Entire Complex of VIC91 Fab bound to Ebola virus GP
Entire
Name: Complex of VIC91 Fab bound to Ebola virus GP Details: Fab fragment generated by proteolytic cleavage of IgG antibody with papain. Number of components: 1
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Component #1: protein, Complex of VIC91 Fab bound to Ebola virus GP
Protein
Name: Complex of VIC91 Fab bound to Ebola virus GP Details: Fab fragment generated by proteolytic cleavage of IgG antibody with papain. Recombinant expression: No
Specimen conc.: 0.02 mg/ml / Buffer solution: Solution was made from 10X concentration. / pH: 7.4
Staining
Stained using 2% UF on carbon coated grids.
Vitrification
Cryogen name: NONE
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Electron microscopy imaging
Experimental equipment
Model: Tecnai Spirit / Image courtesy: FEI Company
Imaging
Microscope: FEI TECNAI SPIRIT
Electron gun
Electron source: LAB6 / Accelerating voltage: 120 kV / Electron dose: 3 e/Å2 / Illumination mode: FLOOD BEAM
Lens
Magnification: 52000. X (nominal) / Cs: 2.2 mm / Imaging mode: BRIGHT FIELD / Defocus: 1000.0 - 1.0 nm
Specimen Holder
Model: SIDE ENTRY, EUCENTRIC
Camera
Detector: TVIPS TEMCAM-F416 (4k x 4k)
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Image acquisition
Image acquisition
Number of digital images: 75
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Image processing
Processing
Method: single particle reconstruction / Applied symmetry: C3 (3 fold cyclic) / Number of projections: 12820
3D reconstruction
Resolution: 26 Å / Resolution method: FSC 0.5 CUT-OFF
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