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- EMDB-61549: Human VANGL2 in complex with a PK1 peptide (residues 745-790) -

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Basic information

Entry
Database: EMDB / ID: EMD-61549
TitleHuman VANGL2 in complex with a PK1 peptide (residues 745-790)
Map data
Sample
  • Complex: VANGL2-PK1 (745-790) complex
    • Protein or peptide: Vang-like protein 2
KeywordsVANGL / Planar cell polarity / PCP / Prickle1 / MEMBRANE PROTEIN
Function / homology
Function and homology information


cell migration involved in kidney development / establishment of planar polarity involved in neural tube closure / orthogonal dichotomous subdivision of terminal units involved in lung branching morphogenesis / planar dichotomous subdivision of terminal units involved in lung branching morphogenesis / lateral sprouting involved in lung morphogenesis / dopaminergic neuron axon guidance / serotonergic neuron axon guidance / regulation of establishment of planar polarity / apical cytoplasm / establishment of body hair planar orientation ...cell migration involved in kidney development / establishment of planar polarity involved in neural tube closure / orthogonal dichotomous subdivision of terminal units involved in lung branching morphogenesis / planar dichotomous subdivision of terminal units involved in lung branching morphogenesis / lateral sprouting involved in lung morphogenesis / dopaminergic neuron axon guidance / serotonergic neuron axon guidance / regulation of establishment of planar polarity / apical cytoplasm / establishment of body hair planar orientation / muscular septum morphogenesis / convergent extension involved in axis elongation / kidney morphogenesis / convergent extension involved in neural plate elongation / establishment of planar polarity / cell pole / heparan sulfate proteoglycan biosynthetic process / inner ear receptor cell stereocilium organization / somatic stem cell division / membranous septum morphogenesis / regulation of synapse pruning / cochlea morphogenesis / establishment or maintenance of epithelial cell apical/basal polarity / post-anal tail morphogenesis / glomerulus development / COPII-coated ER to Golgi transport vesicle / non-motile cilium assembly / PCP/CE pathway / apical protein localization / regulation of Wnt signaling pathway / RND1 GTPase cycle / Wnt signaling pathway, planar cell polarity pathway / anterior/posterior pattern specification / RND2 GTPase cycle / RND3 GTPase cycle / digestive tract morphogenesis / heart looping / lateral plasma membrane / somatic stem cell population maintenance / hair follicle development / Rho protein signal transduction / stress fiber / Asymmetric localization of PCP proteins / regulation of actin cytoskeleton organization / neural tube closure / wound healing / postsynaptic density membrane / cell-cell junction / basolateral plasma membrane / apical plasma membrane / glutamatergic synapse / plasma membrane
Similarity search - Function
Vang-like protein / Strabismus protein
Similarity search - Domain/homology
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.5 Å
AuthorsSong Y / Zhang Z / Jian S / Zheng P
Funding support China, 2 items
OrganizationGrant numberCountry
Ministry of Science and Technology (MoST, China)YFA1302300 China
National Natural Science Foundation of China (NSFC)32171201 China
CitationJournal: Nat Commun / Year: 2025
Title: Structural basis of human VANGL-PRICKLE interaction.
Authors: Yanyi Song / Shuyi Jian / Junlin Teng / Pengli Zheng / Zhe Zhang /
Abstract: Planar cell polarity (PCP) is an evolutionarily conserved process for development and morphogenesis in metazoans. The well-organized polarity pattern in cells is established by the asymmetric ...Planar cell polarity (PCP) is an evolutionarily conserved process for development and morphogenesis in metazoans. The well-organized polarity pattern in cells is established by the asymmetric distribution of two core protein complexes on opposite sides of the cell membrane. The Van Gogh-like (VANGL)-PRICKLE (PK) pair is one of these two key regulators; however, their structural information and detailed functions have been unclear. Here, we present five cryo-electron microscopy structures of human VANGL1, VANGL2, and their complexes with PK1 at resolutions of 2.2-3.0 Å. Through biochemical and cell imaging experiments, we decipher the molecular details of the VANGL-PK interaction. Furthermore, we reveal that PK1 can target VANGL-containing intracellular vesicles to the peripheral cell membrane. These findings provide a solid foundation to understand the explicit interaction between VANGL and PK while opening new avenues for subsequent studies of the PCP pathway.
History
DepositionSep 15, 2024-
Header (metadata) releaseDec 25, 2024-
Map releaseDec 25, 2024-
UpdateJan 22, 2025-
Current statusJan 22, 2025Processing site: PDBc / Status: Released

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Structure visualization

Downloads & links

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Map

FileDownload / File: emd_61549.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.07 Å
Density
Contour LevelBy AUTHOR: 0.08
Minimum - Maximum-0.4075225 - 0.6897652
Average (Standard dev.)0.000031487038 (±0.014643321)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 410.88 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : VANGL2-PK1 (745-790) complex

EntireName: VANGL2-PK1 (745-790) complex
Components
  • Complex: VANGL2-PK1 (745-790) complex
    • Protein or peptide: Vang-like protein 2

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Supramolecule #1: VANGL2-PK1 (745-790) complex

SupramoleculeName: VANGL2-PK1 (745-790) complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 350 KDa

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Macromolecule #1: Vang-like protein 2

MacromoleculeName: Vang-like protein 2 / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 60.374074 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: GPSRATMDTE SQYSGYSYKS GHSRSSRKHR DRRDRHRSKS RDGGRGDKSV TIQAPGEPLL DNESTRGDER DDNWGETTTV VTGTSEHSI SHDDLTRIAK DMEDSVPLDC SRHLGVAAGA TLALLSFLTP LAFLLLPPLL WREELEPCGT ACEGLFISVA F KLLILLLG ...String:
GPSRATMDTE SQYSGYSYKS GHSRSSRKHR DRRDRHRSKS RDGGRGDKSV TIQAPGEPLL DNESTRGDER DDNWGETTTV VTGTSEHSI SHDDLTRIAK DMEDSVPLDC SRHLGVAAGA TLALLSFLTP LAFLLLPPLL WREELEPCGT ACEGLFISVA F KLLILLLG SWALFFRRPK ASLPRVFVLR ALLMVLVFLL VVSYWLFYGV RILDARERSY QGVVQFAVSL VDALLFVHYL AV VLLELRQ LQPQFTLKVV RSTDGASRFY NVGHLSIQRV AVWILEKYYH DFPVYNPALL NLPKSVLAKK VSGFKVYSLG EEN STNNST GQSRAVIAAA ARRRDNSHNE YYYEEAEHER RVRKRRARLV VAVEEAFTHI KRLQEEEQKN PREVMDPREA AQAI FASMA RAMQKYLRTT KQQPYHTMES ILQHLEFCIT HDMTPKAFLE RYLAAGPTIQ YHKERWLAKQ WTLVSEEPVT NGLKD GIVF LLKRQDFSLV VSTKKVPFFK LSEEFVDPKS HKFVMRLQSE TSV

UniProtKB: Vang-like protein 2

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration3 mg/mL
BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 43582
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION

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