confer cross-reactive protection ...Recent studies have suggested that antibody-mediated protection against the Ebolaviruses may be achievable, but little is known about whether or not antibodies can confer cross-reactive protection against viruses belonging to diverse Ebolavirus species, such as Ebola virus (EBOV), Sudan virus (SUDV), and Bundibugyo virus (BDBV). We isolated a large panel of human monoclonal antibodies (mAbs) against BDBV glycoprotein (GP) using peripheral blood B cells from survivors of the 2007 BDBV outbreak in Uganda. We determined that a large proportion of mAbs with potent neutralizing activity against BDBV bind to the glycan cap and recognize diverse epitopes within this major antigenic site. We identified several glycan cap-specific mAbs that neutralized multiple ebolaviruses, including SUDV, and a cross-reactive mAb that completely protected guinea pigs from the lethal challenge with heterologous EBOV. Our results provide a roadmap to develop a single antibody-based treatment effective against multiple Ebolavirus infections. | |||||||||
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構造の表示
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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マップデータ | ![]() | 1.6 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 11.8 KB 11.8 KB | 表示 表示 | ![]() |
画像 | ![]() | 120.9 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 79.2 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 78.3 KB | 表示 | |
XML形式データ | ![]() | 493 B | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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リンク
EMDBのページ | ![]() ![]() |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Reconstruction of Bundibugyo virus (BDBV) glycoprotein (GP) ectodomain (missing transmembrane and cytoplasmic tail, GPdTM) in complex with Fab of BDBV432 human IgG1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 4.1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
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試料の構成要素
-全体 : Bundibugyo virus (BDBV) glycoprotein (GP) ectodomain (missing tra...
全体 | 名称: Bundibugyo virus (BDBV) glycoprotein (GP) ectodomain (missing transmembrane and cytoplasmic tail, GPdTM) in complex with Fab of BDBV432 human IgG1 |
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要素 |
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-超分子 #1000: Bundibugyo virus (BDBV) glycoprotein (GP) ectodomain (missing tra...
超分子 | 名称: Bundibugyo virus (BDBV) glycoprotein (GP) ectodomain (missing transmembrane and cytoplasmic tail, GPdTM) in complex with Fab of BDBV432 human IgG1 タイプ: sample / ID: 1000 集合状態: Three Fabs (each consisting of one heavy chain and one light chain) bind to a single GP (consisting of a trimer of heterodimers of GP1 and GP2) Number unique components: 2 |
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分子量 | 理論値: 360 KDa |
-分子 #1: human IgG1 monoclonal antibody Fab
分子 | 名称: human IgG1 monoclonal antibody Fab / タイプ: protein_or_peptide / ID: 1 / Name.synonym: Fab 詳細: Fabs were generated by optimized papain proteolysis of IgG1. There are three Fabs bound to each trimeric GP. コピー数: 3 集合状態: heterodimer (one heavy chain, one light chain) 組換発現: No / データベース: NCBI |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 50 KDa |
-分子 #2: Bundibugyo virus glycoprotein, mucin-deleted
分子 | 名称: Bundibugyo virus glycoprotein, mucin-deleted / タイプ: protein_or_peptide / ID: 2 / Name.synonym: BDBV GPdTM 詳細: Recombinantly expressed ectodomain of Bundibugyo virus GP. Mucin-like domains were deleted from the expression construct, as were the transmembrane domains and cytoplasmic tail. コピー数: 1 集合状態: trimer of heterodimers (consisting of covalently linked GP1 and GP2) 組換発現: Yes |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 210 KDa |
組換発現 | 生物種: ![]() ![]() 組換細胞: S2 / 組換プラスミド: pMT puro |
-実験情報
-構造解析
![]() | 単粒子再構成法 |
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試料の集合状態 | particle |
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試料調製
濃度 | 0.03 mg/mL |
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緩衝液 | pH: 7.4 / 詳細: 20 mM Tris, 150 mM NaCl |
凍結 | 凍結剤: NONE / 装置: OTHER |
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電子顕微鏡法
顕微鏡 | FEI TECNAI SPIRIT |
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日付 | 2015年3月5日 |
撮影 | カテゴリ: CCD フィルム・検出器のモデル: TVIPS TEMCAM-F416 (4k x 4k) 実像数: 83 |
電子線 | 加速電圧: 120 kV / 電子線源: LAB6 |
電子光学系 | 倍率(補正後): 52000 / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最小 デフォーカス(公称値): 1.0 µm |
試料ステージ | 試料ホルダーモデル: SIDE ENTRY, EUCENTRIC |
実験機器 | ![]() モデル: Tecnai Spirit / 画像提供: FEI Company |
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画像解析
最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 22.0 Å / 解像度の算出法: OTHER / ソフトウェア - 名称: EMAN2 / 使用した粒子像数: 18119 |
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